##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113499
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.322945956844148	31.0	31.0	34.0	30.0	34.0
2	31.539885532003172	31.0	31.0	34.0	30.0	34.0
3	31.596613917030908	31.0	31.0	34.0	30.0	34.0
4	35.35561774191086	37.0	35.0	37.0	33.0	37.0
5	35.22988884588132	37.0	35.0	37.0	33.0	37.0
6	35.29629573084484	37.0	35.0	37.0	32.0	37.0
7	35.1512017523141	37.0	35.0	37.0	32.0	37.0
8	35.18786635641343	37.0	35.0	37.0	32.0	37.0
9	36.740023116320714	39.0	35.0	39.0	32.0	39.0
10	36.51541581986154	38.0	35.0	39.0	32.0	39.0
11	36.62751560621069	38.0	35.0	39.0	32.0	39.0
12	36.47235156924254	38.0	35.0	39.0	32.0	39.0
13	36.5221585291051	38.0	35.0	39.0	32.0	39.0
14	37.43361152547061	39.0	36.0	41.0	32.0	41.0
15	37.44928015202528	39.0	36.0	41.0	32.0	41.0
16	37.31076812821565	39.0	36.0	41.0	32.0	41.0
17	37.35858227084174	39.0	36.0	40.0	32.0	41.0
18	37.314298441219975	39.0	36.0	40.0	32.0	41.0
19	37.37900438168332	39.0	36.0	41.0	32.0	41.0
20	37.245487422979274	39.0	36.0	40.0	31.0	41.0
21	37.1813849855276	39.0	36.0	40.0	31.0	41.0
22	37.03847062278457	39.0	36.0	40.0	31.0	41.0
23	36.85952748947238	39.0	36.0	40.0	30.0	41.0
24	36.6996719350444	39.0	35.0	40.0	30.0	41.0
25	36.88236361236068	39.0	36.0	40.0	30.0	41.0
26	36.744974175998365	39.0	35.0	40.0	30.0	41.0
27	36.624900426493426	39.0	35.0	40.0	30.0	41.0
28	36.43869460143206	39.0	35.0	40.0	30.0	41.0
29	36.32735188805738	38.0	35.0	40.0	30.0	41.0
30	36.11852008847785	38.0	35.0	40.0	29.0	41.0
31	35.9573066522736	38.0	35.0	40.0	29.0	41.0
32	35.77850900629457	38.0	35.0	40.0	28.0	41.0
33	35.6391626754941	38.0	34.0	40.0	27.0	41.0
34	35.448354241898734	38.0	34.0	40.0	27.0	41.0
35	35.302600181948975	38.0	34.0	40.0	26.0	41.0
36	35.106035119923774	38.0	34.0	40.0	25.0	41.0
37	35.02715224710575	38.0	34.0	40.0	25.0	41.0
38	34.82462579670031	38.0	33.0	40.0	24.0	41.0
39	34.734823291264746	38.0	33.0	40.0	24.0	41.0
40	34.561088963708094	38.0	33.0	40.0	23.0	41.0
41	34.42153607681731	38.0	33.0	40.0	23.0	41.0
42	34.14420668541238	38.0	33.0	40.0	21.0	41.0
43	33.77495354733143	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	11.0
16	19.0
17	47.0
18	108.0
19	256.0
20	572.0
21	1075.0
22	2003.0
23	3300.0
24	5340.0
25	8110.0
26	11610.0
27	15889.0
28	21527.0
29	27558.0
30	34285.0
31	42549.0
32	51080.0
33	61930.0
34	75390.0
35	91449.0
36	117238.0
37	156885.0
38	208810.0
39	176454.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.169950758824214	22.508417160679983	14.489370893013822	22.832261187481983
2	19.551252403459724	24.03621377298049	34.08534718037466	22.327186643185133
3	20.318922603432963	25.111832161501717	32.435592667797636	22.133652567267685
4	14.327538686608609	17.233693070222785	36.12737865054212	32.31138959262649
5	12.484250098114142	39.2021905722412	34.8812167770245	13.432342552620163
6	32.02140280323557	37.75782465902529	13.67518066922377	16.545591868515373
7	25.635496753926134	33.28319109401984	21.859471809134988	19.221840342919034
8	26.895937939773635	34.77192166315371	19.138230029842866	19.193910367229787
9	24.551077279817942	14.443569325163292	21.893778081524996	39.111575313493766
10	16.214204054067405	28.13024528984759	33.80981931730518	21.845731338779828
11	32.94758235076996	22.676715470781744	23.559338625360237	20.816363553088056
12	20.61339974261315	27.718749635159078	31.11075986597204	20.55709075625573
13	31.287679647669194	21.221033876096882	26.4257085098415	21.065577966392425
14	20.190049564480976	23.305903283253958	28.18080662847475	28.32324052379032
15	22.55709255239565	29.73392881358672	25.80738734385931	21.901591290158322
16	21.41079605819134	28.506806023175596	27.1764051876113	22.905992731021758
17	21.408191655313566	27.305996682529575	27.424182688983105	23.86162897317375
18	22.46962053850071	25.988258633371018	29.839092805651372	21.703028022476893
19	22.4034327826069	25.165626551977144	31.079686645430304	21.35125401998565
20	23.199571800244094	24.966344828329436	30.898456127935454	20.935627243491016
21	22.00702470321033	25.817715148374624	31.4268804911365	20.748379657278544
22	20.85578882423783	26.14515145500804	30.988442737712386	22.010616983041746
23	21.787536405510917	26.295757786940086	31.661186943140496	20.2555188644085
24	21.789332545426625	26.416009354296683	29.485971698223345	22.308686402053347
25	21.719283088714043	26.689292042471525	30.456336287684138	21.135088581130294
26	21.461806431797427	27.37865054211993	30.34964557669113	20.809897449391514
27	20.492788947273414	27.271331182156427	30.118033334560696	22.117846536009463
28	20.868541417639353	27.008106877509547	30.38646644496313	21.736885259887977
29	21.38493164340516	26.656781909997225	29.775419645639555	22.182866800958063
30	21.4815639708702	26.938237034788536	31.120009986537934	20.46018900780333
31	22.082013544691105	26.77245332056877	30.76850540503404	20.377027729706086
32	21.454621872134595	26.65103426226696	30.052653841628956	21.841690023969488
33	21.115331042057512	26.647262368443975	30.61125335541388	21.626153234084626
34	20.4787790559309	27.200024427502857	31.092259624840256	21.22893689172599
35	20.110660180206718	28.93051542929091	30.045110053982988	20.913714336519387
36	22.157092193167664	26.81421357360896	29.87492579696973	21.153768436253646
37	21.190768918517215	27.514528526743177	29.920008908853983	21.37469364588563
38	21.204509388872374	26.1270104418594	30.980809143070626	21.6876710261976
39	20.570382191631964	26.105636376862485	31.72970968092472	21.594271750580827
40	19.653362957667678	26.62499023348921	31.312735799493307	22.408911009349804
41	21.04941270715106	25.599573955611994	31.31507078138373	22.035942555853218
42	19.88290963889505	27.434420686502637	30.638464875136844	22.04420479946547
43	20.31021132484178	27.0586682161367	30.0560665074688	22.575053951552718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	436.0
1	383.0
2	330.0
3	495.0
4	660.0
5	660.0
6	1160.5
7	1661.0
8	1934.0
9	2207.0
10	3397.5
11	4588.0
12	4588.0
13	8848.0
14	13108.0
15	20207.0
16	27306.0
17	25178.0
18	23050.0
19	23050.0
20	25163.5
21	27277.0
22	22244.5
23	17212.0
24	19080.0
25	20948.0
26	20948.0
27	23473.5
28	25999.0
29	28284.5
30	30570.0
31	34009.5
32	37449.0
33	37449.0
34	41542.0
35	45635.0
36	49707.5
37	53780.0
38	56318.0
39	58856.0
40	58856.0
41	60146.5
42	61437.0
43	64563.5
44	67690.0
45	71157.0
46	74624.0
47	74624.0
48	94017.0
49	113410.0
50	105379.5
51	97349.0
52	87908.0
53	78467.0
54	78467.0
55	69893.0
56	61319.0
57	52908.5
58	44498.0
59	39932.0
60	35366.0
61	35366.0
62	31239.5
63	27113.0
64	23553.5
65	19994.0
66	17145.0
67	14296.0
68	14296.0
69	12188.5
70	10081.0
71	8450.0
72	6819.0
73	5551.0
74	4283.0
75	4283.0
76	3429.0
77	2575.0
78	1991.0
79	1407.0
80	1118.0
81	829.0
82	829.0
83	623.0
84	417.0
85	320.0
86	223.0
87	172.5
88	122.0
89	122.0
90	91.5
91	61.0
92	45.5
93	30.0
94	19.5
95	9.0
96	9.0
97	7.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1113499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.40325068293987
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.18617015014037	49.75149249998217
2	8.753442236890674	10.224589625995199
3	2.36163163547795	4.137808932827399
4	1.01116033981616	2.3622020322771937
5	0.5671243398214313	1.6560952493493912
6	0.37804702062566015	1.324750494932338
7	0.2711881218531797	1.1086787503978826
8	0.19525673627080023	0.9122902492764978
9	0.14234359411067116	0.7481995748960553
>10	0.9931145797968407	10.808033188591468
>50	0.08070733511558581	3.2279699823960617
>100	0.05162234247363044	5.5179214663523535
>500	0.004945852140130682	1.8670790236889403
>1k	0.002627483949444425	2.7342724186115688
>5k	3.091157587581676E-4	1.3018281608799989
>10k+	3.091157587581676E-4	2.316788349545559
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13194	1.1849135023920094	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12472	1.1200728514349811	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9181	0.8245180283053689	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5241	0.47067846491105964	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3314	0.2976203840326754	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2968	0.26654716349094165	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2690	0.2415808186626122	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2599	0.23340838204614464	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2326	0.20889107219674197	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2275	0.20431091541168872	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.13300416075811475	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1394	0.12519095212478862	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1391	0.12492153113743254	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1386	0.12447249615850575	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1366	0.1226763562427986	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1267	0.1137854636600482	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1247	0.11198932374434102	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1227	0.11019318382863388	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1190	0.10687032498457566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.980699578535768E-5	0.0
5	0.0	0.0	0.0	8.980699578535768E-5	0.0
6	8.980699578535768E-5	0.0	0.0	8.980699578535768E-5	0.0
7	8.980699578535768E-5	0.0	0.0	8.980699578535768E-5	0.0
8	1.7961399157071536E-4	0.0	0.0	8.980699578535768E-5	0.0
9	2.694209873560731E-4	0.0	0.0	1.7961399157071536E-4	0.0
10	2.694209873560731E-4	0.0	0.0	1.7961399157071536E-4	0.0
11	2.694209873560731E-4	0.0	0.0	5.388419747121461E-4	0.0
12	2.694209873560731E-4	0.0	0.0	7.184559662828615E-4	0.0
13	2.694209873560731E-4	0.0	0.0	7.184559662828615E-4	0.0
14	2.694209873560731E-4	0.0	0.0	7.184559662828615E-4	0.0
15	2.694209873560731E-4	0.0	0.0	0.00116749094520965	0.0
16	2.694209873560731E-4	0.0	0.0	0.0017961399157071537	0.0
17	2.694209873560731E-4	0.0	0.0	0.0024247888862046576	0.0
18	2.694209873560731E-4	0.0	0.0	0.0030534378567021615	0.0
19	2.694209873560731E-4	0.0	0.0	0.004310735797697169	0.0
20	2.694209873560731E-4	0.0	0.0	0.007364173654399331	0.0
21	2.694209873560731E-4	0.0	0.0	0.012752593401520792	0.0
22	2.694209873560731E-4	0.0	0.0	0.02137406499691513	0.0
23	2.694209873560731E-4	0.0	0.0	0.025594993798826942	0.0
24	2.694209873560731E-4	0.0	0.0	0.032689746465870195	0.0
25	2.694209873560731E-4	0.0	0.0	0.03807816621299166	0.0
26	2.694209873560731E-4	0.0	0.0	0.0490346196988053	0.0
27	2.694209873560731E-4	0.0	0.0	0.0952852225282645	0.0
28	2.694209873560731E-4	0.0	0.0	0.20179631952969873	0.0
29	2.694209873560731E-4	0.0	0.0	0.3317470424311113	0.0
30	2.694209873560731E-4	0.0	0.0	0.510193543056617	0.0
31	2.694209873560731E-4	0.0	0.0	0.7283347358192509	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACTA	40	5.9399656E-5	27.75	9
GGTATCA	3565	0.0	23.92286	1
TAACCCG	45	0.0038257341	20.555555	5
AATCGAT	45	0.0038257341	20.555555	22
GTATTGG	295	0.0	20.067797	1
CGTATAC	70	1.21915364E-4	18.5	3
CAAGTCG	60	9.23657E-4	18.5	18
CTAATAC	295	0.0	18.186441	3
ATCTCGT	85	2.7229928E-5	17.411764	7
ATAGACC	75	2.0671939E-4	17.266666	3
GTATACT	140	1.8681021E-9	17.178572	4
CCGTATA	65	0.0015798924	17.076923	2
CGAATGC	65	0.0015798924	17.076923	23
CTCGTAT	90	4.4457556E-5	16.444445	9
TCTCGTA	90	4.4457556E-5	16.444445	8
ATTGGAC	420	0.0	16.29762	3
GGACCGT	125	1.6574631E-7	16.279999	6
TTGGACC	530	0.0	16.056604	4
TGGACCC	530	0.0	16.056604	5
TAATACT	350	0.0	15.857143	4
>>END_MODULE