FastQCFastQC Report
Wed 25 May 2016
SRR2088667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368928
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT359041.515622256142863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT350041.4776303880911534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265721.1216887976333598No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149120.6294830404301017No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG103970.43889050237069255No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG88600.37400883437571764No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA87850.3708428453714085No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA76590.3233107971200475No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG72860.3075652784719502No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA71860.30334395979953804No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC45430.19177450728768455No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45230.19093024355320212No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA44520.1879331072957895No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA43310.18282531170217078No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41760.17628226775993192No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG41510.17522693809182888No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39730.16771299085493524No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA39030.1647580677842467No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37020.15627321725269827No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC32030.1352088370773616No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30700.1295944832430534No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG29570.12482439314322766No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA29390.12406455578219346No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC29020.12250266787340097No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27630.11663503491874806No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT27150.11460880195599023No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT24360.10283132285996029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118100.023.450041
GTATTGG10700.018.8457951
TAATACT4750.017.9157894
GTTCTAT1451.546141E-1017.8620681
CAATGCG1502.5102054E-1017.26666619
ATTGGAC11900.017.2563043
TTGGACC15800.017.0949364
GGACCCT15650.016.6677326
TGGACCC16300.016.0030675
GTATCAA177600.015.6041672
TCTATCG957.064734E-515.57894831
GTACTAT2300.015.2826091
TCTAACG855.367134E-415.2352942
TATTGGA12950.015.0000012
GACCCTC17350.014.6080697
ATACGTC1152.211691E-514.4782615
TATACGT1152.211691E-514.4782614
CGAATTA1356.573542E-613.70370415
ATTAGAC2051.4260877E-913.5365853
CGTTCGC2202.8558134E-1013.45454523
Produced by FastQC (version 0.11.5)