##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088669_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3742850
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.531891740251414	31.0	31.0	34.0	30.0	34.0
2	31.747480395954955	31.0	31.0	34.0	30.0	34.0
3	31.82347622800807	31.0	31.0	34.0	30.0	34.0
4	35.54388420588589	37.0	35.0	37.0	33.0	37.0
5	35.42485832988231	37.0	35.0	37.0	33.0	37.0
6	35.47462067675702	37.0	35.0	37.0	33.0	37.0
7	35.33688365817492	37.0	35.0	37.0	32.0	37.0
8	35.36857902400577	37.0	35.0	37.0	33.0	37.0
9	36.956074916173506	39.0	37.0	39.0	33.0	39.0
10	36.775606823677144	39.0	37.0	39.0	32.0	39.0
11	36.86129607117571	39.0	37.0	39.0	32.0	39.0
12	36.719116448695516	39.0	35.0	39.0	32.0	39.0
13	36.752968459863474	39.0	35.0	39.0	32.0	39.0
14	37.736505604018326	40.0	37.0	41.0	32.0	41.0
15	37.74473489453224	40.0	37.0	41.0	32.0	41.0
16	37.62693295216212	39.0	36.0	41.0	32.0	41.0
17	37.656745260964236	39.0	37.0	41.0	32.0	41.0
18	37.620351870900514	39.0	36.0	41.0	32.0	41.0
19	37.67107578449577	39.0	37.0	41.0	32.0	41.0
20	37.5464354702967	39.0	36.0	41.0	32.0	41.0
21	37.484339741106375	39.0	36.0	41.0	32.0	41.0
22	37.3604886650547	39.0	36.0	41.0	31.0	41.0
23	37.1834620676757	39.0	36.0	41.0	31.0	41.0
24	37.02428069519217	39.0	36.0	41.0	31.0	41.0
25	37.21522930387272	39.0	36.0	41.0	31.0	41.0
26	37.0899704770429	39.0	36.0	41.0	31.0	41.0
27	36.97965774743845	39.0	36.0	41.0	31.0	41.0
28	36.80668421122941	39.0	35.0	41.0	30.0	41.0
29	36.69582670959296	39.0	35.0	40.0	30.0	41.0
30	36.50089156658696	39.0	35.0	40.0	30.0	41.0
31	36.350929104826534	38.0	35.0	40.0	30.0	41.0
32	36.17957679308548	38.0	35.0	40.0	30.0	41.0
33	36.04337924309016	38.0	35.0	40.0	29.0	41.0
34	35.86558184271344	38.0	35.0	40.0	29.0	41.0
35	35.73815114150981	38.0	35.0	40.0	27.0	41.0
36	35.545998370225895	38.0	34.0	40.0	27.0	41.0
37	35.48123809396583	38.0	34.0	40.0	26.0	41.0
38	35.28904043710007	38.0	34.0	40.0	25.0	41.0
39	35.200097519270074	38.0	34.0	40.0	25.0	41.0
40	35.03506312034946	38.0	34.0	40.0	24.0	41.0
41	34.91186288523451	38.0	34.0	40.0	24.0	41.0
42	34.653585102261644	38.0	33.0	40.0	23.0	41.0
43	34.30663531800633	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	4.0
14	4.0
15	21.0
16	62.0
17	116.0
18	284.0
19	632.0
20	1399.0
21	3056.0
22	5335.0
23	9255.0
24	15084.0
25	23210.0
26	33396.0
27	46291.0
28	63063.0
29	82200.0
30	103380.0
31	128136.0
32	156546.0
33	193147.0
34	234916.0
35	288503.0
36	373393.0
37	511773.0
38	696061.0
39	773579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.89896736444153	21.63001456109649	14.140588054557357	23.330430019904618
2	19.032769146506006	23.475586785470963	34.77261979507594	22.719024272947085
3	19.871221128284596	24.48329481544812	32.4886650547043	23.15681900156298
4	14.021587827457685	16.640608092763536	36.05319475800526	33.284609321773516
5	12.755386937761331	38.571596510680365	34.89429178299959	13.77872476855872
6	32.398974043843594	37.29417422552333	13.736323924282297	16.570527806350775
7	26.010820631337083	33.13173116742589	21.56460985612568	19.292838345111345
8	26.732329641850463	34.72249756201825	19.156952589604177	19.388220206527112
9	24.510333034986708	14.570768264824933	21.934114378080874	38.98478432210749
10	16.27778297286827	27.8858890952082	33.85150353340369	21.984824398519844
11	32.97978812936666	22.6257798201905	23.422979814846975	20.97145223559587
12	20.798348851810786	27.369598033584037	31.066593638537476	20.7654594760677
13	31.2088381848057	21.195212204603443	26.299050189027078	21.29689942156378
14	20.35518388393871	23.186983181265614	28.00368702993708	28.454145904858596
15	22.730940326221997	29.66613676743658	25.5681900156298	22.034732890711624
16	21.659831411892007	28.543997221368745	26.88926887265052	22.90690249408873
17	21.477323430006546	27.209372536970488	27.331178112935326	23.982125920087633
18	22.62821112254031	26.06476348237306	29.582911417769882	21.72411397731675
19	22.468145931576206	25.26120469695553	30.851276433733656	21.419372937734614
20	23.381540804467186	24.89284903215464	30.596871368075128	21.128738795303047
21	22.1585155696862	25.84599970610631	31.08839520686108	20.907089517346407
22	21.145116689153987	26.02840081755881	30.610737806751544	22.21574468653566
23	21.98629386697303	26.23327143754091	31.24982299584541	20.530611699640648
24	22.004141229277156	26.30973723232296	29.20747558678547	22.47864595161441
25	21.884473061971494	26.547096463924554	30.196775184685464	21.37165528941849
26	21.581709125399094	27.128738795303043	30.167572838879465	21.121979240418398
27	20.656932551398	27.109288376504537	29.924656344764017	22.30912272733345
28	21.081635651976434	26.714321973896897	30.13187811427121	22.07216425985546
29	21.47515930373913	26.564462909280362	29.579919045647035	22.380458741333477
30	21.489506659363855	26.88176122473516	30.8840055038273	20.744726612073688
31	22.184271344029284	26.66411958801448	30.50718035721442	20.644428710741813
32	21.686148256008124	26.50090171927809	29.82361035040143	21.989339674312355
33	21.279212364909093	26.457485605888557	30.403516037244344	21.859785991958
34	20.720814352699147	26.741386911043723	31.072311206700775	21.465487529556356
35	20.405706881120004	28.33247391693496	29.981404544665164	21.28041465727988
36	22.20845077948622	26.562378935837668	29.829675247471847	21.399495037204268
37	21.349613262620732	26.95507434174493	29.95292357428163	21.742388821352712
38	21.13929225055773	25.863873786018676	31.05176536596444	21.945068597459155
39	20.72068076465795	25.624831345097988	31.73894224988979	21.915545640354274
40	19.773434682127256	26.13740865917683	31.410529409407268	22.678627249288645
41	21.02491416968353	25.223613022162255	31.480102061263477	22.27137074689074
42	19.801434735562474	26.99533777736217	30.8524520084962	22.35077547857916
43	20.218817211483227	26.733425063788292	30.267656999345416	22.780100725383065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1677.0
1	1411.5
2	1146.0
3	1973.0
4	2800.0
5	2800.0
6	4537.0
7	6274.0
8	6877.5
9	7481.0
10	11458.5
11	15436.0
12	15436.0
13	29070.5
14	42705.0
15	65364.0
16	88023.0
17	81908.5
18	75794.0
19	75794.0
20	82492.5
21	89191.0
22	72950.5
23	56710.0
24	63617.5
25	70525.0
26	70525.0
27	78017.5
28	85510.0
29	92665.5
30	99821.0
31	110443.5
32	121066.0
33	121066.0
34	134884.0
35	148702.0
36	161357.0
37	174012.0
38	183558.0
39	193104.0
40	193104.0
41	198408.0
42	203712.0
43	214088.0
44	224464.0
45	236720.0
46	248976.0
47	248976.0
48	312558.5
49	376141.0
50	350203.5
51	324266.0
52	295322.0
53	266378.0
54	266378.0
55	241649.5
56	216921.0
57	186572.0
58	156223.0
59	141032.5
60	125842.0
61	125842.0
62	111312.0
63	96782.0
64	84741.0
65	72700.0
66	62352.5
67	52005.0
68	52005.0
69	44442.5
70	36880.0
71	30834.0
72	24788.0
73	20392.0
74	15996.0
75	15996.0
76	12727.0
77	9458.0
78	7380.0
79	5302.0
80	4171.0
81	3040.0
82	3040.0
83	2276.0
84	1512.0
85	1136.0
86	760.0
87	561.5
88	363.0
89	363.0
90	297.0
91	231.0
92	156.5
93	82.0
94	56.5
95	31.0
96	31.0
97	21.5
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3742850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.72089974465716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.60355034624698	37.80914796248953
2	12.510366992552905	12.19032672026076
3	4.217903255154992	6.165001248812084
4	1.8329518946865413	3.5721226199120943
5	0.988441028144938	2.407886811787768
6	0.5855525705710496	1.7117188851611034
7	0.39069998342432244	1.3324678305858937
8	0.27439498647866906	1.0695016501331032
9	0.1997395007988529	0.8758339374121902
>10	1.1957576077390537	11.137336920643188
>50	0.11739543623692532	3.9525602241244613
>100	0.07292600205149635	7.0003368743190535
>500	0.005654259466268151	1.8841268880849849
>1k	0.004062283494957906	3.9737944010439628
>5k	3.2937433742901947E-4	1.2167149349649808
>10k+	2.7447861452418293E-4	3.701122090264749
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41897	1.1193876324191459	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39451	1.0540363626648142	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30546	0.8161160612901933	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16423	0.4387832801207636	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10065	0.268912726932685	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9309	0.24871421510346395	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8542	0.22822180958360608	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8050	0.21507674632966858	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7573	0.20233244719932675	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6870	0.1835499686068103	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5148	0.13754224721802905	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4896	0.13080940994162205	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4664	0.12461092483000921	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4500	0.1202292370786967	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4446	0.11878648623375235	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4391	0.11731701778056827	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4007	0.10705745621651949	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3858	0.10307653258880266	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3849	0.10283607411464527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.671760823971038E-5	0.0	0.0	0.0	0.0
8	2.671760823971038E-5	0.0	0.0	5.343521647942076E-5	0.0
9	2.671760823971038E-5	0.0	0.0	2.4045847415739342E-4	0.0
10	2.671760823971038E-5	0.0	0.0	3.206112988765246E-4	0.0
11	2.671760823971038E-5	0.0	0.0	6.145049895133388E-4	0.0
12	2.671760823971038E-5	0.0	0.0	8.282458554310218E-4	0.0
13	5.343521647942076E-5	0.0	0.0	9.351162883898634E-4	0.0
14	5.343521647942076E-5	0.0	0.0	0.0010687043295884153	0.0
15	5.343521647942076E-5	0.0	0.0	0.0015496212779032021	0.0
16	5.343521647942076E-5	0.0	0.0	0.0022175614838959618	0.0
17	5.343521647942076E-5	0.0	0.0	0.0029923721228475625	0.0
18	5.343521647942076E-5	0.0	0.0	0.0035534418958814806	0.0
19	5.343521647942076E-5	0.0	0.0	0.00424809971011395	0.0
20	5.343521647942076E-5	0.0	0.0	0.006599249235208464	0.0
21	5.343521647942076E-5	0.0	0.0	0.01050002003820618	0.0
22	5.343521647942076E-5	0.0	0.0	0.01611071776854536	0.0
23	5.343521647942076E-5	0.0	0.0	0.02025194704570047	0.0
24	5.343521647942076E-5	0.0	0.0	0.027118372363306036	0.0
25	5.343521647942076E-5	0.0	0.0	0.032729070093645216	0.0
26	8.015282471913115E-5	0.0	0.0	0.04288176122473516	0.0
27	8.015282471913115E-5	0.0	0.0	0.09837423353861362	0.0
28	8.015282471913115E-5	0.0	0.0	0.21277903202105347	0.0
29	8.015282471913115E-5	0.0	0.0	0.35454266134095674	0.0
30	8.015282471913115E-5	0.0	0.0	0.5679361983515235	0.0
31	8.015282471913115E-5	0.0	0.0	0.8426733638804654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12365	0.0	23.908613	1
GTATTGG	1070	0.0	17.116821	1
TTAACGG	405	0.0	16.444445	35
GTATCAA	18970	0.0	15.554823	2
CTAATAC	870	0.0	15.097702	3
ATTGGAC	1270	0.0	15.003936	3
TTGGACC	1820	0.0	14.942307	4
TAATACT	975	0.0	14.8	4
GGACCCT	1750	0.0	14.694284	6
TGGACCC	1755	0.0	14.5470085	5
GCCGATA	155	1.2123928E-7	14.32258	2
GTATTAG	815	0.0	14.073619	1
AATACTG	995	0.0	13.944723	5
CGATACC	215	1.9826984E-10	13.767442	4
AACGGCC	540	0.0	13.703704	37
ACGTTTA	190	7.1413524E-9	13.631579	26
GACCCTC	1825	0.0	13.583561	7
TAGAACG	110	2.4586832E-4	13.454545	4
CGATTCC	375	0.0	13.32	31
CGAACTA	535	0.0	13.140186	24
>>END_MODULE