##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1030922
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238428319504287	31.0	31.0	33.0	30.0	34.0
2	31.4526957422579	31.0	31.0	34.0	30.0	34.0
3	31.48412683015786	31.0	31.0	34.0	28.0	34.0
4	35.24319783649975	37.0	35.0	37.0	33.0	37.0
5	35.12682724784222	37.0	35.0	37.0	32.0	37.0
6	35.23761545490348	37.0	35.0	37.0	32.0	37.0
7	35.06190866040302	37.0	35.0	37.0	32.0	37.0
8	35.133887917805616	37.0	35.0	37.0	32.0	37.0
9	36.64309714992987	39.0	35.0	39.0	32.0	39.0
10	36.435042612341185	38.0	35.0	39.0	32.0	39.0
11	36.554962451087476	38.0	35.0	39.0	32.0	39.0
12	36.380604934223925	38.0	35.0	39.0	32.0	39.0
13	36.43817185005267	38.0	35.0	39.0	32.0	39.0
14	37.24421731226999	39.0	36.0	40.0	32.0	41.0
15	37.29333548027882	39.0	36.0	40.0	32.0	41.0
16	37.12337208828602	39.0	36.0	40.0	31.0	41.0
17	37.228731174618446	39.0	36.0	40.0	32.0	41.0
18	37.22152403382603	39.0	36.0	40.0	32.0	41.0
19	37.29207932316897	39.0	36.0	40.0	31.0	41.0
20	37.17761673531072	39.0	36.0	40.0	31.0	41.0
21	37.12490857698255	39.0	36.0	40.0	31.0	41.0
22	36.99574555591985	39.0	36.0	40.0	31.0	41.0
23	36.797362943074255	39.0	35.0	40.0	30.0	41.0
24	36.62534507945315	39.0	35.0	40.0	30.0	41.0
25	36.79262446625448	39.0	35.0	40.0	30.0	41.0
26	36.61594087622536	39.0	35.0	40.0	30.0	41.0
27	36.49072286749143	38.0	35.0	40.0	30.0	41.0
28	36.26608996606921	38.0	35.0	40.0	30.0	41.0
29	36.13700648545671	38.0	35.0	40.0	30.0	41.0
30	35.84745596660077	38.0	35.0	40.0	29.0	41.0
31	35.6315860947773	38.0	34.0	40.0	27.0	41.0
32	35.43276406944463	38.0	34.0	40.0	27.0	41.0
33	35.25710868523516	38.0	34.0	40.0	27.0	41.0
34	35.02532587334444	38.0	33.0	40.0	25.0	41.0
35	34.85078114542128	38.0	33.0	40.0	25.0	41.0
36	34.61950952642392	37.0	33.0	40.0	24.0	41.0
37	34.47798863541568	37.0	33.0	40.0	24.0	41.0
38	34.23679386025325	37.0	33.0	40.0	23.0	41.0
39	34.08212745484139	37.0	33.0	40.0	22.0	41.0
40	33.84494753240303	37.0	33.0	40.0	20.0	41.0
41	33.64676280067745	37.0	33.0	40.0	18.0	41.0
42	33.260408643912925	37.0	32.0	40.0	16.0	41.0
43	32.87438816903704	37.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	10.0
16	17.0
17	26.0
18	126.0
19	238.0
20	573.0
21	1173.0
22	2099.0
23	3553.0
24	5713.0
25	8726.0
26	12228.0
27	16697.0
28	22325.0
29	27978.0
30	34867.0
31	41585.0
32	50076.0
33	60045.0
34	72810.0
35	87505.0
36	112301.0
37	150554.0
38	183773.0
39	135920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.12757512207519	22.814335129136833	15.753665165744838	21.30442458304314
2	19.730396674045174	24.778111244109642	34.07959089048444	21.411901191360744
3	21.170078822646136	24.712926875166115	33.451900337755916	20.66509396443184
4	14.59470260601675	17.216336444464275	34.72677855356661	33.46218239595236
5	12.079090367651482	39.818046370142454	34.28785106923705	13.81501219296901
6	32.14035591441448	37.018610525335575	12.40064718766308	18.44038637258687
7	24.67994668849826	34.37941958751487	20.98597178060028	19.9546619433866
8	27.475696512442262	34.937560746593824	18.359099912505506	19.227642828458407
9	23.806068742349083	14.755335515199016	22.60112792238404	38.837467820067864
10	16.914082733708273	28.52107142926429	32.85292194753822	21.711923889489213
11	32.01755321935122	22.473669200967677	23.58752650540002	21.92125107428108
12	20.80244674184856	28.730883616801272	30.919507004409645	19.547162636940524
13	32.956712534992946	20.824465866476803	26.81861479335973	19.400206805170516
14	19.37556866571865	24.288646473739043	29.172527116503478	27.163257744038834
15	22.301784228098732	30.053971105476457	26.831127864183713	20.8131168022411
16	20.410661524344228	28.62573502166022	28.368974568396055	22.594628885599494
17	21.19927598790209	27.142402625998862	27.902304927045883	23.756016459053157
18	22.642450156267884	25.284842112206356	30.958598225665956	21.114109505859805
19	21.913879032555325	24.518731776021852	33.21483099594344	20.352558195479386
20	23.139674970560336	23.74263038328797	32.72216520745507	20.39552943869662
21	21.512781762344773	25.27688806718646	33.85678062937836	19.353549541090402
22	19.715070587299525	25.57613476092275	32.87309806173503	21.8356965900427
23	21.077928301074184	25.67148630061246	34.1352692056237	19.11531619268965
24	21.168526813861767	25.87101642995299	31.256583912264947	21.703872843920298
25	20.820973846711972	26.18035118078768	32.22649240194699	20.772182570553348
26	20.60863964490039	27.381993982085937	32.390714331443114	19.618652041570556
27	19.47800124548705	26.82356182135991	32.036565326959746	21.66187160619329
28	19.854654377343774	26.80454971375138	32.27848469622338	21.062311212681465
29	20.71194522961	25.907876638581772	31.25920292708857	22.12097520471966
30	20.267779715633193	26.57737442793926	33.68654466584281	19.46830119058474
31	21.51569177881547	26.369599251931763	32.869509041421175	19.245199927831592
32	20.876458160753188	25.981015052545196	31.63915407761208	21.503372709089533
33	20.30590093139927	26.349035135538866	32.436401590033	20.908662343028862
34	19.561615718744967	27.13173256560632	33.00948083366152	20.297170881987192
35	18.793371370481957	29.488360904122718	31.752159717224	19.966108008171325
36	21.742867064627585	26.655847872098953	31.538079505530003	20.06320555774346
37	20.524443168348334	27.663586575900016	31.305278187874542	20.506692067877104
38	20.44228370332576	25.674105315436087	33.03140295774074	20.85220802349741
39	19.741842738829902	25.395034735896605	33.64687144129236	21.216251083981135
40	18.414584226546722	26.441961661503004	32.95283251303202	22.190621598918252
41	20.73571036412066	25.086960992199213	32.85486195851868	21.322466685161437
42	18.745744100911608	27.858460678887443	32.12386582108055	21.2719293991204
43	19.694700472004673	27.709467835587947	30.74015298926592	21.85567870314146
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	283.0
1	272.0
2	261.0
3	455.0
4	649.0
5	649.0
6	1247.0
7	1845.0
8	2177.5
9	2510.0
10	3997.5
11	5485.0
12	5485.0
13	11166.5
14	16848.0
15	26905.0
16	36962.0
17	33145.0
18	29328.0
19	29328.0
20	31868.5
21	34409.0
22	26360.5
23	18312.0
24	19140.0
25	19968.0
26	19968.0
27	21461.5
28	22955.0
29	24108.0
30	25261.0
31	27263.0
32	29265.0
33	29265.0
34	32183.5
35	35102.0
36	38796.5
37	42491.0
38	44278.0
39	46065.0
40	46065.0
41	47519.5
42	48974.0
43	52033.5
44	55093.0
45	61273.5
46	67454.0
47	67454.0
48	96322.0
49	125190.0
50	112992.0
51	100794.0
52	89891.5
53	78989.0
54	78989.0
55	70001.5
56	61014.0
57	48117.5
58	35221.0
59	30664.0
60	26107.0
61	26107.0
62	22853.0
63	19599.0
64	17153.5
65	14708.0
66	12460.0
67	10212.0
68	10212.0
69	8809.5
70	7407.0
71	6180.5
72	4954.0
73	4045.5
74	3137.0
75	3137.0
76	2520.5
77	1904.0
78	1448.0
79	992.0
80	761.0
81	530.0
82	530.0
83	425.5
84	321.0
85	245.5
86	170.0
87	130.0
88	90.0
89	90.0
90	62.5
91	35.0
92	25.5
93	16.0
94	12.0
95	8.0
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1030922.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.676259475811804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.38354075480605	43.60625747533097
2	8.827084522642767	9.1230142041402
3	2.5187563914963036	3.904797265299672
4	1.1404461714268632	2.357359690914029
5	0.6538878961543629	1.689524029488277
6	0.43644591470546085	1.3532335401286448
7	0.3151461033987397	1.1399900268417
8	0.2358664495621467	0.9750956679369576
9	0.1778768399651939	0.827280876310093
>10	1.1329320644022545	10.94274789207514
>50	0.09913199959408824	3.582476416660881
>100	0.06337275492786673	6.1324082563743305
>500	0.009458620072017568	3.4223825508621353
>1k	0.005296827240329839	5.6020753080552295
>5k	1.8917240144035138E-4	0.6642603168644694
>10k+	5.675172043210541E-4	4.6770964827172445
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18270	1.7722000306521732	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17422	1.6899435650805785	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12152	1.178750671728802	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6795	0.6591187306120153	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4625	0.44862753923187204	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4333	0.42030337891712466	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4319	0.4189453712308011	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3687	0.3576410242481972	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3321	0.32213882330573995	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3303	0.320392813423324	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2257	0.21893023914515355	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2067	0.20050013483076315	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1979	0.19196408651672967	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1969	0.19099408102649862	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1828	0.17731700361424046	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1825	0.17702600196717114	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1810	0.17557099373182453	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	1776	0.17227297506503889	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1536	0.14899284329949306	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1486	0.1441428158483377	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1471	0.1426878076129911	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1467	0.14229980541689866	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1461	0.14171780212276003	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1446	0.14026279388741342	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1391	0.13492776369114248	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1283	0.12445170439664688	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1253	0.12154168792595367	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	1206	0.1169826621218676	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1153	0.11184163302364293	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1033	0.10020156714087002	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.700054902310747E-5	0.0
8	0.0	0.0	0.0	1.9400109804621493E-4	0.0
9	0.0	0.0	0.0	4.8500274511553736E-4	0.0
10	9.700054902310747E-5	0.0	0.0	7.760043921848597E-4	0.0
11	9.700054902310747E-5	0.0	0.0	9.700054902310747E-4	0.0
12	9.700054902310747E-5	0.0	0.0	0.0017460098824159346	0.0
13	9.700054902310747E-5	0.0	0.0	0.002037011529485257	0.0
14	9.700054902310747E-5	0.0	0.0	0.0025220142746007943	0.0
15	9.700054902310747E-5	0.0	0.0	0.003686020862878084	0.0
16	9.700054902310747E-5	0.0	0.0	0.005238029647247803	0.0
17	9.700054902310747E-5	0.0	0.0	0.00688703898064063	0.0
18	9.700054902310747E-5	0.0	0.0	0.00805104556891792	0.0
19	9.700054902310747E-5	0.0	0.0	0.010185057647426285	0.0
20	9.700054902310747E-5	0.0	0.0	0.0168780955300207	0.0
21	9.700054902310747E-5	0.0	0.0	0.02686915207940077	0.0
22	9.700054902310747E-5	0.0	0.0	0.04365024706039836	0.0
23	9.700054902310747E-5	0.0	0.0	0.05441730800196329	0.0
24	9.700054902310747E-5	0.0	0.0	0.06857938815933698	0.0
25	9.700054902310747E-5	0.0	0.0	0.07692143537532423	0.0
26	9.700054902310747E-5	0.0	0.0	0.09244152321902142	0.0
27	9.700054902310747E-5	0.0	0.0	0.1518058592211632	0.0
28	9.700054902310747E-5	0.0	0.0	0.2728625444020013	0.0
29	9.700054902310747E-5	0.0	0.0	0.4367934722510529	0.0
30	9.700054902310747E-5	0.0	0.0	0.6921959178288949	0.0
31	9.700054902310747E-5	0.0	0.0	0.993770624741736	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGTC	25	0.0054960833	29.6	24
GTATTGG	335	0.0	22.64179	1
AGGTCGC	140	0.0	21.142857	35
GGTATCA	6465	0.0	21.061098	1
AGCATCG	45	0.0038255448	20.555555	19
TCGTGTC	110	1.7535058E-9	20.181818	34
ATTGGAC	385	0.0	19.220781	3
GTCGCCC	160	1.8189894E-12	18.5	37
TATGACC	50	0.007034401	18.5	4
CTGCGCA	60	9.2359364E-4	18.5	9
CGTGTCT	120	5.171387E-9	18.5	35
GGACAAT	50	0.007034401	18.5	1
TTGGACC	615	0.0	17.146341	4
GCGACTA	65	0.0015797844	17.076923	13
CCGAGGT	185	1.8189894E-12	17.000002	32
GCTCGGA	185	1.8189894E-12	17.000002	11
GTACTGC	220	0.0	16.818182	6
TATTGGA	445	0.0	16.629215	2
GGTCGCC	190	1.8189894E-12	16.552631	36
TGGACCC	630	0.0	16.444445	5
>>END_MODULE