FastQCFastQC Report
Wed 25 May 2016
SRR2088673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763174
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT192541.0920079356887067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT175120.9932088381521053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138380.7848346221076309No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68090.38617856207044793No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG50370.28567798753838247No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA43900.24898280033621187No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG42770.24257390365329798No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA39620.22470839520092745No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG34910.1979952063721448No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34790.19731461557395924No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA25720.1458732944111018No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA23500.13328236464466922No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC23300.1321480466476933No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA22100.12534213866583785No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.11178703860197575No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18040.10231548332722692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53350.023.3373951
TATTCCG656.904805E-519.9230775
GTATTGG6250.019.5361
ATTGGAC6200.019.0967753
TTGGACC8400.018.5000024
ATAGGAC1306.9667294E-1018.53
GCGTAGA500.007036338618.4999981
TATACCG1054.800859E-717.619055
GGACCCT8650.017.537576
ATCGCGT752.068055E-417.26666622
TGGACCC9300.016.3118295
GACCCTC8550.016.228077
ACGTTTA1151.2430392E-616.08695626
TAAGGTC2250.015.6222225
CGCACTA1101.452664E-515.13636429
ACTTAGC1853.0559022E-1015.08
TATTGGA7800.014.9423082
CGCGTAT750.004105561414.824
GTATCAA84300.014.7473312
TCACGTT1405.999573E-714.53571424
Produced by FastQC (version 0.11.5)