FastQCFastQC Report
Wed 25 May 2016
SRR2088668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459290
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT351961.4311447612928934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347231.4119115679728702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT266231.0825482151352626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139990.5692293304165024No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG107170.4357761793037828No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG95960.39019391775675094No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA92830.37746666720882854No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83530.33965087484599216No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG80700.32814348856783865No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA70050.2848383069910421No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA48390.19676410671372632No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47510.19318583818907084No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC45730.18594797685510858No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44590.1813124926299867No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.16020884076298444No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG38900.15817573364670293No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA38290.15569534296483944No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.1480508602076209No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33490.13617751464853675No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33260.13524228537504726No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG32750.1331685161164401No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32590.13251792183923003No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC30870.12552403335922155No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30610.12446681765875517No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC27110.11023506784478447No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT26500.10775467716292102No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT26180.10645348860850083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98450.025.0111731
GTATTGG7550.021.3178831
GTACGAT609.240896E-418.520
ATTGGAC8850.018.186443
CGAACTA1900.017.52631624
CGAATTA1705.456968E-1217.41176415
GGACCCT13950.016.974916
TTGGACC14900.016.5134224
CGTAACG904.449273E-516.44444521
TGGACCC14700.016.2346945
GTATCAA156350.015.7726262
TATACTG2500.015.545
TCTATAC2400.015.4166673
CTGGTCG1101.4531277E-515.1363649
TTTTTAC16800.014.976191
CCGAATT2109.094947E-1214.9761914
GACCCTC15150.014.8976897
CGGGTAC750.00410617514.86
ATAAGAC1508.112511E-814.83
TATTGGA10950.014.6986292
Produced by FastQC (version 0.11.5)