FastQCFastQC Report
Wed 25 May 2016
SRR2088664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497927
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT369301.055768173549648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT358221.0240922695070538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261400.7472997578279935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154940.44294806609743426No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG95800.27387649885203436No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG77370.2211881494382244No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75410.21558483067256692No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA72480.20720844088512996No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA67940.19422932496876008No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG63360.1811358556081931No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.12258689217928219No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41910.1198138211575027No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA40160.11481085797387997No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39790.11375308861505685No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC39250.11220931711839613No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38370.1096935413460601No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37730.10786388623890665No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG37690.10774953279470956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116150.022.3306061
GTATTGG12700.016.169291
CCGTTTA1851.8189894E-1116.027
TAATACT9600.015.6093744
GTATCAA172350.015.0704962
TTGGACC18300.014.9617484
ACCGTTA2350.014.9574478
GACCGTT2400.014.6458337
GGACCCT17400.014.4597696
GTATTAG7250.014.2896561
ATTGGAC14900.014.1543623
TATACTG4850.013.7319585
CTCTAAT7300.013.6849321
TACCGCA2304.0017767E-1113.6739137
CTAATAC8000.013.6437493
TGTTACG1907.1413524E-913.63157916
CGAACTA5300.013.61320824
AGTACCG3150.013.50793655
AATACTG10550.013.502375
TCGGGTA1652.5994996E-713.45454525
Produced by FastQC (version 0.11.5)