##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088661_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1078463
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.609249459647664	31.0	31.0	34.0	30.0	34.0
2	31.82434446058882	31.0	31.0	34.0	30.0	34.0
3	31.915974864228073	31.0	31.0	34.0	30.0	34.0
4	35.62212055490082	37.0	35.0	37.0	33.0	37.0
5	35.491644126873155	37.0	35.0	37.0	33.0	37.0
6	35.53122916595191	37.0	35.0	37.0	33.0	37.0
7	35.397231986632825	37.0	35.0	37.0	33.0	37.0
8	35.4193430836292	37.0	35.0	37.0	33.0	37.0
9	37.03320837154357	39.0	37.0	39.0	33.0	39.0
10	36.85085533764255	39.0	37.0	39.0	33.0	39.0
11	36.936744236937194	39.0	37.0	39.0	33.0	39.0
12	36.794958195135116	39.0	37.0	39.0	32.0	39.0
13	36.825210507917284	39.0	37.0	39.0	32.0	39.0
14	37.86016951902847	40.0	37.0	41.0	32.0	41.0
15	37.86457764429563	40.0	37.0	41.0	32.0	41.0
16	37.76646023090268	40.0	37.0	41.0	32.0	41.0
17	37.75640332584428	40.0	37.0	41.0	32.0	41.0
18	37.701945268405126	39.0	37.0	41.0	32.0	41.0
19	37.737860269661546	40.0	37.0	41.0	32.0	41.0
20	37.61043262494865	39.0	37.0	41.0	32.0	41.0
21	37.5415939165275	39.0	36.0	41.0	32.0	41.0
22	37.4076291908021	39.0	36.0	41.0	32.0	41.0
23	37.23251701727366	39.0	36.0	41.0	31.0	41.0
24	37.07335903039789	39.0	36.0	41.0	31.0	41.0
25	37.27297644889069	39.0	36.0	41.0	31.0	41.0
26	37.1682579745434	39.0	36.0	41.0	31.0	41.0
27	37.065017529576814	39.0	36.0	41.0	31.0	41.0
28	36.908994559850456	39.0	36.0	41.0	30.0	41.0
29	36.82009674879899	39.0	35.0	41.0	30.0	41.0
30	36.65474939798584	39.0	35.0	40.0	30.0	41.0
31	36.52328452621926	39.0	35.0	40.0	30.0	41.0
32	36.35927426346569	39.0	35.0	40.0	30.0	41.0
33	36.23243820140329	38.0	35.0	40.0	30.0	41.0
34	36.05023630852426	38.0	35.0	40.0	29.0	41.0
35	35.936934322271604	38.0	35.0	40.0	28.0	41.0
36	35.76041737175962	38.0	35.0	40.0	27.0	41.0
37	35.7212764832915	38.0	35.0	40.0	27.0	41.0
38	35.551782490451686	38.0	34.0	40.0	26.0	41.0
39	35.47370656202392	38.0	34.0	40.0	26.0	41.0
40	35.344310375043	38.0	34.0	40.0	25.0	41.0
41	35.240856663603665	38.0	34.0	40.0	25.0	41.0
42	35.02073691911544	38.0	34.0	40.0	24.0	41.0
43	34.69659227993913	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	8.0
16	13.0
17	36.0
18	71.0
19	182.0
20	361.0
21	697.0
22	1380.0
23	2466.0
24	4003.0
25	5940.0
26	8893.0
27	12431.0
28	16715.0
29	22267.0
30	28455.0
31	35470.0
32	43621.0
33	53458.0
34	66400.0
35	82119.0
36	105418.0
37	144552.0
38	203190.0
39	240313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45427335012884	21.175320803773516	13.163733943584527	24.206671902513115
2	18.770324063041567	22.891930460293956	34.99211377673597	23.34563169992851
3	19.707027501175283	24.734181886629397	31.77670443955889	23.782086172636426
4	13.921571718269426	16.26555570288457	36.76853077017942	33.044341808666594
5	12.989782681464268	38.18897820323924	35.25443153821689	13.566807577079604
6	32.55392164589791	37.28741737083238	14.405501162302276	15.753159820967433
7	26.51096977828632	32.31775220846705	22.383892632385162	18.787385380861465
8	26.557146605864084	34.75761338126574	19.61105758843836	19.074182424431807
9	24.871043327402052	14.487747841140585	21.65702485852551	38.984183972931845
10	15.942596083500316	27.436360820909016	34.50864795546996	22.112395140120707
11	33.24898489795199	22.55515488245772	23.62501077922933	20.57084944036096
12	20.667375700418095	26.661369003850847	31.50743233657529	21.163822959155763
13	30.565629048006283	21.13489289850463	26.32496432422809	21.974513729260995
14	20.690649563313716	22.504063653551395	27.53492702114027	29.27035976199462
15	22.95572495301183	29.40907569383465	25.088296955945637	22.546902397207877
16	22.226168167104483	28.390589199629474	26.294643395276424	23.08859923798962
17	21.66295922994113	27.09559808727791	27.230419587876447	24.01102309490451
18	22.769719498953602	26.224450908376085	28.909753973942546	22.096075618727763
19	22.67857126299187	25.597818376708336	29.804731363060206	21.91887899723959
20	23.538220597275938	25.3337388487134	29.51580165476238	21.612238899248283
21	22.645190423779027	26.021291411944592	29.85146453795819	21.482053626318194
22	21.69290926067932	26.281012885931183	29.468419407990815	22.557658445398683
23	22.34875002665831	26.333031360371194	30.042477117898343	21.275741495072154
24	22.420240657305815	26.429650344981702	28.393370936230543	22.756738061481943
25	22.388714309160353	26.593031008017892	29.162335657319723	21.855919025502036
26	22.24156044296374	26.978579700926225	29.006280233999682	21.773579622110358
27	21.406112217108976	27.241082911513885	28.848184870505527	22.50462000087161
28	21.785819263155066	26.632624392306457	29.0914940985458	22.490062245992675
29	21.840990372409625	26.74936460499804	28.853099271834083	22.556545750758257
30	22.016147053723678	26.898280237708665	29.61650051972112	21.469072188846532
31	22.58890661988404	26.560948312552213	29.54389719443319	21.30624787313056
32	22.140768853451625	26.561133761658954	28.9012233150326	22.396874069856825
33	21.965241273924093	26.57244615716997	29.325623595802547	22.13668897310339
34	21.371618683255708	26.794428737935377	29.748169385505115	22.085783193303804
35	21.284921225855687	28.108521108280954	28.966686849711117	21.639870816152246
36	22.561367427533444	26.64171139853662	28.801451695607543	21.99546947832239
37	21.869920433060756	26.8242860441202	29.06284221155478	22.24295131126427
38	21.84126854606973	26.072197191744177	29.697449054812264	22.389085207373828
39	21.394243474277747	25.847247425270965	30.446477996927108	22.312031103524184
40	20.74637701988849	26.22686174676368	30.215501134484914	22.811260098862917
41	21.571069197552443	25.43462316277888	30.16283358817131	22.831474051497363
42	20.574836596155826	26.849507122636567	29.77487405687539	22.800782224332224
43	21.02380888356856	26.543145198305368	29.32627266767613	23.106773250449947
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	386.0
1	345.5
2	305.0
3	553.5
4	802.0
5	802.0
6	1256.5
7	1711.0
8	1833.0
9	1955.0
10	2846.0
11	3737.0
12	3737.0
13	6751.5
14	9766.0
15	14201.5
16	18637.0
17	17907.0
18	17177.0
19	17177.0
20	18984.0
21	20791.0
22	18072.5
23	15354.0
24	17586.0
25	19818.0
26	19818.0
27	22276.5
28	24735.0
29	27185.5
30	29636.0
31	33161.0
32	36686.0
33	36686.0
34	40752.0
35	44818.0
36	48140.0
37	51462.0
38	54711.5
39	57961.0
40	57961.0
41	59822.0
42	61683.0
43	64134.5
44	66586.0
45	69839.5
46	73093.0
47	73093.0
48	86738.5
49	100384.0
50	95558.5
51	90733.0
52	83882.0
53	77031.0
54	77031.0
55	70689.0
56	64347.0
57	56638.5
58	48930.0
59	44445.0
60	39960.0
61	39960.0
62	35329.0
63	30698.0
64	26795.5
65	22893.0
66	19532.0
67	16171.0
68	16171.0
69	13684.0
70	11197.0
71	9403.5
72	7610.0
73	6232.5
74	4855.0
75	4855.0
76	3875.0
77	2895.0
78	2273.0
79	1651.0
80	1312.5
81	974.0
82	974.0
83	738.5
84	503.0
85	380.0
86	257.0
87	195.0
88	133.0
89	133.0
90	113.5
91	94.0
92	62.5
93	31.0
94	20.5
95	10.0
96	10.0
97	8.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1078463.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.02252292350773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.00565527618343	53.57270858271066
2	8.874138532665853	11.18541198202634
3	2.398959013106178	4.535653481881187
4	1.0560140412227392	2.662106764820245
5	0.6424866709373143	2.024556547359753
6	0.40358512647083394	1.526097173075694
7	0.27569254383813335	1.2162387764715241
8	0.20917179903567948	1.054602759974197
9	0.15464498247494787	0.8771505257729569
>10	0.8866920023630671	10.166376538217182
>50	0.05956963822742529	2.601376544851793
>100	0.029105769348971803	3.6218949019383775
>500	0.0023639195174775898	1.1389534147837266
>1k	0.0014774496984234936	1.692787204117736
>5k	4.43234909527048E-4	2.1240848019985585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8332	0.7725809786705711	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8066	0.747916247474415	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6414	0.5947352853088145	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3817	0.35392962020950186	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2367	0.2194790178244409	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1953	0.18109105272967177	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1888	0.17506395676068628	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1713	0.15883715992110994	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1703	0.1579099143874199	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1503	0.13936500371361837	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1104	0.10236790691938434	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.272455336900756E-5	0.0
10	0.0	0.0	0.0	1.8544910673801512E-4	0.0
11	0.0	0.0	0.0	5.563473202140454E-4	0.0
12	0.0	0.0	0.0	9.272455336900756E-4	0.0
13	0.0	0.0	0.0	0.001019970087059083	0.0
14	0.0	0.0	0.0	0.001483592853904121	0.0
15	0.0	0.0	0.0	0.0017617665140111437	0.0
16	0.0	0.0	0.0	0.002318113834225189	0.0
17	0.0	0.0	0.0	0.00343080847465328	0.0
18	0.0	0.0	0.0	0.0038944312414983177	0.0
19	0.0	0.0	0.0	0.004914401328557401	0.0
20	0.0	0.0	0.0	0.006768892395937552	0.0
21	0.0	0.0	0.0	0.010199700870590831	0.0
22	0.0	0.0	0.0	0.01678314415979037	0.0
23	0.0	0.0	0.0	0.021326647274871737	0.0
24	0.0	0.0	0.0	0.030877276271879518	0.0
25	0.0	0.0	0.0	0.03773889322118608	0.0
26	0.0	0.0	0.0	0.04608410302439676	0.0
27	0.0	0.0	0.0	0.08651200829328405	0.0
28	0.0	0.0	0.0	0.17608392684774535	0.0
29	0.0	0.0	9.272455336900756E-5	0.28271716322210405	0.0
30	0.0	0.0	9.272455336900756E-5	0.4302419276321951	0.0
31	0.0	0.0	9.272455336900756E-5	0.6226453758728857	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGT	25	0.0054961946	29.6	11
GGTATCA	2675	0.0	22.684113	1
TTAACGG	95	1.6758713E-7	19.473684	35
CTAGACA	70	1.21910845E-4	18.5	4
ACACCGT	60	9.23631E-4	18.5	6
TATACGT	75	2.0671179E-4	17.266666	4
TAATACT	360	0.0	16.958334	4
GTATTGG	315	0.0	16.444445	1
GTATTAG	310	0.0	16.112904	1
TAACGGC	105	9.341946E-6	15.857144	36
ATTGGAC	345	0.0	15.550725	3
TTAATAC	225	1.8189894E-12	14.8	3
TTAGAGT	100	1.0932642E-4	14.8	4
CGCCACG	100	1.0932642E-4	14.8	18
GAGCGCG	75	0.0041041886	14.799999	11
GTATCAA	4105	0.0	14.736907	2
GTATATA	165	1.6441845E-8	14.575757	1
TTGGACC	435	0.0	14.45977	4
CCAGTAC	155	1.2096643E-7	14.322581	3
CAGTACT	220	1.8189894E-11	14.295454	4
>>END_MODULE