FastQCFastQC Report
Wed 25 May 2016
SRR2088659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4143193
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT396060.9559294003441308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT393590.9499678146781962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT292800.7067013291439719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168370.4063774002321398No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG116200.2804600220168358No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG89240.21538943515303297No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA88310.213144789537924No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA85940.20742456361554965No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA78640.18980530233566237No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG73830.1781958986704216No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC49880.12039024008777771No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49810.12022128826728565No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48720.11759046706248057No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG47350.11428383857570719No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA46220.11155647347347807No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.11129097775556196No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44960.10851534070462081No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.10446049701281113No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA41470.10009188565437332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131150.022.0053371
GTATTGG14350.019.595821
TGGACCC21200.017.1037735
ACCGATC1201.0430995E-716.9583348
TTGGACC21350.016.6370034
ATTGGAC17250.016.1942043
GGACCCT22000.016.1454546
CTAATAC10850.015.51612953
GTATCAA193500.014.9338492
GACCCTC22000.014.7999997
TAATACT11500.014.6391314
GTATTAG10650.014.417841
CGAACTA5700.013.95614124
TTAACGG5150.013.29126235
TATACTG6550.013.2748095
TCTAATA10500.012.8619042
TATTGGA21800.012.6444952
CCCTCGC25150.012.504979
GGGTAAG5550.012.3333341
CGTACAC1205.1264383E-412.3333343
Produced by FastQC (version 0.11.5)