##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197549
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.636252044801505	31.0	31.0	34.0	30.0	34.0
2	31.839588192215935	31.0	31.0	34.0	30.0	34.0
3	31.913033203651793	31.0	31.0	34.0	30.0	34.0
4	35.620929080981234	37.0	35.0	37.0	33.0	37.0
5	35.506567163431306	37.0	35.0	37.0	33.0	37.0
6	35.55592965298288	37.0	35.0	37.0	33.0	37.0
7	35.42474838190337	37.0	35.0	37.0	33.0	37.0
8	35.453966393024416	37.0	35.0	37.0	33.0	37.0
9	37.05965684911432	39.0	37.0	39.0	33.0	39.0
10	36.88442644100576	39.0	37.0	39.0	33.0	39.0
11	36.97453465369684	39.0	37.0	39.0	33.0	39.0
12	36.83063072993255	39.0	37.0	39.0	32.0	39.0
13	36.8723551186632	39.0	37.0	39.0	33.0	39.0
14	37.8674050080623	40.0	37.0	41.0	32.0	41.0
15	37.88433542176562	40.0	37.0	41.0	33.0	41.0
16	37.77611688540511	40.0	37.0	41.0	32.0	41.0
17	37.79932345148299	40.0	37.0	41.0	32.0	41.0
18	37.77387480595784	40.0	37.0	41.0	32.0	41.0
19	37.81908464705828	40.0	37.0	41.0	32.0	41.0
20	37.71024818191155	40.0	37.0	41.0	32.0	41.0
21	37.642289376050584	39.0	37.0	41.0	32.0	41.0
22	37.51371843657337	39.0	36.0	41.0	32.0	41.0
23	37.34055725485972	39.0	36.0	41.0	31.0	41.0
24	37.19520871379793	39.0	36.0	41.0	31.0	41.0
25	37.3879089707394	39.0	36.0	41.0	31.0	41.0
26	37.26815437197142	39.0	36.0	41.0	31.0	41.0
27	37.15895717001977	39.0	36.0	41.0	31.0	41.0
28	36.98870275871801	39.0	36.0	41.0	31.0	41.0
29	36.881815274364556	39.0	35.0	41.0	30.0	41.0
30	36.69121931545181	39.0	35.0	40.0	30.0	41.0
31	36.54548749153479	39.0	35.0	40.0	30.0	41.0
32	36.38127709179332	38.0	35.0	40.0	30.0	41.0
33	36.23925451067138	38.0	35.0	40.0	30.0	41.0
34	36.072005404371765	38.0	35.0	40.0	30.0	41.0
35	35.93367119007239	38.0	35.0	40.0	29.0	41.0
36	35.74658239454085	38.0	35.0	40.0	27.0	41.0
37	35.68423588512871	38.0	35.0	40.0	27.0	41.0
38	35.50299820717148	38.0	35.0	40.0	26.0	41.0
39	35.40639088671946	38.0	34.0	40.0	26.0	41.0
40	35.24557993034105	38.0	34.0	40.0	25.0	41.0
41	35.10851831532572	38.0	34.0	40.0	24.0	41.0
42	34.85742629320387	38.0	34.0	40.0	23.0	41.0
43	34.50147927141186	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	5.0
16	14.0
17	31.0
18	81.0
19	193.0
20	381.0
21	805.0
22	1467.0
23	2611.0
24	4262.0
25	6532.0
26	9685.0
27	13580.0
28	18167.0
29	24260.0
30	30717.0
31	38695.0
32	47896.0
33	59444.0
34	73451.0
35	91993.0
36	118449.0
37	165389.0
38	223834.0
39	265603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.23338585728016	21.291988887302317	14.155579437668104	23.31904581774942
2	18.943692491914735	23.144689695369458	35.271124605339736	22.640493207376068
3	19.71743953692083	24.474489144076777	32.52843933734653	23.279631981655864
4	13.965942103412887	16.557234818784032	35.89907385835569	33.57774921944739
5	13.012661694845054	38.14031826672645	34.916483584387784	13.930536454040713
6	32.47065464544666	36.952558934957985	13.859975666966445	16.71681075262891
7	26.020062644618296	32.95372464926278	21.691471497199696	19.33474120891922
8	26.5114830374373	34.88500261784695	19.1523687130965	19.451145631619248
9	24.580873099973363	14.684075557659854	21.935803879423723	38.79924746294306
10	16.539699001878002	27.839445400563985	33.49958957838051	22.121266019177504
11	33.06111065183971	22.665293862714595	23.374826416288602	20.898769069157087
12	20.844324532858362	27.322806832956314	30.93961082177013	20.893257812415193
13	31.25258340159776	21.083646681680666	26.33503931780662	21.328730598914948
14	20.44935113302253	23.26627135925127	27.814978760785568	28.469398746940627
15	22.84841789354757	29.651980837527315	25.37750021084732	22.12210105807779
16	21.78558038126206	28.366521954425245	26.870048741220607	22.977848923092083
17	21.621745749025717	27.252246045882046	27.315959513973958	23.810048691118276
18	22.820694602058037	25.927957853916624	29.340511327720204	21.91083621630514
19	22.64992914694931	25.376080644716836	30.621711512430807	21.35227869590305
20	23.511104764815467	24.971170281967588	30.300889566940477	21.21683538627647
21	22.298127258258326	25.8537228956811	30.836316509804607	21.011833336255968
22	21.223599201368796	26.197758922599405	30.349906350387336	22.228735525644463
23	21.980060941138944	26.396999204207926	31.001153188721297	20.621786665931833
24	21.984737158980554	26.45929310616935	29.105113861729247	22.450855873120847
25	21.931628684922284	26.603170308688828	30.009962014080426	21.455238992308455
26	21.58316695183245	27.242726602418777	29.985495374302012	21.188611071446765
27	20.745288919284306	27.229867003354354	29.790012767744784	22.23483130961656
28	21.21182515287475	26.81869384885295	29.913765532767343	22.05571546550496
29	21.36530530274753	26.787713905652293	29.376083984872437	22.47089680672774
30	21.534567687835736	26.99605611127394	30.70730299971024	20.762073201180076
31	22.13078546264078	26.6766537319141	30.453618181802998	20.738942623642124
32	21.679363433145532	26.692769982689647	29.664088901581483	21.963777682583345
33	21.305516517486968	26.574528474408982	30.202020961146474	21.917934046957576
34	20.725247985677413	26.795312759644908	30.742291129632278	21.737148125045405
35	20.41962374817231	28.369611598356308	29.829343099948314	21.38142155352307
36	22.179802246087636	26.538538297806603	29.71352320447848	21.568136251627283
37	21.38142155352307	26.865289019488976	29.92311796845056	21.830171458537396
38	21.097424823535405	25.91518175874223	31.093007467752887	21.89438594996948
39	20.650344996321653	25.62817888871353	31.721791759669127	21.99968435529569
40	19.803615551430465	26.07717930539794	31.3748330965998	22.74437204657179
41	20.870795265997465	25.253580438044704	31.420175708885402	22.45544858707243
42	19.795682681877736	26.9877892261611	30.8159415606376	22.40058653132356
43	19.920688005250724	26.832137975147575	30.338549821343424	22.908624198258277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	484.0
1	424.5
2	365.0
3	670.5
4	976.0
5	976.0
6	1501.5
7	2027.0
8	2223.5
9	2420.0
10	3783.0
11	5146.0
12	5146.0
13	9431.0
14	13716.0
15	21092.5
16	28469.0
17	26298.5
18	24128.0
19	24128.0
20	26199.0
21	28270.0
22	22936.5
23	17603.0
24	19904.5
25	22206.0
26	22206.0
27	24516.0
28	26826.0
29	28952.5
30	31079.0
31	34102.5
32	37126.0
33	37126.0
34	41417.0
35	45708.0
36	49910.5
37	54113.0
38	57443.5
39	60774.0
40	60774.0
41	63028.0
42	65282.0
43	69599.0
44	73916.0
45	78230.5
46	82545.0
47	82545.0
48	102070.0
49	121595.0
50	113044.5
51	104494.0
52	94487.5
53	84481.0
54	84481.0
55	76390.5
56	68300.0
57	59730.5
58	51161.0
59	46445.0
60	41729.0
61	41729.0
62	36715.5
63	31702.0
64	27751.5
65	23801.0
66	20238.0
67	16675.0
68	16675.0
69	14196.0
70	11717.0
71	9610.5
72	7504.0
73	6159.0
74	4814.0
75	4814.0
76	3874.5
77	2935.0
78	2279.0
79	1623.0
80	1240.5
81	858.0
82	858.0
83	667.5
84	477.0
85	363.0
86	249.0
87	193.0
88	137.0
89	137.0
90	107.5
91	78.0
92	52.5
93	27.0
94	16.5
95	6.0
96	6.0
97	5.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1197549.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.6762125670239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.41310587553006	49.530413437952966
2	9.167234136513134	10.75797157691445
3	2.586437449318697	4.552870607026049
4	1.0722581075060638	2.5166417857096226
5	0.6151526047856762	1.8047412499781392
6	0.38122737666907536	1.3421387153882132
7	0.2824208062887962	1.159996828420618
8	0.19781886749641758	0.9285809535190188
9	0.15912561861198277	0.8403199760282288
>10	0.9915686326210557	10.94029102454386
>50	0.08212701776637155	3.3179144023677845
>100	0.04424456802545169	4.724237089773851
>500	0.004424453036998511	1.7589006177958697
>1k	0.002283588664257296	2.427271128229086
>5k	2.85448583032162E-4	1.2309708223102171
>10k+	2.85448583032162E-4	2.1667397840419245
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13398	1.1187851186047502	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12475	1.0417110281082445	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9590	0.8008023053753959	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5109	0.4266213741567151	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2965	0.24758903393514586	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2788	0.23280884540006294	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2638	0.22028326189575542	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2420	0.20207941386949513	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2405	0.20082685551906435	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2247	0.18763324089452707	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1603	0.13385673571603335	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1537	0.12834547897413803	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1490	0.12442079614278831	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1460	0.12191567944192681	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1418	0.11840851606072068	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1290	0.10772001813704492	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1271	0.1061334442264993	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1202	0.10037167581451781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	8.350389002871699E-5	0.0	0.0	0.0	0.0
7	8.350389002871699E-5	0.0	0.0	0.0	0.0
8	8.350389002871699E-5	0.0	0.0	8.350389002871699E-5	0.0
9	8.350389002871699E-5	0.0	0.0	8.350389002871699E-5	0.0
10	8.350389002871699E-5	0.0	0.0	1.6700778005743398E-4	0.0
11	8.350389002871699E-5	0.0	0.0	5.845272302010189E-4	0.0
12	8.350389002871699E-5	0.0	0.0	7.515350102584528E-4	0.0
13	8.350389002871699E-5	0.0	0.0	0.0010855505703733209	0.0
14	8.350389002871699E-5	0.0	0.0	0.0015030700205169057	0.0
15	8.350389002871699E-5	0.0	0.0	0.0016700778005743397	0.0
16	8.350389002871699E-5	0.0	0.0	0.0029226361510050944	0.0
17	8.350389002871699E-5	0.0	0.0	0.0035071633812061133	0.0
18	8.350389002871699E-5	0.0	0.0	0.00417519450143585	0.0
19	8.350389002871699E-5	0.0	0.0	0.004759721731636868	0.0
20	1.6700778005743398E-4	0.0	0.0	0.008767908453015283	0.0
21	1.6700778005743398E-4	0.0	0.0	0.014863692425111624	0.0
22	1.6700778005743398E-4	8.350389002871699E-5	0.0	0.02329758531801204	0.0
23	1.6700778005743398E-4	8.350389002871699E-5	0.0	0.0299778965203094	0.0
24	1.6700778005743398E-4	8.350389002871699E-5	0.0	0.03874580497332468	0.0
25	1.6700778005743398E-4	8.350389002871699E-5	0.0	0.045092100615507176	0.0
26	1.6700778005743398E-4	8.350389002871699E-5	0.0	0.05861973080015932	0.0
27	1.6700778005743398E-4	8.350389002871699E-5	0.0	0.12792795952399444	0.0
28	1.6700778005743398E-4	8.350389002871699E-5	0.0	0.2764813798850819	0.0
29	1.6700778005743398E-4	8.350389002871699E-5	0.0	0.4600229301682019	0.0
30	1.6700778005743398E-4	1.6700778005743398E-4	0.0	0.6986770478702751	0.0
31	1.6700778005743398E-4	1.6700778005743398E-4	0.0	1.029769971834138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4035	0.0	21.044611	1
ATTAGAG	150	2.5102054E-10	17.266666	3
GTATTGG	415	0.0	16.939758	1
CGGTTGG	110	7.8082667E-7	16.818182	33
TTGGACC	615	0.0	16.544716	4
ATTGGAC	470	0.0	16.138298	3
TTAACGG	140	3.4729965E-8	15.857142	35
GTATTAG	235	0.0	15.744681	1
TACACTG	230	0.0	15.282609	5
CTAGACC	85	5.3643854E-4	15.235293	3
TAAGAGT	85	5.3643854E-4	15.235293	6
GTCTAGA	110	1.4518902E-5	15.136364	1
AATCTAA	110	1.4518902E-5	15.136364	21
TGGACCC	600	0.0	15.108334	5
ATAGGCG	75	0.0041045398	14.8	32
ATAGGAC	75	0.0041045398	14.8	3
TGAACCG	75	0.0041045398	14.8	5
GTCTAAC	75	0.0041045398	14.8	31
CTAGTAC	150	8.1006874E-8	14.8	3
TGCGACG	75	0.0041045398	14.8	22
>>END_MODULE