##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088671_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2601051
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.570545906250974	31.0	31.0	34.0	30.0	34.0
2	31.779045086005617	31.0	31.0	34.0	30.0	34.0
3	31.86126723389891	31.0	31.0	34.0	30.0	34.0
4	35.57595372024616	37.0	35.0	37.0	33.0	37.0
5	35.44986353593221	37.0	35.0	37.0	33.0	37.0
6	35.4949718402292	37.0	35.0	37.0	33.0	37.0
7	35.3594189425736	37.0	35.0	37.0	33.0	37.0
8	35.381724156888886	37.0	35.0	37.0	33.0	37.0
9	36.98252936985857	39.0	37.0	39.0	33.0	39.0
10	36.79271263808361	39.0	37.0	39.0	32.0	39.0
11	36.88303343533057	39.0	37.0	39.0	33.0	39.0
12	36.74019617454637	39.0	35.0	39.0	32.0	39.0
13	36.77476297081449	39.0	37.0	39.0	32.0	39.0
14	37.77653071777524	40.0	37.0	41.0	32.0	41.0
15	37.787179874596845	40.0	37.0	41.0	32.0	41.0
16	37.67748575479681	39.0	37.0	41.0	32.0	41.0
17	37.689715426571794	39.0	37.0	41.0	32.0	41.0
18	37.64110392299113	39.0	37.0	41.0	32.0	41.0
19	37.68763011567247	40.0	37.0	41.0	32.0	41.0
20	37.55784065748807	39.0	36.0	41.0	32.0	41.0
21	37.49444013208507	39.0	36.0	41.0	32.0	41.0
22	37.36276951124757	39.0	36.0	41.0	31.0	41.0
23	37.18589754679935	39.0	36.0	41.0	31.0	41.0
24	37.027756087827576	39.0	36.0	41.0	31.0	41.0
25	37.22113599464217	39.0	36.0	41.0	31.0	41.0
26	37.10632471258734	39.0	36.0	41.0	31.0	41.0
27	36.99588512489759	39.0	36.0	41.0	31.0	41.0
28	36.83085875671027	39.0	35.0	41.0	30.0	41.0
29	36.72060870778774	39.0	35.0	41.0	30.0	41.0
30	36.53136866597387	39.0	35.0	40.0	30.0	41.0
31	36.39447169624894	39.0	35.0	40.0	30.0	41.0
32	36.22266768317884	38.0	35.0	40.0	30.0	41.0
33	36.09394740818231	38.0	35.0	40.0	29.0	41.0
34	35.91515891076338	38.0	35.0	40.0	29.0	41.0
35	35.78898145403531	38.0	35.0	40.0	28.0	41.0
36	35.603056995037775	38.0	34.0	40.0	27.0	41.0
37	35.545048905231	38.0	34.0	40.0	26.0	41.0
38	35.36364531106849	38.0	34.0	40.0	25.0	41.0
39	35.28415859589066	38.0	34.0	40.0	25.0	41.0
40	35.13981156078831	38.0	34.0	40.0	24.0	41.0
41	35.01580860967355	38.0	34.0	40.0	24.0	41.0
42	34.77753377384757	38.0	33.0	40.0	23.0	41.0
43	34.43108035943932	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	7.0
15	13.0
16	33.0
17	94.0
18	182.0
19	428.0
20	918.0
21	1940.0
22	3696.0
23	6383.0
24	10273.0
25	15697.0
26	22930.0
27	31947.0
28	42820.0
29	55761.0
30	71032.0
31	87605.0
32	107847.0
33	132596.0
34	163061.0
35	200763.0
36	255981.0
37	351645.0
38	484604.0
39	552790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34797818266539	21.37520563802863	13.595927184818752	23.68088899448723
2	18.95814422708359	23.26847878030842	34.70301043693492	23.070366555673072
3	19.696730283258574	24.631889186332756	32.27237758890541	23.399002941503262
4	13.86539518064044	16.50705810843386	36.467335703913534	33.160211007012165
5	12.872335067632276	38.48863401755675	35.04333440597666	13.595696508834312
6	32.44361606135366	37.50729993375755	14.068197816959374	15.98088618792942
7	26.250542569138396	32.81458149032833	21.906606214180343	19.028269726352924
8	26.751763037326064	34.58121351715134	19.394736973631044	19.272286471891555
9	24.765835041296768	14.523936670215232	21.704918511786197	39.0053097767018
10	16.15416229824021	27.755741813597655	34.072572971464226	22.017522916697903
11	33.28542962056492	22.634158269099682	23.437448938909693	20.642963171425706
12	20.734579983245236	27.142720384952085	31.196351013494162	20.92634861830852
13	30.786478235144177	21.20865757726396	26.14454695428886	21.860317233303
14	20.459037519833327	22.889593475868026	27.78115461788331	28.870214386415338
15	22.890439287810967	29.620180457822627	25.28846993003982	22.20091032432659
16	22.015485278835364	28.474528181108326	26.494328638692586	23.015657901363717
17	21.588888491613584	27.23525990070937	27.21853589183757	23.95731571583948
18	22.608168774852935	26.217094551394805	29.268476473548578	21.906260200203686
19	22.611590468622104	25.493502434208327	30.26638078222995	21.628526314939613
20	23.421301619998992	25.295620885557412	29.932784862734334	21.35029263170926
21	22.350273024250583	26.103640413048417	30.310901247226603	21.235185315474396
22	21.500347359586566	26.364573397445877	29.89422352733568	22.240855715631874
23	22.171845150287325	26.468377590443247	30.504553736162805	20.855223523106623
24	22.19541254669747	26.588021534372068	28.655224368918564	22.5613415500119
25	22.233089624155774	26.63742464103933	29.578812564613305	21.550673170191587
26	21.88638361954456	27.268361904476308	29.472278705800083	21.372975770179053
27	21.078594768038	27.2444100480921	29.326799051614138	22.35019613225577
28	21.465438393941525	26.877250772860663	29.56797079334469	22.08934003985312
29	21.763587103828414	26.773523471858105	29.109771396254825	22.353118028058656
30	21.801840871247816	27.093394170279627	30.12040133007773	20.98436362839483
31	22.405904382497692	26.85302979449461	29.901451374848094	20.83961444815961
32	21.794420793748372	26.71420129785998	29.317956472210653	22.17342143618099
33	21.476587733189394	26.714047513870355	29.79853143979107	22.010833313149185
34	21.011237380581925	26.94637667619743	30.31736017479088	21.725025768429763
35	20.822429087318934	28.388447592915327	29.34348461448853	21.44563870527721
36	22.41097925415534	26.765642042389786	29.196082660432264	21.62729604302261
37	21.5657055551775	26.98117030385025	29.43344824841958	22.019675892552666
38	21.373129554168678	26.08676262018699	30.361227057831623	22.178880767812704
39	20.88659545699027	25.896608717014775	31.10211987385099	22.114675952143962
40	20.191222701900116	26.370609419038686	30.819541792913714	22.618626086147483
41	21.16356042230621	25.443868651556624	30.87336618928272	22.519204736854448
42	20.06365888250557	26.944262146340076	30.445385346154303	22.546693625000046
43	20.403444607583626	26.596941005770358	29.976613299777664	23.023001086868348
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	978.0
1	887.0
2	796.0
3	1357.0
4	1918.0
5	1918.0
6	3023.5
7	4129.0
8	4467.5
9	4806.0
10	7311.5
11	9817.0
12	9817.0
13	18110.0
14	26403.0
15	40207.5
16	54012.0
17	50429.0
18	46846.0
19	46846.0
20	51057.5
21	55269.0
22	46363.0
23	37457.0
24	42563.5
25	47670.0
26	47670.0
27	53879.0
28	60088.0
29	65642.0
30	71196.0
31	79226.0
32	87256.0
33	87256.0
34	97016.5
35	106777.0
36	115762.0
37	124747.0
38	131487.0
39	138227.0
40	138227.0
41	143153.0
42	148079.0
43	155415.5
44	162752.0
45	168869.0
46	174986.0
47	174986.0
48	210922.5
49	246859.0
50	232962.5
51	219066.0
52	201268.5
53	183471.0
54	183471.0
55	167484.5
56	151498.0
57	132654.0
58	113810.0
59	103100.5
60	92391.0
61	92391.0
62	81370.0
63	70349.0
64	61424.5
65	52500.0
66	44659.5
67	36819.0
68	36819.0
69	31580.5
70	26342.0
71	22067.0
72	17792.0
73	14432.5
74	11073.0
75	11073.0
76	8893.5
77	6714.0
78	5220.5
79	3727.0
80	2915.5
81	2104.0
82	2104.0
83	1619.0
84	1134.0
85	868.0
86	602.0
87	448.0
88	294.0
89	294.0
90	230.5
91	167.0
92	121.5
93	76.0
94	57.0
95	38.0
96	38.0
97	24.0
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2601051.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.40760485437816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.14472711247079	42.80337916784808
2	11.617495171573404	12.409251830420772
3	3.5630831810432375	5.708872157893139
4	1.4919766824879086	3.1873160444104096
5	0.7631688180612789	2.03795093360998
6	0.46205746590175556	1.4806429535337762
7	0.3139568545207777	1.1737378539298429
8	0.23572065657848779	1.007142054604676
9	0.17320913588641115	0.8325616577930764
>10	1.0577764036989759	10.757113917318385
>50	0.10275852312187146	3.794956134996001
>100	0.0656964197783735	6.641667565576687
>500	0.004908647341563407	1.7911249286507434
>1k	0.0030318115933160327	3.000719294356601
>5k	2.165579709511452E-4	0.8066404115916329
>10k+	2.165579709511452E-4	2.5669230934662384
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25366	0.9752211702115798	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22956	0.8825663164620763	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18260	0.7020239126414669	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9975	0.38349882412916936	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5856	0.22513976081207174	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5092	0.1957670187935569	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4854	0.18661687141082586	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4617	0.17750517002550123	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4070	0.15647520944418236	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3929	0.15105432380987532	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2792	0.10734122475876098	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2712	0.10426554496624632	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2701	0.10384263899477558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.8445997406433014E-5	0.0	0.0	0.0	0.0
7	3.8445997406433014E-5	0.0	0.0	7.689199481286603E-5	0.0
8	3.8445997406433014E-5	0.0	0.0	7.689199481286603E-5	0.0
9	3.8445997406433014E-5	0.0	0.0	3.075679792514641E-4	0.0
10	3.8445997406433014E-5	0.0	0.0	4.2290597147076316E-4	0.0
11	7.689199481286603E-5	0.0	0.0	4.997979662836292E-4	0.0
12	7.689199481286603E-5	0.0	0.0	5.382439636900622E-4	0.0
13	7.689199481286603E-5	0.0	0.0	6.920279533157943E-4	0.0
14	7.689199481286603E-5	0.0	0.0	7.689199481286603E-4	0.0
15	7.689199481286603E-5	0.0	0.0	9.611499351608254E-4	0.0
16	7.689199481286603E-5	0.0	0.0	0.0015762858936637536	0.0
17	7.689199481286603E-5	0.0	0.0	0.002345205841792414	0.0
18	7.689199481286603E-5	0.0	0.0	0.002768111813263177	0.0
19	7.689199481286603E-5	0.0	0.0	0.0035370317613918375	0.0
20	7.689199481286603E-5	0.0	0.0	0.005882237603184251	0.0
21	7.689199481286603E-5	0.0	0.0	0.010226635310111183	0.0
22	7.689199481286603E-5	0.0	0.0	0.01580130493404397	0.0
23	7.689199481286603E-5	0.0	0.0	0.019569012679874404	3.8445997406433014E-5
24	7.689199481286603E-5	0.0	0.0	0.02537435828824579	3.8445997406433014E-5
25	7.689199481286603E-5	0.0	0.0	0.02998787797701775	3.8445997406433014E-5
26	7.689199481286603E-5	0.0	0.0	0.03986849931047104	3.8445997406433014E-5
27	7.689199481286603E-5	0.0	0.0	0.09019430991549185	3.8445997406433014E-5
28	7.689199481286603E-5	0.0	0.0	0.18857761727855393	3.8445997406433014E-5
29	7.689199481286603E-5	0.0	0.0	0.31817907453563965	3.8445997406433014E-5
30	7.689199481286603E-5	0.0	0.0	0.4995288443017842	3.8445997406433014E-5
31	7.689199481286603E-5	0.0	0.0	0.7304355047248209	3.8445997406433014E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7630	0.0	22.185452	1
CAATGCG	175	7.2759576E-12	16.914286	19
ACGTTTA	155	4.0199666E-10	16.709679	26
TTAACGG	300	0.0	16.65	35
GTATTGG	830	0.0	16.271084	1
ACCGTTA	120	1.9376912E-6	15.416667	8
CGTTCGC	165	1.6478225E-8	14.575759	23
GTATCAA	11700	0.0	14.499574	2
TAACGGC	350	0.0	14.271429	36
AACGGCC	390	0.0	14.23077	37
TAACCCG	105	1.6569001E-4	14.095238	5
TTCGCTA	105	1.6569001E-4	14.095238	26
TAACACT	355	0.0	14.070422	4
GACCGTT	145	8.921652E-7	14.034483	7
GGACCGT	200	9.858923E-10	13.875001	6
GTATTAG	665	0.0	13.631579	1
GGACCCT	1125	0.0	13.484445	6
ACCGGTC	110	2.4582306E-4	13.454545	8
GTAACAC	280	0.0	13.214286	3
TAAACGT	155	1.88993E-6	13.129032	4
>>END_MODULE