##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088668_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2459290
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.563082027739714	31.0	31.0	34.0	30.0	34.0
2	31.769156138560316	31.0	31.0	34.0	30.0	34.0
3	31.82578223796299	31.0	31.0	34.0	30.0	34.0
4	35.5387050734155	37.0	35.0	37.0	33.0	37.0
5	35.4401038511115	37.0	35.0	37.0	33.0	37.0
6	35.50562398090506	37.0	35.0	37.0	33.0	37.0
7	35.35426850839063	37.0	35.0	37.0	33.0	37.0
8	35.40284960293418	37.0	35.0	37.0	33.0	37.0
9	36.97884552045509	39.0	37.0	39.0	33.0	39.0
10	36.81412074216542	39.0	37.0	39.0	32.0	39.0
11	36.90042857898011	39.0	37.0	39.0	33.0	39.0
12	36.75643580057659	39.0	37.0	39.0	32.0	39.0
13	36.793255777073874	39.0	37.0	39.0	32.0	39.0
14	37.74261351853584	40.0	37.0	41.0	32.0	41.0
15	37.751571795111595	40.0	37.0	41.0	32.0	41.0
16	37.628689987760694	39.0	36.0	41.0	32.0	41.0
17	37.708782616120914	39.0	37.0	41.0	32.0	41.0
18	37.6810067133197	39.0	37.0	41.0	32.0	41.0
19	37.74210198878538	40.0	37.0	41.0	32.0	41.0
20	37.62301599242058	39.0	37.0	41.0	32.0	41.0
21	37.56368830028179	39.0	37.0	41.0	32.0	41.0
22	37.44457424703878	39.0	36.0	41.0	32.0	41.0
23	37.25473449654169	39.0	36.0	41.0	31.0	41.0
24	37.09325537045245	39.0	36.0	41.0	31.0	41.0
25	37.26824896616503	39.0	36.0	41.0	31.0	41.0
26	37.13981921611522	39.0	36.0	41.0	31.0	41.0
27	37.025600478186796	39.0	36.0	41.0	31.0	41.0
28	36.83656543148632	39.0	35.0	41.0	30.0	41.0
29	36.72009929695156	39.0	35.0	41.0	30.0	41.0
30	36.492423016399044	39.0	35.0	40.0	30.0	41.0
31	36.289555522122235	38.0	35.0	40.0	30.0	41.0
32	36.111417929565036	38.0	35.0	40.0	30.0	41.0
33	35.94693631088648	38.0	35.0	40.0	29.0	41.0
34	35.751956458978	38.0	35.0	40.0	28.0	41.0
35	35.59646564658905	38.0	35.0	40.0	27.0	41.0
36	35.38405312102273	38.0	34.0	40.0	26.0	41.0
37	35.27296333494626	38.0	34.0	40.0	25.0	41.0
38	35.06765407902281	38.0	34.0	40.0	25.0	41.0
39	34.94796059025166	38.0	34.0	40.0	24.0	41.0
40	34.74813299773512	38.0	33.0	40.0	23.0	41.0
41	34.57536687417913	38.0	33.0	40.0	22.0	41.0
42	34.2616795904509	38.0	33.0	40.0	21.0	41.0
43	33.893283833952076	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	4.0
14	7.0
15	9.0
16	31.0
17	65.0
18	178.0
19	367.0
20	915.0
21	1765.0
22	3421.0
23	5924.0
24	9886.0
25	15173.0
26	22351.0
27	31055.0
28	42618.0
29	55428.0
30	69574.0
31	85478.0
32	104346.0
33	128463.0
34	156367.0
35	190803.0
36	246759.0
37	344237.0
38	445358.0
39	498704.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.273009689788516	21.44801955035803	14.583965290795312	22.695005469058145
2	18.967710192779215	23.637635252450913	35.15722017330205	22.23743438146782
3	19.852559071927264	24.350279958849914	33.39061273782271	22.40654823140012
4	13.621533044089961	16.37631186236678	35.83595265300147	34.16620244054178
5	12.234181410081773	38.852595667855354	34.95931752660321	13.953905395459664
6	31.984637842629375	36.95867506475446	13.050311268699502	18.00637582391666
7	25.3748032968865	33.85786141528653	21.192376661556793	19.574958626270185
8	27.129008778956525	34.506503909664985	18.782738107339924	19.581749204038566
9	24.028073143061615	14.59998617487161	22.367797209763793	39.004143472302985
10	16.453041324935246	27.861252637956486	33.404397204071095	22.281308833037176
11	32.19750415770405	22.38609517381033	24.02738188664208	21.389018781843543
12	21.04684685417336	27.727514851847484	31.112556876171578	20.113081417807578
13	32.17912486937286	20.96149701743186	26.81818736301941	20.041190750175865
14	20.02931740461678	23.574690256130832	28.413729165734825	27.98226317351756
15	22.015825705793134	29.687226801231247	26.586209841051684	21.710737651923928
16	21.000898633345397	28.672381053068165	27.575031818126373	22.75168849546007
17	21.172248901105604	26.98575605154333	27.21500920997524	24.626985837375827
18	23.036567464593443	25.348535552944142	30.298338138243153	21.316558844219266
19	22.37771877249125	24.419934208653718	31.97463495561727	21.227712063237764
20	23.633853673214627	23.798413363206453	31.85988638997434	20.707846573604577
21	22.069987679370875	25.02734529071399	32.79194401636244	20.110723013552693
22	20.276705878525917	25.229842759495625	32.20018785909755	22.293263502880915
23	21.760386127703523	25.455273676548924	33.300830727567714	19.48350946817984
24	21.601234502641006	25.48487571616198	30.389421337052564	22.52446844414445
25	21.327090339081607	26.166861167247458	31.652102842690372	20.853945650980567
26	21.156309341313957	26.895933379146015	31.41817353789102	20.529583741649013
27	19.673279686413558	26.99108279218799	31.251865375779186	22.083772145619264
28	20.447039592727982	26.20569351316843	31.337377861089987	22.009889033013593
29	20.79112264108747	26.004415908656565	30.586226105908615	22.61823534434735
30	21.045952287042194	26.373506174546314	32.44655977944854	20.133981758962953
31	22.01895669075221	26.12623968706415	31.989761272562404	19.86504234962123
32	21.589605129935876	26.01027125715145	30.77977790337862	21.620345709534053
33	20.920346929398324	25.956271932143014	31.603836879749846	21.519544258708816
34	19.866750159598908	26.64293352959594	32.43513371745504	21.055182593350114
35	19.43064868315652	29.00853498367415	30.89208674048201	20.66872959268732
36	22.120734032993262	26.198333665407496	30.7413521788809	20.939580122718347
37	21.177616303892588	27.177071431185425	30.609972797026785	21.035339467895206
38	20.841299724717295	25.496871048148044	32.203603479052894	21.458225748081762
39	20.30447812173432	25.19475133066861	33.02424683546877	21.476523712128298
40	18.915134042752175	25.8486799035494	32.43334458319271	22.802841470505715
41	20.879400152076414	24.896779151706387	32.32595586531072	21.89786483090648
42	19.357904110535966	27.347079848248885	31.483802235604585	21.811213805610564
43	19.831618068629563	27.391198272672195	30.45887227614474	22.318311382553503
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	549.0
1	623.0
2	697.0
3	1279.5
4	1862.0
5	1862.0
6	3143.0
7	4424.0
8	5014.0
9	5604.0
10	8730.5
11	11857.0
12	11857.0
13	22988.5
14	34120.0
15	53019.0
16	71918.0
17	66192.0
18	60466.0
19	60466.0
20	65027.0
21	69588.0
22	53995.0
23	38402.0
24	41541.5
25	44681.0
26	44681.0
27	48685.0
28	52689.0
29	55713.0
30	58737.0
31	64237.5
32	69738.0
33	69738.0
34	77818.0
35	85898.0
36	94726.0
37	103554.0
38	109267.5
39	114981.0
40	114981.0
41	120065.0
42	125149.0
43	132845.5
44	140542.0
45	153671.0
46	166800.0
47	166800.0
48	230669.5
49	294539.0
50	267525.5
51	240512.0
52	213831.0
53	187150.0
54	187150.0
55	163279.0
56	139408.0
57	115598.0
58	91788.0
59	81209.0
60	70630.0
61	70630.0
62	62329.0
63	54028.0
64	47106.0
65	40184.0
66	33892.0
67	27600.0
68	27600.0
69	23319.5
70	19039.0
71	16103.0
72	13167.0
73	10687.5
74	8208.0
75	8208.0
76	6505.5
77	4803.0
78	3801.5
79	2800.0
80	2191.5
81	1583.0
82	1583.0
83	1196.0
84	809.0
85	616.0
86	423.0
87	309.0
88	195.0
89	195.0
90	142.5
91	90.0
92	69.0
93	48.0
94	36.0
95	24.0
96	24.0
97	13.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2459290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.48272442574555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19841909833022	38.88237852524794
2	11.435383693471412	11.088371126264777
3	3.4786884161038722	5.05968875522992
4	1.4907894696257316	2.8911014013067087
5	0.7774513247780727	1.8846479166823051
6	0.4732797433909435	1.376753482506641
7	0.3228006402785878	1.0955178140956694
8	0.23430023428660163	0.9087610953443941
9	0.18003026068116368	0.7855521765209995
>10	1.2003322368125413	11.270454395617834
>50	0.12780935056806242	4.269286837965896
>100	0.06881828573828326	6.374223701742957
>500	0.005445922434898294	1.8100082195995706
>1k	0.00561348927904895	5.659164818289703
>5k	4.189171103767873E-4	1.7185308859399537
>10k+	4.189171103767873E-4	4.925558847644761
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35196	1.4311447612928934	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34723	1.4119115679728702	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26623	1.0825482151352626	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13999	0.5692293304165024	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10717	0.4357761793037828	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9596	0.39019391775675094	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9283	0.37746666720882854	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8353	0.33965087484599216	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8070	0.32814348856783865	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7005	0.2848383069910421	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4839	0.19676410671372632	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4751	0.19318583818907084	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4573	0.18594797685510858	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4459	0.1813124926299867	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3940	0.16020884076298444	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3890	0.15817573364670293	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3829	0.15569534296483944	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3641	0.1480508602076209	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3349	0.13617751464853675	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3326	0.13524228537504726	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3275	0.1331685161164401	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3259	0.13251792183923003	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3087	0.12552403335922155	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3061	0.12446681765875517	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2711	0.11023506784478447	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2650	0.10775467716292102	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2618	0.10645348860850083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.066214232563057E-5	0.0	0.0	0.0	0.0
3	4.066214232563057E-5	0.0	0.0	4.066214232563057E-5	0.0
4	4.066214232563057E-5	0.0	0.0	8.132428465126114E-5	0.0
5	8.132428465126114E-5	0.0	0.0	8.132428465126114E-5	0.0
6	8.132428465126114E-5	0.0	0.0	8.132428465126114E-5	0.0
7	1.219864269768917E-4	0.0	0.0	1.219864269768917E-4	0.0
8	2.439728539537834E-4	0.0	0.0	1.219864269768917E-4	4.066214232563057E-5
9	2.439728539537834E-4	0.0	0.0	3.2529713860504454E-4	4.066214232563057E-5
10	2.84634996279414E-4	0.0	0.0	5.286078502331974E-4	4.066214232563057E-5
11	2.84634996279414E-4	0.0	0.0	0.0010978778427920253	4.066214232563057E-5
12	2.84634996279414E-4	0.0	0.0	0.0017484721200021144	4.066214232563057E-5
13	2.84634996279414E-4	0.0	0.0	0.0019517828316302673	4.066214232563057E-5
14	2.84634996279414E-4	0.0	0.0	0.002358404254886573	4.066214232563057E-5
15	2.84634996279414E-4	0.0	0.0	0.003049660674422293	4.066214232563057E-5
16	3.659592809306751E-4	0.0	0.0	0.004554159940470624	4.066214232563057E-5
17	3.659592809306751E-4	0.0	0.0	0.006099321348844586	4.066214232563057E-5
18	3.659592809306751E-4	0.0	0.0	0.007400509903264764	4.066214232563057E-5
19	4.879457079075668E-4	0.0	0.0	0.009514941304197553	4.066214232563057E-5
20	4.879457079075668E-4	0.0	0.0	0.01598022193397281	8.132428465126114E-5
21	4.879457079075668E-4	0.0	0.0	0.027731581066080046	8.132428465126114E-5
22	4.879457079075668E-4	0.0	0.0	0.04480968084284489	8.132428465126114E-5
23	4.879457079075668E-4	0.0	0.0	0.05408064929308866	8.132428465126114E-5
24	5.286078502331974E-4	0.0	0.0	0.06819041268008247	8.132428465126114E-5
25	5.286078502331974E-4	0.0	0.0	0.0770140975647443	8.132428465126114E-5
26	5.286078502331974E-4	0.0	0.0	0.09209975236755323	8.132428465126114E-5
27	5.286078502331974E-4	0.0	0.0	0.1512631694513457	8.132428465126114E-5
28	5.286078502331974E-4	0.0	0.0	0.2817479841742942	8.132428465126114E-5
29	5.286078502331974E-4	0.0	0.0	0.44220079779123245	8.132428465126114E-5
30	5.286078502331974E-4	0.0	0.0	0.7071959793273669	8.132428465126114E-5
31	5.286078502331974E-4	0.0	0.0	1.027694985137987	8.132428465126114E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9845	0.0	25.011173	1
GTATTGG	755	0.0	21.317883	1
GTACGAT	60	9.240896E-4	18.5	20
ATTGGAC	885	0.0	18.18644	3
CGAACTA	190	0.0	17.526316	24
CGAATTA	170	5.456968E-12	17.411764	15
GGACCCT	1395	0.0	16.97491	6
TTGGACC	1490	0.0	16.513422	4
CGTAACG	90	4.449273E-5	16.444445	21
TGGACCC	1470	0.0	16.234694	5
GTATCAA	15635	0.0	15.772626	2
TATACTG	250	0.0	15.54	5
TCTATAC	240	0.0	15.416667	3
CTGGTCG	110	1.4531277E-5	15.136364	9
TTTTTAC	1680	0.0	14.97619	1
CCGAATT	210	9.094947E-12	14.97619	14
GACCCTC	1515	0.0	14.897689	7
CGGGTAC	75	0.004106175	14.8	6
ATAAGAC	150	8.112511E-8	14.8	3
TATTGGA	1095	0.0	14.698629	2
>>END_MODULE