FastQCFastQC Report
Wed 25 May 2016
SRR2088661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078463
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83320.7725809786705711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80660.747916247474415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64140.5947352853088145No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38170.35392962020950186No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG23670.2194790178244409No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19530.18109105272967177No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA18880.17506395676068628No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG17130.15883715992110994No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA17030.1579099143874199No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG15030.13936500371361837No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG11040.10236790691938434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGT250.005496194629.611
GGTATCA26750.022.6841131
TTAACGG951.6758713E-719.47368435
CTAGACA701.21910845E-418.54
ACACCGT609.23631E-418.56
TATACGT752.0671179E-417.2666664
TAATACT3600.016.9583344
GTATTGG3150.016.4444451
GTATTAG3100.016.1129041
TAACGGC1059.341946E-615.85714436
ATTGGAC3450.015.5507253
TTAATAC2251.8189894E-1214.83
TTAGAGT1001.0932642E-414.84
CGCCACG1001.0932642E-414.818
GAGCGCG750.004104188614.79999911
GTATCAA41050.014.7369072
GTATATA1651.6441845E-814.5757571
TTGGACC4350.014.459774
CCAGTAC1551.2096643E-714.3225813
CAGTACT2201.8189894E-1114.2954544
Produced by FastQC (version 0.11.5)