FastQCFastQC Report
Wed 25 May 2016
SRR2088672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647648
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75831.1708520677899106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72221.1151119126439053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56170.8672921092939375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33720.520653194327783No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG20750.3203900884431049No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA18430.28456816048223726No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG18340.2831785167251346No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16160.24951825683087106No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15610.24102598942635506No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG15060.23253372202183903No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA10040.15502248134789268No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC9690.14961831118138247No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA8980.13865556598646178No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG8910.13757473195315975No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.13556746874845596No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA8750.13510425416275507No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA8740.13494984930085477No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.1346410395770542No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.13232496664854984No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG6640.10252482830179356No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC6580.10159839913039181No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC6550.10113518454469095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.022.1288451
GTATTGG2350.021.255321
TACTCCG1059.804353E-1021.1428575
GTTCCGA450.003824031720.55555533
TAGGGGT555.139761E-420.1818184
TTGTCGG851.2435903E-619.58823622
CTAAGGT851.2435903E-619.5882364
ATTGGAC3150.018.7936523
TGGACCC3950.018.7341775
TCGGGTA609.230882E-418.525
AGTTCCG500.00703164618.532
GTCGGGT701.2181545E-418.524
GGACCCT4000.018.03756
TCTACAC852.7201431E-517.4117643
TACACAG852.7201431E-517.4117645
TATTGGA3050.016.9836062
TTGGACC4250.016.5411784
TCCGGTC1151.2400706E-616.0869568
AGGACTA700.002590855115.8571425
TTCCGAC700.002590855115.85714234
Produced by FastQC (version 0.11.5)