FastQCFastQC Report
Wed 25 May 2016
SRR2088670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466288
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT276771.1222128153727382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT258381.0476473145066594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT193140.7831202195364045No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111680.45282627170873796No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG69980.28374626158826544No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA61540.24952479191400193No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59930.24299676274628104No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA50880.20630194040598665No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG49530.20082812712870515No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA47700.19340806913061248No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.13656150457691882No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32450.13157425247984014No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32220.13064167688445144No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG30740.12464075566195026No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC30460.12350544624147708No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA30330.12297833829625737No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28720.11645030912853647No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28600.1159637479483337No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA27440.1112603232063733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT659.401083E-825.6153859
GGTATCA92550.022.128041
GTATTGG7800.018.4999981
ATTGGAC8700.017.0114963
AGAACCG1201.04260835E-716.9583325
TTGGACC11650.016.5150224
GCGTCGG1802.0190782E-1015.4166669
GGCGTCG2055.456968E-1215.3414648
GGACCCT12200.015.1639356
GTATCAA135300.015.1500362
TACGGCT2700.015.0740734
CGTACAC1353.9772203E-715.0740733
TGGACCC13200.014.5757575
GACCCTC12200.014.5573777
TATGCCG1152.2117641E-514.47826113
ACCGTCG1803.3360266E-914.38888823
GTAATAC1551.2118107E-714.3225813
TAGGTCG1458.921106E-714.03448321
TCGTATG1203.3033743E-513.87499910
TATTGGA10900.013.7477062
Produced by FastQC (version 0.11.5)