##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1615302
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.016847623540365	31.0	31.0	33.0	30.0	34.0
2	31.237581579172193	31.0	31.0	34.0	28.0	34.0
3	31.273329693147165	31.0	31.0	34.0	28.0	34.0
4	35.05885896259647	35.0	35.0	37.0	32.0	37.0
5	34.89693815769435	35.0	35.0	37.0	32.0	37.0
6	35.00947810378493	36.0	35.0	37.0	32.0	37.0
7	34.874857456995656	35.0	35.0	37.0	32.0	37.0
8	34.91240151996345	36.0	35.0	37.0	32.0	37.0
9	36.42918661649648	38.0	35.0	39.0	32.0	39.0
10	36.13738731209396	38.0	35.0	39.0	32.0	39.0
11	36.299768711980796	38.0	35.0	39.0	32.0	39.0
12	36.09836736412139	38.0	35.0	39.0	31.0	39.0
13	36.169309516115256	38.0	35.0	39.0	32.0	39.0
14	37.004134830514666	39.0	36.0	40.0	31.0	41.0
15	37.03415150851048	39.0	36.0	40.0	31.0	41.0
16	36.87460115817352	38.0	36.0	40.0	31.0	41.0
17	36.90384212983083	38.0	36.0	40.0	31.0	41.0
18	36.85315191834097	38.0	36.0	40.0	31.0	41.0
19	36.91387554773039	39.0	36.0	40.0	31.0	41.0
20	36.7754017514991	39.0	36.0	40.0	30.0	41.0
21	36.69738104701164	39.0	35.0	40.0	30.0	41.0
22	36.536582013765845	38.0	35.0	40.0	30.0	41.0
23	36.34123526127003	38.0	35.0	40.0	30.0	41.0
24	36.17211704065246	38.0	34.0	40.0	29.0	41.0
25	36.36565608164913	38.0	35.0	40.0	30.0	41.0
26	36.195991833106135	38.0	35.0	40.0	30.0	41.0
27	36.05251587628815	38.0	35.0	40.0	29.0	41.0
28	35.857174695505854	38.0	34.0	40.0	28.0	41.0
29	35.74168174124715	38.0	34.0	40.0	27.0	41.0
30	35.52529310308537	38.0	34.0	40.0	27.0	41.0
31	35.37571983443344	38.0	34.0	40.0	27.0	41.0
32	35.185967701395775	38.0	34.0	40.0	26.0	41.0
33	35.041681369799576	38.0	33.0	40.0	26.0	41.0
34	34.83368311312683	38.0	33.0	40.0	25.0	41.0
35	34.67060586812868	37.0	33.0	40.0	25.0	41.0
36	34.47311214868799	37.0	33.0	40.0	24.0	41.0
37	34.42109463122066	37.0	33.0	40.0	24.0	41.0
38	34.20239435102538	37.0	33.0	40.0	23.0	41.0
39	34.0906400165418	37.0	33.0	40.0	23.0	41.0
40	33.913448383026825	37.0	33.0	40.0	21.0	41.0
41	33.782798510742886	37.0	32.0	40.0	21.0	41.0
42	33.511587925972975	37.0	32.0	40.0	19.0	41.0
43	33.14099221074449	37.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	4.0
13	3.0
14	4.0
15	25.0
16	40.0
17	114.0
18	267.0
19	631.0
20	1238.0
21	2290.0
22	4033.0
23	6709.0
24	10164.0
25	14926.0
26	20999.0
27	27982.0
28	36463.0
29	46331.0
30	57433.0
31	69125.0
32	83223.0
33	99173.0
34	118717.0
35	143677.0
36	183539.0
37	238449.0
38	292340.0
39	157402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.302248124499314	23.950815389320386	14.85753128517144	21.889405201008852
2	21.004493277418092	25.189097766238138	32.264369139640756	21.542039816703006
3	21.976881103347857	25.386150701231102	31.560104550108896	21.076863645312148
4	16.014652368411603	18.307783931425824	34.87960765231517	30.797956047847396
5	12.859948170682634	39.78667766151469	34.2645523871078	13.088821780694879
6	32.37202702652507	38.18734824819136	13.561612627236268	15.879012098047301
7	25.903329532186554	33.30838443832794	21.75809848560826	19.030187543877243
8	27.28845751444621	35.100557047536626	18.949088158127704	18.66189727988946
9	24.801925584194162	14.27664919624937	21.58450865534742	39.33691656420905
10	16.25070729807801	28.60542486791944	33.77151764809305	21.372350185909507
11	33.73653966874306	22.68888418388636	23.075684918362015	20.49889122900857
12	20.242654314796862	27.797897854395032	31.3000293443579	20.659418486450214
13	31.534103220326603	21.12601854018629	26.11709760775384	21.22278063173326
14	20.173069803665197	23.065779649873523	28.04491048732683	28.716240059134453
15	23.15313173635642	30.057475320404482	24.99928805882739	21.790104884411708
16	21.67198455768643	28.550760167448562	26.88617979795729	22.891075476907723
17	21.651678757285016	27.505135262632002	27.679901343525852	23.163284636557126
18	22.34269505021352	26.325170153940253	29.508599630285854	21.823535165560372
19	22.57045431752081	25.60078548779114	30.735676672226	21.093083522462052
20	23.252370145025512	25.370302271649514	30.319407763997074	21.057919819327903
21	22.266053035283804	26.157709208556668	30.735181408801576	20.841056347357956
22	21.37445505546331	26.49015478220172	30.350609359735824	21.784780802599144
23	22.011735266841743	26.589269375014702	30.86537378149721	20.53362157664635
24	22.007339803949975	26.689560218460695	29.125822911133646	22.177277066455684
25	22.00504921061201	26.75047761966493	29.905862804602485	21.338610365120577
26	21.795676597936485	27.38534342184929	29.89156207322222	20.927417906992005
27	21.28982691781475	27.14693599091687	29.70825269825704	21.854984393011335
28	21.332667204027484	27.20153878345969	29.995505484423347	21.470288528089483
29	21.713772409122257	26.75190150201015	29.575212560870973	21.95911352799662
30	21.6674033710105	27.082737469525824	30.492068975337123	20.75779018412656
31	22.11512150669039	26.94969733213975	30.328632045276983	20.606549115892882
32	21.655764680536517	26.82860542486792	29.655878591124136	21.859751303471427
33	21.496227949943723	26.885003547324278	30.043669852448645	21.57509865028335
34	21.09989339454789	27.249641243556933	30.438890065139525	21.21157529675565
35	20.741013135624176	28.465017687095045	29.79529524509968	20.998673932181102
36	22.203030764525767	26.924315081637985	29.572860059604956	21.299794094231295
37	21.507247561137174	27.255089141225604	29.680579854417317	21.557083443219906
38	21.49529933102293	26.194792057460464	30.629318851830806	21.680589759685805
39	20.906493027310063	26.146875321147377	31.12662523788121	21.820006413661346
40	20.182108361160946	26.64504841819053	30.86766437483517	22.30517884581335
41	21.134747558041777	25.699219093395538	30.933224870643382	22.2328084779193
42	20.25967899501146	27.166003632757217	30.265424050734786	22.308893321496537
43	20.51597781715122	26.91354310215675	29.884814109064433	22.6856649716276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	538.0
1	464.0
2	390.0
3	579.5
4	769.0
5	769.0
6	1471.0
7	2173.0
8	2550.0
9	2927.0
10	4427.0
11	5927.0
12	5927.0
13	12088.5
14	18250.0
15	28790.5
16	39331.0
17	35473.0
18	31615.0
19	31615.0
20	35719.5
21	39824.0
22	33074.5
23	26325.0
24	29151.0
25	31977.0
26	31977.0
27	35725.0
28	39473.0
29	42886.5
30	46300.0
31	50862.5
32	55425.0
33	55425.0
34	61472.0
35	67519.0
36	72633.0
37	77747.0
38	81552.5
39	85358.0
40	85358.0
41	88033.0
42	90708.0
43	94302.5
44	97897.0
45	101806.0
46	105715.0
47	105715.0
48	127061.5
49	148408.0
50	139563.0
51	130718.0
52	120396.5
53	110075.0
54	110075.0
55	100391.5
56	90708.0
57	79522.0
58	68336.0
59	61734.0
60	55132.0
61	55132.0
62	49222.0
63	43312.0
64	38126.0
65	32940.0
66	28420.0
67	23900.0
68	23900.0
69	20338.0
70	16776.0
71	14194.0
72	11612.0
73	9592.0
74	7572.0
75	7572.0
76	6030.5
77	4489.0
78	3466.0
79	2443.0
80	1882.0
81	1321.0
82	1321.0
83	1014.5
84	708.0
85	533.5
86	359.0
87	259.5
88	160.0
89	160.0
90	117.0
91	74.0
92	57.5
93	41.0
94	29.0
95	17.0
96	17.0
97	13.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1615302.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.75604319777502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.12349423216344	51.71766692714874
2	9.058777955037723	11.007510095106477
3	2.4299729628529043	4.429066269015493
4	1.011073834088042	2.4571538215997237
5	0.5432803681680458	1.6503782758460452
6	0.3327700364675118	1.213067442632719
7	0.22839958206152255	0.9713658411858539
8	0.17174611892387595	0.834769169631137
9	0.13212768691739182	0.7224799908577636
>10	0.8408716443303746	9.670600165854818
>50	0.0768785776925949	3.250185915114387
>100	0.04467748817378339	5.022298118309776
>500	0.0034759214857536525	1.4480958713874585
>1k	0.002044659697502149	2.2863069799219766
>5k	1.0223298487510743E-4	0.44447859893834835
>10k+	3.066989546253223E-4	2.87457651744924
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18988	1.1755077378719274	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16549	1.0245143013504594	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10743	0.6650768710742635	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7156	0.4430131331478572	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3833	0.2372930882274646	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3229	0.19990069968340288	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3148	0.1948861575111032	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2986	0.18485707316650385	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2669	0.16523225997367674	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2137	0.13229724224943695	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2039	0.1262302653002349	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1766	0.1093294009417434	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1626	0.10066229101431189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2381585610616466E-4	0.0
10	6.190792805308233E-5	0.0	0.0	2.476317122123293E-4	0.0
11	6.190792805308233E-5	0.0	0.0	3.0953964026541166E-4	0.0
12	6.190792805308233E-5	0.0	0.0	5.57171352477741E-4	0.0
13	6.190792805308233E-5	0.0	0.0	6.190792805308233E-4	0.0
14	6.190792805308233E-5	0.0	0.0	6.809872085839057E-4	0.0
15	6.190792805308233E-5	0.0	0.0	8.048030646900703E-4	0.0
16	6.190792805308233E-5	0.0	0.0	0.0013619744171678113	0.0
17	6.190792805308233E-5	0.0	0.0	0.0022905933379640466	0.0
18	6.190792805308233E-5	0.0	0.0	0.0025382250501763755	0.0
19	6.190792805308233E-5	0.0	0.0	0.0032192122587602814	0.0
20	6.190792805308233E-5	0.0	0.0	0.00476691046008734	0.0
21	6.190792805308233E-5	0.0	0.0	0.00798612271884762	0.0
22	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.013186388675306537	0.0
23	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.01721040399875689	0.0
24	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.02358692058822437	0.0
25	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.02816810726415246	0.0
26	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.0371447568318494	0.0
27	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.08153274124590944	0.0
28	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.17309456683641822	0.0
29	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.27877140002302975	0.0
30	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.42957911276033833	0.0
31	1.2381585610616466E-4	6.190792805308233E-5	0.0	0.6209984263004689	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTA	100	2.0008883E-11	24.05	26
GGTATCA	5495	0.0	21.311192	1
TCACGTT	125	8.58563E-9	17.760002	24
CAATCGT	70	0.002593104	15.857142	14
CTAATAC	340	0.0	15.779412	3
GTATTGG	440	0.0	15.556818	1
GTATCAA	7610	0.0	15.291064	2
ACGTTCG	185	3.0559022E-10	15.000001	22
TTGGACC	695	0.0	14.906475	4
TACCGTC	125	2.960358E-6	14.800001	7
CGTTTAT	150	8.10669E-8	14.8	27
ATTGGAC	520	0.0	14.586538	3
ACGACTT	115	2.2108305E-5	14.478261	5
CAGTTCG	205	9.276846E-11	14.439024	13
TTCGTTA	180	3.3323886E-9	14.388888	30
CGTTCGC	180	3.3323886E-9	14.388888	23
GGACCGA	90	8.2765747E-4	14.388888	6
TATACTG	260	0.0	14.230769	5
CTGTGCG	105	1.6562863E-4	14.095239	9
TAATACT	395	0.0	14.050632	4
>>END_MODULE