FastQCFastQC Report
Wed 25 May 2016
SRR2088669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3742850
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT418971.1193876324191459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT394511.0540363626648142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT305460.8161160612901933No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164230.4387832801207636No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG100650.268912726932685No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG93090.24871421510346395No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA85420.22822180958360608No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA80500.21507674632966858No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG75730.20233244719932675No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68700.1835499686068103No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA51480.13754224721802905No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC48960.13080940994162205No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46640.12461092483000921No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45000.1202292370786967No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44460.11878648623375235No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43910.11731701778056827No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40070.10705745621651949No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA38580.10307653258880266No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG38490.10283607411464527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123650.023.9086131
GTATTGG10700.017.1168211
TTAACGG4050.016.44444535
GTATCAA189700.015.5548232
CTAATAC8700.015.0977023
ATTGGAC12700.015.0039363
TTGGACC18200.014.9423074
TAATACT9750.014.84
GGACCCT17500.014.6942846
TGGACCC17550.014.54700855
GCCGATA1551.2123928E-714.322582
GTATTAG8150.014.0736191
AATACTG9950.013.9447235
CGATACC2151.9826984E-1013.7674424
AACGGCC5400.013.70370437
ACGTTTA1907.1413524E-913.63157926
GACCCTC18250.013.5835617
TAGAACG1102.4586832E-413.4545454
CGATTCC3750.013.3231
CGAACTA5350.013.14018624
Produced by FastQC (version 0.11.5)