FastQCFastQC Report
Wed 25 May 2016
SRR2088660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615302
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT189881.1755077378719274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT165491.0245143013504594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107430.6650768710742635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.4430131331478572No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG38330.2372930882274646No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32290.19990069968340288No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA31480.1948861575111032No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA29860.18485707316650385No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA26690.16523225997367674No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG21370.13229724224943695No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20390.1262302653002349No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.1093294009417434No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16260.10066229101431189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA1002.0008883E-1124.0526
GGTATCA54950.021.3111921
TCACGTT1258.58563E-917.76000224
CAATCGT700.00259310415.85714214
CTAATAC3400.015.7794123
GTATTGG4400.015.5568181
GTATCAA76100.015.2910642
ACGTTCG1853.0559022E-1015.00000122
TTGGACC6950.014.9064754
TACCGTC1252.960358E-614.8000017
CGTTTAT1508.10669E-814.827
ATTGGAC5200.014.5865383
ACGACTT1152.2108305E-514.4782615
CAGTTCG2059.276846E-1114.43902413
TTCGTTA1803.3323886E-914.38888830
CGTTCGC1803.3323886E-914.38888823
GGACCGA908.2765747E-414.3888886
TATACTG2600.014.2307695
CTGTGCG1051.6562863E-414.0952399
TAATACT3950.014.0506324
Produced by FastQC (version 0.11.5)