FastQCFastQC Report
Wed 25 May 2016
SRR2088665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496508
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT391500.8706756442999768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT388280.86351453172106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT284390.632468573390729No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166260.3697535954567411No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG105290.2341594855385557No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG85010.18905781997941515No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83530.18576637693072046No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA83230.185099192528958No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG68540.1524293963226575No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA66590.14809269771120168No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46860.10421420355529223No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45520.10123411322742004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122050.023.7218361
GTATTGG12650.016.9644261
GTATCAA185200.015.63312
ATTGGAC15050.015.2425243
TTGGACC19700.015.025384
GGACCCT16900.014.6686386
TGGACCC18350.014.3160765
CTAATAC12850.014.2529173
GGTCTAG3350.013.805971
ACGTTCG3400.013.602941522
TAATACT14000.012.954
GACCCTC18100.012.7762437
TAGTACT7300.012.6712334
ACGTTTA2653.274181E-1112.56603826
TTAACGG6050.012.23140435
ATACTGG12300.012.032526
AATACTG13400.012.0111945
TTATGCG5550.012.04
TAACGGC5900.011.91525536
TAATACG2808.367351E-1111.8928584
Produced by FastQC (version 0.11.5)