##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088673_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1763174
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.609750370638405	31.0	31.0	34.0	30.0	34.0
2	31.8212677818525	31.0	31.0	34.0	30.0	34.0
3	31.899108652917977	31.0	31.0	34.0	30.0	34.0
4	35.61408006243286	37.0	35.0	37.0	33.0	37.0
5	35.4975992159594	37.0	35.0	37.0	33.0	37.0
6	35.54377957025228	37.0	35.0	37.0	33.0	37.0
7	35.41103487233818	37.0	35.0	37.0	33.0	37.0
8	35.430707916518735	37.0	35.0	37.0	33.0	37.0
9	37.0388974655933	39.0	37.0	39.0	33.0	39.0
10	36.85165559383248	39.0	37.0	39.0	32.0	39.0
11	36.946825440937765	39.0	37.0	39.0	33.0	39.0
12	36.797706295578315	39.0	37.0	39.0	32.0	39.0
13	36.833221224904634	39.0	37.0	39.0	32.0	39.0
14	37.8216438082685	40.0	37.0	41.0	32.0	41.0
15	37.83250603740754	40.0	37.0	41.0	32.0	41.0
16	37.72708706004059	39.0	37.0	41.0	32.0	41.0
17	37.75240617205109	39.0	37.0	41.0	32.0	41.0
18	37.71386091219585	39.0	37.0	41.0	32.0	41.0
19	37.76113304756082	40.0	37.0	41.0	32.0	41.0
20	37.64742731006696	39.0	37.0	41.0	32.0	41.0
21	37.57938411070036	39.0	37.0	41.0	32.0	41.0
22	37.44168414461647	39.0	36.0	41.0	32.0	41.0
23	37.269384076670825	39.0	36.0	41.0	31.0	41.0
24	37.12756199898592	39.0	36.0	41.0	31.0	41.0
25	37.31004483959042	39.0	36.0	41.0	31.0	41.0
26	37.19779670072268	39.0	36.0	41.0	31.0	41.0
27	37.09819393888522	39.0	36.0	41.0	31.0	41.0
28	36.939116048671316	39.0	36.0	41.0	30.0	41.0
29	36.836888474988854	39.0	35.0	41.0	30.0	41.0
30	36.64463235052241	39.0	35.0	40.0	30.0	41.0
31	36.50587576722433	39.0	35.0	40.0	30.0	41.0
32	36.33674044649025	38.0	35.0	40.0	30.0	41.0
33	36.20164203873242	38.0	35.0	40.0	30.0	41.0
34	36.02449843293969	38.0	35.0	40.0	29.0	41.0
35	35.884821917746066	38.0	35.0	40.0	29.0	41.0
36	35.71356598951663	38.0	35.0	40.0	27.0	41.0
37	35.64246637030718	38.0	35.0	40.0	27.0	41.0
38	35.46872174839239	38.0	34.0	40.0	26.0	41.0
39	35.38123009980864	38.0	34.0	40.0	26.0	41.0
40	35.22163949786011	38.0	34.0	40.0	25.0	41.0
41	35.082388351915355	38.0	34.0	40.0	24.0	41.0
42	34.83136774929758	38.0	33.0	40.0	24.0	41.0
43	34.48468160260984	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	2.0
15	8.0
16	16.0
17	49.0
18	110.0
19	238.0
20	547.0
21	1179.0
22	2225.0
23	3890.0
24	6255.0
25	9631.0
26	14367.0
27	20040.0
28	27279.0
29	35897.0
30	46299.0
31	57670.0
32	71542.0
33	88973.0
34	109801.0
35	136914.0
36	177634.0
37	245170.0
38	331195.0
39	376240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39886364023063	21.120377228793075	13.732450682689286	23.74830844828701
2	18.8850334680525	23.224310249583986	34.87506054422309	23.01559573814042
3	19.509985968486376	24.429806700870135	32.82069721990002	23.239510110743467
4	13.59241912596261	16.413014257242903	36.583627027168056	33.41093958962644
5	12.80242335696874	38.22850155458282	35.242409427543734	13.726665660904711
6	32.32477339162215	37.262062621159345	13.954323282897773	16.458840704320732
7	26.23722899725155	32.835500069760556	21.627927816539945	19.29934311644795
8	26.93778379218387	34.24636479439919	19.188747111742803	19.62710430167414
9	24.69574755526114	14.258093642487923	21.69689435075608	39.34926445149486
10	16.19857143991461	27.61962234016609	34.04207412314383	22.139732096775475
11	33.372883220827894	22.451839693643397	23.441759009604272	20.73351807592444
12	21.16711113026848	26.98860123844839	30.916007155277924	20.928280476005202
13	31.461387248223943	20.96990994649422	26.00928779575924	21.5594150095226
14	20.671981324588497	22.82089005395951	27.668965173034536	28.838163448417458
15	22.994327275697124	29.543198799437832	25.134671904190963	22.327802020674078
16	22.11063684015304	28.17974856707279	26.54808884432279	23.16152574845137
17	21.849176541850095	26.941016598475247	26.971926763892846	24.23788009578181
18	22.903014676940565	25.811519452986488	29.282816103232012	22.002649766840936
19	22.79729623962241	25.04687569122503	30.348337713691333	21.80749035546123
20	23.705034216702376	24.774015497052474	29.987511158853298	21.53343912739185
21	22.693335995199565	25.53888612241333	30.4345458814615	21.3332320009256
22	21.562817963513528	25.890864996874953	30.04933148968848	22.49698554992304
23	22.435618946286638	26.003729637574057	30.74863853482413	20.812012881315173
24	22.30284702474061	26.059254503526024	28.693311040203636	22.94458743152973
25	22.336366121551247	26.178528040908045	29.782256317300504	21.702849520240203
26	21.925516143046575	26.85582931690236	29.660657428024688	21.55799711202638
27	21.03394219742351	26.90250650247792	29.438841543716048	22.624709756382526
28	21.49680065608953	26.60956887975889	29.5117781909216	22.38185227322998
29	21.738637253044793	26.61790611703666	28.93639538695557	22.707061242962975
30	21.749469989915912	26.844259273333204	30.362573404553377	21.043697332197503
31	22.481842404663407	26.564139443980007	29.98076196677129	20.9732561845853
32	21.948202502986092	26.51672495170641	29.201769082348083	22.333303462959414
33	21.59531617412689	26.51899358770036	29.853491487510592	22.032198750662157
34	21.01885576806373	27.00890553059426	30.444301016235492	21.52793768510652
35	20.730001690133815	28.874915351519476	29.243285121037406	21.151797837309307
36	22.58636980808474	26.990699726742797	29.039561608780527	21.38336885639194
37	21.738467105345247	27.58610324335545	29.121232504562794	21.554197146736513
38	21.52141535662391	26.506686237433176	30.087047563087932	21.884850842854988
39	21.19314372829908	26.28566437572242	30.724874572787485	21.796317323191015
40	20.304462293568303	26.787543373484407	30.421614656295976	22.48637967665131
41	21.670634889126088	25.786734604752564	30.364104733849295	22.17852577227205
42	20.375470600178996	27.507778585664262	29.874533086354493	22.242217727802245
43	20.799365235648892	27.235428834590348	29.19161693627515	22.77358899348561
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	593.0
1	503.5
2	414.0
3	784.5
4	1155.0
5	1155.0
6	1874.0
7	2593.0
8	2749.0
9	2905.0
10	4623.5
11	6342.0
12	6342.0
13	11794.5
14	17247.0
15	27638.5
16	38030.0
17	35630.0
18	33230.0
19	33230.0
20	36098.5
21	38967.0
22	31948.0
23	24929.0
24	28303.5
25	31678.0
26	31678.0
27	35725.5
28	39773.0
29	43603.5
30	47434.0
31	52447.5
32	57461.0
33	57461.0
34	63889.0
35	70317.0
36	76736.5
37	83156.0
38	86619.0
39	90082.0
40	90082.0
41	92808.5
42	95535.0
43	100236.5
44	104938.0
45	110365.5
46	115793.0
47	115793.0
48	144911.0
49	174029.0
50	164299.0
51	154569.0
52	141526.5
53	128484.0
54	128484.0
55	116386.5
56	104289.0
57	90737.5
58	77186.0
59	69735.0
60	62284.0
61	62284.0
62	55202.0
63	48120.0
64	41992.5
65	35865.0
66	30822.0
67	25779.0
68	25779.0
69	22006.0
70	18233.0
71	15490.5
72	12748.0
73	10483.0
74	8218.0
75	8218.0
76	6602.5
77	4987.0
78	3848.5
79	2710.0
80	2112.0
81	1514.0
82	1514.0
83	1157.0
84	800.0
85	586.0
86	372.0
87	291.0
88	210.0
89	210.0
90	164.0
91	118.0
92	85.0
93	52.0
94	38.0
95	24.0
96	24.0
97	16.5
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1763174.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.381044775822495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.28572190964539	44.20402482803482
2	10.907405700083281	11.863122355285807
3	3.251364625916219	5.3043781591342585
4	1.3728680858427391	2.9863200339004687
5	0.752769147959887	2.046818637053218
6	0.46181542999959924	1.5068403346184354
7	0.29513639163408406	1.1234877729899466
8	0.22645144900164588	0.985173311016671
9	0.179912750971546	0.880545902969254
>10	1.1093302720570088	11.44873863137004
>50	0.09459975708969481	3.5507524882076105
>100	0.054483746466878785	5.49594902020157
>500	0.004279103418096531	1.6456012463729544
>1k	0.0033397880336363166	3.4137967775001887
>5k	2.087367521022698E-4	0.6723388412959239
>10k+	3.131051281534047E-4	2.872111660048872
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19254	1.0920079356887067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17512	0.9932088381521053	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13838	0.7848346221076309	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6809	0.38617856207044793	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5037	0.28567798753838247	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4390	0.24898280033621187	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4277	0.24257390365329798	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3962	0.22470839520092745	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3491	0.1979952063721448	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3479	0.19731461557395924	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2572	0.1458732944111018	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2350	0.13328236464466922	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2330	0.1321480466476933	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2210	0.12534213866583785	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1971	0.11178703860197575	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1804	0.10231548332722692	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	5.6715899848795414E-5	0.0	0.0	0.0	0.0
13	5.6715899848795414E-5	0.0	0.0	5.6715899848795414E-5	0.0
14	1.1343179969759083E-4	0.0	0.0	2.8357949924397705E-4	0.0
15	1.1343179969759083E-4	0.0	0.0	2.8357949924397705E-4	0.0
16	1.1343179969759083E-4	0.0	0.0	6.238748983367496E-4	0.0
17	1.1343179969759083E-4	0.0	0.0	0.0010776020971271128	0.0
18	1.1343179969759083E-4	0.0	0.0	0.0013044656965222944	0.0
19	1.1343179969759083E-4	0.0	0.0	0.0018149087951614532	0.0
20	1.1343179969759083E-4	0.0	0.0	0.003346238091078929	0.0
21	1.1343179969759083E-4	0.0	0.0	0.005841737684425927	0.0
22	1.1343179969759083E-4	0.0	0.0	0.009414839374900038	0.0
23	1.1343179969759083E-4	0.0	0.0	0.011343179969759082	0.0
24	1.1343179969759083E-4	0.0	0.0	0.013781963663257285	0.0
25	1.1343179969759083E-4	0.0	0.0	0.01678790635524344	0.0
26	1.1343179969759083E-4	0.0	0.0	0.02115503064360069	0.0
27	1.1343179969759083E-4	0.0	0.0	0.051101025763764664	0.0
28	1.1343179969759083E-4	0.0	0.0	0.1223361959738517	0.0
29	1.1343179969759083E-4	0.0	0.0	0.2286217922904943	0.0
30	1.1343179969759083E-4	0.0	0.0	0.36973095111429727	0.0
31	1.1343179969759083E-4	0.0	0.0	0.5247355054010552	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5335	0.0	23.337395	1
TATTCCG	65	6.904805E-5	19.923077	5
GTATTGG	625	0.0	19.536	1
ATTGGAC	620	0.0	19.096775	3
TTGGACC	840	0.0	18.500002	4
ATAGGAC	130	6.9667294E-10	18.5	3
GCGTAGA	50	0.0070363386	18.499998	1
TATACCG	105	4.800859E-7	17.61905	5
GGACCCT	865	0.0	17.53757	6
ATCGCGT	75	2.068055E-4	17.266666	22
TGGACCC	930	0.0	16.311829	5
GACCCTC	855	0.0	16.22807	7
ACGTTTA	115	1.2430392E-6	16.086956	26
TAAGGTC	225	0.0	15.622222	5
CGCACTA	110	1.452664E-5	15.136364	29
ACTTAGC	185	3.0559022E-10	15.0	8
TATTGGA	780	0.0	14.942308	2
CGCGTAT	75	0.0041055614	14.8	24
GTATCAA	8430	0.0	14.747331	2
TCACGTT	140	5.999573E-7	14.535714	24
>>END_MODULE