FastQCFastQC Report
Wed 25 May 2016
SRR2088662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113499
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT131941.1849135023920094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124721.1200728514349811No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91810.8245180283053689No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52410.47067846491105964No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG33140.2976203840326754No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG29680.26654716349094165No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26900.2415808186626122No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25990.23340838204614464No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23260.20889107219674197No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG22750.20431091541168872No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.13300416075811475No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA13940.12519095212478862No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.12492153113743254No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC13860.12447249615850575No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA13660.1226763562427986No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12670.1137854636600482No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.11198932374434102No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12270.11019318382863388No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG11900.10687032498457566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA405.9399656E-527.759
GGTATCA35650.023.922861
TAACCCG450.003825734120.5555555
AATCGAT450.003825734120.55555522
GTATTGG2950.020.0677971
CGTATAC701.21915364E-418.53
CAAGTCG609.23657E-418.518
CTAATAC2950.018.1864413
ATCTCGT852.7229928E-517.4117647
ATAGACC752.0671939E-417.2666663
GTATACT1401.8681021E-917.1785724
CCGTATA650.001579892417.0769232
CGAATGC650.001579892417.07692323
CTCGTAT904.4457556E-516.4444459
TCTCGTA904.4457556E-516.4444458
ATTGGAC4200.016.297623
GGACCGT1251.6574631E-716.2799996
TTGGACC5300.016.0566044
TGGACCC5300.016.0566045
TAATACT3500.015.8571434
Produced by FastQC (version 0.11.5)