FastQCFastQC Report
Wed 25 May 2016
SRR2088671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2601051
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT253660.9752211702115798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT229560.8825663164620763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT182600.7020239126414669No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99750.38349882412916936No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58560.22513976081207174No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG50920.1957670187935569No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48540.18661687141082586No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA46170.17750517002550123No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG40700.15647520944418236No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39290.15105432380987532No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA27920.10734122475876098No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC27120.10426554496624632No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.10384263899477558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76300.022.1854521
CAATGCG1757.2759576E-1216.91428619
ACGTTTA1554.0199666E-1016.70967926
TTAACGG3000.016.6535
GTATTGG8300.016.2710841
ACCGTTA1201.9376912E-615.4166678
CGTTCGC1651.6478225E-814.57575923
GTATCAA117000.014.4995742
TAACGGC3500.014.27142936
AACGGCC3900.014.2307737
TAACCCG1051.6569001E-414.0952385
TTCGCTA1051.6569001E-414.09523826
TAACACT3550.014.0704224
GACCGTT1458.921652E-714.0344837
GGACCGT2009.858923E-1013.8750016
GTATTAG6650.013.6315791
GGACCCT11250.013.4844456
ACCGGTC1102.4582306E-413.4545458
GTAACAC2800.013.2142863
TAAACGT1551.88993E-613.1290324
Produced by FastQC (version 0.11.5)