##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088663_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2235974
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.570439548939298	31.0	31.0	34.0	30.0	34.0
2	31.7762778100282	31.0	31.0	34.0	30.0	34.0
3	31.848696809533564	31.0	31.0	34.0	30.0	34.0
4	35.567085753233265	37.0	35.0	37.0	33.0	37.0
5	35.4376987388941	37.0	35.0	37.0	33.0	37.0
6	35.49080445479241	37.0	35.0	37.0	33.0	37.0
7	35.35939416111279	37.0	35.0	37.0	33.0	37.0
8	35.38783501060388	37.0	35.0	37.0	33.0	37.0
9	36.989604530285234	39.0	37.0	39.0	33.0	39.0
10	36.807822005085924	39.0	37.0	39.0	32.0	39.0
11	36.89204793973454	39.0	37.0	39.0	33.0	39.0
12	36.76339259758834	39.0	37.0	39.0	32.0	39.0
13	36.801607711002006	39.0	37.0	39.0	32.0	39.0
14	37.810292069585785	40.0	37.0	41.0	32.0	41.0
15	37.82727482519922	40.0	37.0	41.0	32.0	41.0
16	37.70154751352207	40.0	37.0	41.0	32.0	41.0
17	37.7245938459034	39.0	37.0	41.0	32.0	41.0
18	37.687624274700866	39.0	37.0	41.0	32.0	41.0
19	37.73176521730575	40.0	37.0	41.0	32.0	41.0
20	37.61481573578226	39.0	37.0	41.0	32.0	41.0
21	37.55460796950233	39.0	36.0	41.0	32.0	41.0
22	37.41833625972395	39.0	36.0	41.0	32.0	41.0
23	37.25990418493238	39.0	36.0	41.0	31.0	41.0
24	37.10337687289745	39.0	36.0	41.0	31.0	41.0
25	37.287961309031324	39.0	36.0	41.0	31.0	41.0
26	37.161707157596645	39.0	36.0	41.0	31.0	41.0
27	37.03528708294461	39.0	36.0	41.0	31.0	41.0
28	36.86962594377216	39.0	35.0	41.0	30.0	41.0
29	36.76733182049523	39.0	35.0	41.0	30.0	41.0
30	36.577084975048905	39.0	35.0	40.0	30.0	41.0
31	36.44284593649121	39.0	35.0	40.0	30.0	41.0
32	36.26666633869625	38.0	35.0	40.0	30.0	41.0
33	36.13511874467235	38.0	35.0	40.0	30.0	41.0
34	35.95762875596944	38.0	35.0	40.0	29.0	41.0
35	35.82833700212972	38.0	35.0	40.0	28.0	41.0
36	35.63418850129742	38.0	35.0	40.0	27.0	41.0
37	35.569520486374174	38.0	35.0	40.0	26.0	41.0
38	35.36961207956801	38.0	34.0	40.0	25.0	41.0
39	35.28487048597166	38.0	34.0	40.0	25.0	41.0
40	35.13121440589202	38.0	34.0	40.0	24.0	41.0
41	35.00544997392635	38.0	34.0	40.0	24.0	41.0
42	34.754752067778966	38.0	33.0	40.0	23.0	41.0
43	34.404078043841295	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	1.0
15	18.0
16	18.0
17	70.0
18	189.0
19	383.0
20	809.0
21	1724.0
22	3191.0
23	5400.0
24	8687.0
25	13459.0
26	19382.0
27	27062.0
28	36421.0
29	47541.0
30	60094.0
31	74667.0
32	92432.0
33	113183.0
34	139200.0
35	170620.0
36	219395.0
37	301542.0
38	415098.0
39	485382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76796062923809	21.695824727836726	14.117695465152993	23.418519177772193
2	19.056930000080502	23.214223421202572	34.9619449957826	22.766901582934327
3	20.00720938615565	24.65851570724883	32.015309659235754	23.318965247359763
4	14.385542944595958	17.0324878554044	35.53739891429865	33.044570285701
5	13.232935624475061	38.13273320709454	34.680769991064295	13.953561177366106
6	32.426495120247374	36.833388939227376	14.127042622141403	16.613073318383844
7	25.89690220011503	32.79966582795685	22.110588047982667	19.19284392394545
8	26.161976838728897	35.1530921200336	19.347228545591317	19.33770249564619
9	24.505920015170123	14.985728814377985	21.93992416727565	38.56842700317624
10	16.73762753949733	27.883553207684887	33.62789549431254	21.75092375850524
11	32.78311822946063	22.883226728038878	23.14396321245238	21.189691830048112
12	20.570543306854194	27.518119620353364	31.0013891038089	20.909947968983538
13	30.809302791535142	21.31809224973099	26.20866790043176	21.66393705830211
14	20.351667774312222	23.54414675662597	27.960834964986176	28.14335050407563
15	23.1449918469535	29.520736824310124	25.44949091536843	21.884780413367956
16	21.753741322573518	28.31808419954794	27.143383599272617	22.784790878605925
17	21.49009782761338	27.522994453423877	27.52862958156043	23.458278137402313
18	22.35240660222346	26.280717038749106	29.366575818860145	22.000300540167284
19	22.599413052209012	25.767115360017602	30.553530586670508	21.079941001102874
20	23.086359680389844	25.394168268504018	30.32946715838377	21.190004892722367
21	22.044800163150377	26.150885475412505	30.83555533293321	20.968759028503907
22	21.1965344856425	26.501605117054133	30.297892551523407	22.00396784577996
23	21.812552382093887	26.567035215972997	30.92607516903148	20.694337232901635
24	21.886927128848548	26.80907738640968	29.19662750997999	22.107367974761782
25	21.875925212010515	26.821152660988005	29.941224719070973	21.361697407930507
26	21.526502544305075	27.366239500101518	30.015241679912197	21.092016275681203
27	21.03052182181009	27.208634805234766	29.814657952194434	21.946185420760706
28	21.192509394116392	27.099912610790643	29.95110855492953	21.756469440163436
29	21.424936068129593	26.955054039089898	29.617294297697562	22.002715595082947
30	21.453066985573177	27.117488843787985	30.63680525802178	20.79263891261705
31	21.95718733759874	26.929561792757877	30.26511041720521	20.848140452438177
32	21.53334519989946	26.784569051339595	29.824497064813816	21.857588683947128
33	21.152974050682165	26.831886238390968	30.205941571771405	21.809198139155463
34	20.79769263864428	26.854560920654713	30.712119192799204	21.63562724790181
35	20.582842197628416	28.101578998682452	29.9045516629442	21.411027140744928
36	22.02029182808029	26.624370408600456	29.81421071980265	21.541127043516607
37	21.200201791255175	26.86659147199386	29.985724342054066	21.947482394696895
38	21.15623884714223	26.046590881647102	30.912300411364352	21.88486985984631
39	20.6560988634036	25.91765378309408	31.404613828246664	22.02163352525566
40	19.974963930707602	26.346773263016477	31.153627009974176	22.524635796301745
41	20.88289040928025	25.560807057684933	31.24329710452805	22.31300542850677
42	19.848978565940392	27.04065431887848	30.642216769962445	22.46815034521868
43	20.263562993129618	26.815830595525707	30.085144102748956	22.835462308595716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	943.0
1	839.0
2	735.0
3	1364.0
4	1993.0
5	1993.0
6	3028.0
7	4063.0
8	4487.5
9	4912.0
10	7454.0
11	9996.0
12	9996.0
13	18515.0
14	27034.0
15	38659.5
16	50285.0
17	46543.5
18	42802.0
19	42802.0
20	47718.5
21	52635.0
22	43424.5
23	34214.0
24	38586.0
25	42958.0
26	42958.0
27	48005.5
28	53053.0
29	57171.0
30	61289.0
31	68029.5
32	74770.0
33	74770.0
34	83218.5
35	91667.0
36	99351.0
37	107035.0
38	112283.5
39	117532.0
40	117532.0
41	121058.0
42	124584.0
43	131067.0
44	137550.0
45	143850.0
46	150150.0
47	150150.0
48	180713.5
49	211277.0
50	197552.5
51	183828.0
52	167912.5
53	151997.0
54	151997.0
55	139266.0
56	126535.0
57	110737.0
58	94939.0
59	86014.0
60	77089.0
61	77089.0
62	68968.5
63	60848.0
64	53112.0
65	45376.0
66	38861.0
67	32346.0
68	32346.0
69	27646.5
70	22947.0
71	19254.0
72	15561.0
73	12789.5
74	10018.0
75	10018.0
76	7995.0
77	5972.0
78	4705.5
79	3439.0
80	2613.5
81	1788.0
82	1788.0
83	1342.5
84	897.0
85	692.5
86	488.0
87	361.5
88	235.0
89	235.0
90	170.0
91	105.0
92	82.5
93	60.0
94	41.5
95	23.0
96	23.0
97	13.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2235974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.98238722325047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.97132767123912	44.51996892000765
2	11.21204169710064	12.329296363064355
3	3.4265078448964688	5.651927434548093
4	1.4004505443378634	3.0800045646318526
5	0.7284436552533609	2.0025785561730127
6	0.4308869608427624	1.4214716238303797
7	0.29557794670400833	1.1376106784233153
8	0.22907185929766485	1.0075934135883324
9	0.16150103711346905	0.7991741303576373
>10	0.9784333632303372	10.234371205916654
>50	0.09559820660772628	3.6697383601289686
>100	0.06299145372196693	6.550648663644976
>500	0.0041540425271707065	1.576805204804571
>1k	0.002687909870522201	2.8863833751273487
>5k	8.145181425824852E-5	0.4370485385466137
>10k+	2.4435544277474555E-4	2.695378967206321
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23032	1.0300656447704668	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21879	0.9784997499970929	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15275	0.6831474784590518	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9759	0.43645409114775036	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4471	0.19995760236925833	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4351	0.19459081366777967	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4244	0.1898054270756279	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3489	0.15603938149549146	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3346	0.14964395829289606	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3053	0.1365400492134524	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2805	0.1254486858970632	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2690	0.1203055133914795	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2531	0.11319451836202031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.4723239178988666E-5	0.0	4.4723239178988666E-5	0.0
6	0.0	4.4723239178988666E-5	0.0	4.4723239178988666E-5	0.0
7	0.0	4.4723239178988666E-5	0.0	1.34169717536966E-4	0.0
8	0.0	4.4723239178988666E-5	0.0	1.34169717536966E-4	4.4723239178988666E-5
9	0.0	4.4723239178988666E-5	0.0	2.68339435073932E-4	4.4723239178988666E-5
10	4.4723239178988666E-5	4.4723239178988666E-5	0.0	4.02509152610898E-4	4.4723239178988666E-5
11	4.4723239178988666E-5	4.4723239178988666E-5	0.0	6.261253485058413E-4	4.4723239178988666E-5
12	4.4723239178988666E-5	4.4723239178988666E-5	0.0	8.05018305221796E-4	4.4723239178988666E-5
13	4.4723239178988666E-5	4.4723239178988666E-5	0.0	8.944647835797733E-4	4.4723239178988666E-5
14	8.944647835797733E-5	4.4723239178988666E-5	0.0	0.0011180809794747166	4.4723239178988666E-5
15	8.944647835797733E-5	4.4723239178988666E-5	0.0	0.001475866892906626	4.4723239178988666E-5
16	8.944647835797733E-5	4.4723239178988666E-5	0.0	0.0022361619589494333	4.4723239178988666E-5
17	8.944647835797733E-5	4.4723239178988666E-5	0.0	0.0029964570249922406	4.4723239178988666E-5
18	8.944647835797733E-5	4.4723239178988666E-5	0.0	0.0033989661776031386	4.4723239178988666E-5
19	1.7889295671595466E-4	4.4723239178988666E-5	0.0	0.004382877439540889	4.4723239178988666E-5
20	1.7889295671595466E-4	4.4723239178988666E-5	0.0	0.006976825311922232	4.4723239178988666E-5
21	1.7889295671595466E-4	4.4723239178988666E-5	0.0	0.011270256273105144	4.4723239178988666E-5
22	1.7889295671595466E-4	4.4723239178988666E-5	0.0	0.018515421020101307	4.4723239178988666E-5
23	1.7889295671595466E-4	4.4723239178988666E-5	0.0	0.022853575220463208	4.4723239178988666E-5
24	1.7889295671595466E-4	4.4723239178988666E-5	0.0	0.03376604558013644	4.4723239178988666E-5
25	2.2361619589494333E-4	4.4723239178988666E-5	0.0	0.04123482652302755	4.4723239178988666E-5
26	2.68339435073932E-4	4.4723239178988666E-5	0.0	0.05594877221291482	4.4723239178988666E-5
27	2.68339435073932E-4	4.4723239178988666E-5	0.0	0.11726433312730827	4.4723239178988666E-5
28	3.1306267425292066E-4	4.4723239178988666E-5	0.0	0.244636118309068	4.4723239178988666E-5
29	3.1306267425292066E-4	4.4723239178988666E-5	0.0	0.402330259654182	4.4723239178988666E-5
30	3.1306267425292066E-4	4.4723239178988666E-5	0.0	0.6340413618405223	4.4723239178988666E-5
31	3.1306267425292066E-4	4.4723239178988666E-5	0.0	0.9574351043437893	4.4723239178988666E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8215	0.0	20.785759	1
CGAACTA	230	0.0	15.282609	24
TAATACT	665	0.0	14.744361	4
GTATCAA	11660	0.0	14.596912	2
ATACTGG	480	0.0	14.260417	6
TACTGGT	475	0.0	14.021052	7
AATACTG	590	0.0	13.79661	5
CTAATAC	515	0.0	13.650485	3
CTAGCAC	205	1.4260877E-9	13.536586	3
GTACCGT	110	2.4579882E-4	13.454545	6
TCTATAC	235	5.638867E-11	13.3829775	3
TAGCACT	240	8.185452E-11	13.104167	4
GGACCCT	855	0.0	12.982456	6
GCGATAT	100	0.0018334228	12.949999	15
TCTAACC	130	7.003581E-5	12.8076935	3
GTATTGG	535	0.0	12.794394	1
TACCCCG	290	0.0	12.75862	5
GTATTAG	455	0.0	12.604395	1
TCTAATA	470	0.0	12.595744	2
TAGGGGT	295	1.8189894E-12	12.542373	4
>>END_MODULE