FastQCFastQC Report
Wed 25 May 2016
SRR2088663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235974
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230321.0300656447704668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT218790.9784997499970929No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT152750.6831474784590518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97590.43645409114775036No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG44710.19995760236925833No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA43510.19459081366777967No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG42440.1898054270756279No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG34890.15603938149549146No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA33460.14964395829289606No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.1365400492134524No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.1254486858970632No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA26900.1203055133914795No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.11319451836202031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82150.020.7857591
CGAACTA2300.015.28260924
TAATACT6650.014.7443614
GTATCAA116600.014.5969122
ATACTGG4800.014.2604176
TACTGGT4750.014.0210527
AATACTG5900.013.796615
CTAATAC5150.013.6504853
CTAGCAC2051.4260877E-913.5365863
GTACCGT1102.4579882E-413.4545456
TCTATAC2355.638867E-1113.38297753
TAGCACT2408.185452E-1113.1041674
GGACCCT8550.012.9824566
GCGATAT1000.001833422812.94999915
TCTAACC1307.003581E-512.80769353
GTATTGG5350.012.7943941
TACCCCG2900.012.758625
GTATTAG4550.012.6043951
TCTAATA4700.012.5957442
TAGGGGT2951.8189894E-1212.5423734
Produced by FastQC (version 0.11.5)