FastQCFastQC Report
Wed 25 May 2016
SRR2088666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030922
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT182701.7722000306521732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT174221.6899435650805785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121521.178750671728802No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67950.6591187306120153No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG46250.44862753923187204No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG43330.42030337891712466No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA43190.4189453712308011No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA36870.3576410242481972No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA33210.32213882330573995No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG33030.320392813423324No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA22570.21893023914515355No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20670.20050013483076315No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19790.19196408651672967No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.19099408102649862No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA18280.17731700361424046No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA18250.17702600196717114No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18100.17557099373182453No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG17760.17227297506503889No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC15360.14899284329949306No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG14860.1441428158483377No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14710.1426878076129911No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.14229980541689866No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14610.14171780212276003No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG14460.14026279388741342No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13910.13492776369114248No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT12830.12445170439664688No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT12530.12154168792595367No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG12060.1169826621218676No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC11530.11184163302364293No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT10330.10020156714087002No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTC250.005496083329.624
GTATTGG3350.022.641791
AGGTCGC1400.021.14285735
GGTATCA64650.021.0610981
AGCATCG450.003825544820.55555519
TCGTGTC1101.7535058E-920.18181834
ATTGGAC3850.019.2207813
GTCGCCC1601.8189894E-1218.537
TATGACC500.00703440118.54
CTGCGCA609.2359364E-418.59
CGTGTCT1205.171387E-918.535
GGACAAT500.00703440118.51
TTGGACC6150.017.1463414
GCGACTA650.001579784417.07692313
CCGAGGT1851.8189894E-1217.00000232
GCTCGGA1851.8189894E-1217.00000211
GTACTGC2200.016.8181826
TATTGGA4450.016.6292152
GGTCGCC1901.8189894E-1216.55263136
TGGACCC6300.016.4444455
Produced by FastQC (version 0.11.5)