##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088659_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4143193
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.566170342535333	31.0	31.0	34.0	30.0	34.0
2	31.77413627605569	31.0	31.0	34.0	30.0	34.0
3	31.85683650266835	31.0	31.0	34.0	30.0	34.0
4	35.56636294761069	37.0	35.0	37.0	33.0	37.0
5	35.44277155324408	37.0	35.0	37.0	33.0	37.0
6	35.488351616736175	37.0	35.0	37.0	33.0	37.0
7	35.34988522137395	37.0	35.0	37.0	33.0	37.0
8	35.375493490165674	37.0	35.0	37.0	33.0	37.0
9	36.9746118512944	39.0	37.0	39.0	33.0	39.0
10	36.79144152830921	39.0	37.0	39.0	32.0	39.0
11	36.879189069879196	39.0	37.0	39.0	33.0	39.0
12	36.74218024600833	39.0	35.0	39.0	32.0	39.0
13	36.77449783295154	39.0	37.0	39.0	32.0	39.0
14	37.78018113083315	40.0	37.0	41.0	32.0	41.0
15	37.79161627276354	40.0	37.0	41.0	32.0	41.0
16	37.67820205334388	39.0	37.0	41.0	32.0	41.0
17	37.70004752373351	39.0	37.0	41.0	32.0	41.0
18	37.65599816373507	39.0	37.0	41.0	32.0	41.0
19	37.70250239368526	40.0	37.0	41.0	32.0	41.0
20	37.574238033323574	39.0	37.0	41.0	32.0	41.0
21	37.507961613180946	39.0	36.0	41.0	32.0	41.0
22	37.377544323906704	39.0	36.0	41.0	31.0	41.0
23	37.203678419035754	39.0	36.0	41.0	31.0	41.0
24	37.046539468472744	39.0	36.0	41.0	31.0	41.0
25	37.234059094036894	39.0	36.0	41.0	31.0	41.0
26	37.11078557045255	39.0	36.0	41.0	31.0	41.0
27	37.00222147507973	39.0	36.0	41.0	31.0	41.0
28	36.831952795826794	39.0	35.0	41.0	30.0	41.0
29	36.72787075089188	39.0	35.0	41.0	30.0	41.0
30	36.54186782995627	39.0	35.0	40.0	30.0	41.0
31	36.4054945062902	39.0	35.0	40.0	30.0	41.0
32	36.234926058235764	38.0	35.0	40.0	30.0	41.0
33	36.105840109307	38.0	35.0	40.0	30.0	41.0
34	35.92181247651268	38.0	35.0	40.0	29.0	41.0
35	35.78880805214722	38.0	35.0	40.0	28.0	41.0
36	35.60459698594779	38.0	35.0	40.0	27.0	41.0
37	35.54379339799039	38.0	34.0	40.0	26.0	41.0
38	35.359490856448154	38.0	34.0	40.0	25.0	41.0
39	35.28348474232313	38.0	34.0	40.0	25.0	41.0
40	35.12986529954072	38.0	34.0	40.0	24.0	41.0
41	35.01210757017595	38.0	34.0	40.0	24.0	41.0
42	34.7633832167606	38.0	33.0	40.0	23.0	41.0
43	34.429432082937	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	6.0
14	6.0
15	29.0
16	54.0
17	95.0
18	327.0
19	663.0
20	1578.0
21	3126.0
22	5810.0
23	9945.0
24	16595.0
25	25432.0
26	36709.0
27	51498.0
28	69275.0
29	89790.0
30	112914.0
31	140164.0
32	172144.0
33	210402.0
34	257778.0
35	315461.0
36	404692.0
37	558171.0
38	764052.0
39	896474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52200971569512	21.243929500749783	13.507987680033251	23.72607310352185
2	18.948960379108577	23.119391252109185	34.88082741981848	23.050820948963757
3	19.88734292609589	24.686371115224418	32.09283275000706	23.333453208672637
4	13.891170408909264	16.4685787024645	36.35642848402186	33.28382240460437
5	12.919262993541455	38.29872274837305	35.05434093946384	13.727673318621653
6	32.49061774336846	37.0531616557568	13.988559065435762	16.46766153543897
7	26.241234719212937	32.62698599847992	22.012467196193853	19.119312086113293
8	26.562725897634987	34.74407298911733	19.365571432467664	19.327629680780017
9	24.63192035707726	14.515857697191514	21.861086365032957	38.99113558069827
10	16.258885357259487	27.561713876230243	34.09083284317192	22.088567923338353
11	32.97608873156525	22.477688101905947	23.661726595888727	20.884496570640085
12	20.707024751200343	26.985829528095845	31.358326778404965	20.94881894229885
13	30.966696458504344	20.985143583704648	26.461427213262816	21.586732744528195
14	20.429123142465244	22.910204762365645	27.812341833943048	28.848330261226064
15	22.873421537447086	29.362667874752635	25.54136387081171	22.222546716988564
16	21.909141089975776	28.409393431587667	26.804447680810426	22.87701779762613
17	21.540560625585147	27.062461246676172	27.23674711750092	24.160231010237755
18	22.667396860344184	26.137377621559025	29.368919092110847	21.826306425985948
19	22.542300105256984	25.306351888507244	30.450138335337023	21.70120967089875
20	23.509259645881812	24.94938082778186	30.18580114419	21.35555838214633
21	22.324810840334976	25.88194660494937	30.694877115306962	21.09836543940869
22	21.321285298560795	26.07631360643832	30.21266448364824	22.389736611352646
23	22.103870131080065	26.16518226401715	30.986777589168547	20.74417001573424
24	22.157041682586353	26.360587112403405	28.859867256968236	22.622503948042006
25	22.116638061514394	26.53482471127944	29.78864368616186	21.55989354104431
26	21.89321134690081	27.0513104265237	29.792022722571698	21.263455504003794
27	21.04985213095311	27.058116771292095	29.512890179144442	22.379140918610357
28	21.323650624047687	26.697163274797965	29.81531393782525	22.163872163329103
29	21.732151990023155	26.62625178214001	29.301700403529356	22.339895824307483
30	21.738282527509583	26.823490964577317	30.458030798951434	20.980195708961663
31	22.32703134997573	26.570594225275045	30.24119320533704	20.861181219412178
32	21.846049653009164	26.39172251932266	29.52698558816835	22.235242239499826
33	21.49634352056494	26.46200647664736	30.06524195228173	21.976408050505974
34	21.013237857855042	26.717365085333945	30.61812471685485	21.651272339956165
35	20.73721885511971	28.315045907830022	29.526285644912026	21.421449592138238
36	22.394418990377712	26.532604201638687	29.451536532331463	21.621440275652134
37	21.57676458711916	26.84422376654913	29.594904220006164	21.98410742632554
38	21.443920184263682	25.84038445710832	30.591430329217108	22.124265029410893
39	20.96861044127078	25.58476035270382	31.248894270674814	22.197734935350585
40	20.164906631190004	26.08386816641175	30.94405691455841	22.807168287839836
41	21.386259341527175	25.122001316376043	30.93642994666191	22.555309395434875
42	20.0167117486441	26.922617411257455	30.446735162952827	22.613935677145623
43	20.496776278585138	26.64346073185584	29.814300226902297	23.045462762656722
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1174.0
1	1153.5
2	1133.0
3	2153.5
4	3174.0
5	3174.0
6	4975.0
7	6776.0
8	7306.0
9	7836.0
10	11935.0
11	16034.0
12	16034.0
13	29886.5
14	43739.0
15	65185.0
16	86631.0
17	80946.0
18	75261.0
19	75261.0
20	82798.0
21	90335.0
22	75031.0
23	59727.0
24	67206.0
25	74685.0
26	74685.0
27	83887.5
28	93090.0
29	102235.0
30	111380.0
31	123973.5
32	136567.0
33	136567.0
34	152101.5
35	167636.0
36	181762.0
37	195888.0
38	206901.0
39	217914.0
40	217914.0
41	224022.0
42	230130.0
43	241467.5
44	252805.0
45	265563.0
46	278321.0
47	278321.0
48	344405.5
49	410490.0
50	384911.5
51	359333.0
52	328203.5
53	297074.0
54	297074.0
55	270317.5
56	243561.0
57	210718.5
58	177876.0
59	159943.0
60	142010.0
61	142010.0
62	126149.0
63	110288.0
64	96449.5
65	82611.0
66	70457.0
67	58303.0
68	58303.0
69	49562.0
70	40821.0
71	34487.0
72	28153.0
73	23122.5
74	18092.0
75	18092.0
76	14609.5
77	11127.0
78	8626.5
79	6126.0
80	4792.0
81	3458.0
82	3458.0
83	2623.5
84	1789.0
85	1353.5
86	918.0
87	689.0
88	460.0
89	460.0
90	377.0
91	294.0
92	198.0
93	102.0
94	75.0
95	48.0
96	48.0
97	32.5
98	17.0
99	11.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4143193.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.797445950896666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23791454258944	37.69012960251983
2	12.733339268916627	12.427088694987782
3	4.297365774105773	6.291014222794791
4	1.9058446626657357	3.720014076689446
5	0.9793512694172865	2.3894920318166037
6	0.6159091886185302	1.8032877205364006
7	0.37855785366906175	1.2930859482592445
8	0.2728554136725918	1.065171784087828
9	0.2054704902579403	0.9023791628579476
>10	1.1710376980588753	10.830459931959826
>50	0.11791821421904061	4.02373191366308
>100	0.0732547944886261	7.0042084782319325
>500	0.006728286710564042	2.2463825471597807
>1k	0.003957815653364992	4.009188420392266
>5k	2.4736347833531185E-4	1.004186170581478
>10k+	2.4736347833531185E-4	3.3001792934616123
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39606	0.9559294003441308	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39359	0.9499678146781962	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29280	0.7067013291439719	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16837	0.4063774002321398	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11620	0.2804600220168358	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8924	0.21538943515303297	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8831	0.213144789537924	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8594	0.20742456361554965	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7864	0.18980530233566237	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7383	0.1781958986704216	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4988	0.12039024008777771	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4981	0.12022128826728565	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4872	0.11759046706248057	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4735	0.11428383857570719	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4622	0.11155647347347807	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4611	0.11129097775556196	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4496	0.10851534070462081	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4328	0.10446049701281113	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4147	0.10009188565437332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.4135974356009967E-5	0.0	0.0	4.827194871201993E-5	0.0
3	2.4135974356009967E-5	0.0	0.0	7.24079230680299E-5	0.0
4	2.4135974356009967E-5	0.0	0.0	7.24079230680299E-5	0.0
5	4.827194871201993E-5	0.0	0.0	7.24079230680299E-5	0.0
6	9.654389742403987E-5	0.0	0.0	7.24079230680299E-5	0.0
7	9.654389742403987E-5	0.0	0.0	7.24079230680299E-5	0.0
8	1.2067987178004983E-4	0.0	0.0	7.24079230680299E-5	0.0
9	1.2067987178004983E-4	0.0	0.0	1.6895182049206977E-4	0.0
10	1.2067987178004983E-4	0.0	0.0	2.896316922721196E-4	0.0
11	1.2067987178004983E-4	0.0	0.0	4.585835127641894E-4	0.0
12	1.2067987178004983E-4	0.0	0.0	7.723511793923189E-4	0.0
13	1.2067987178004983E-4	0.0	0.0	8.930310511723687E-4	0.0
14	1.448158461360598E-4	0.0	0.0	0.0010378468973084286	0.0
15	1.448158461360598E-4	0.0	0.0	0.0012067987178004983	0.0
16	1.448158461360598E-4	0.0	0.0	0.0016412462562086776	0.0
17	1.448158461360598E-4	0.0	0.0	0.0026066852304490766	0.0
18	1.448158461360598E-4	0.0	0.0	0.0033549004354853852	0.0
19	1.9308779484807973E-4	0.0	0.0	0.004272067461013764	0.0
20	1.9308779484807973E-4	0.0	0.0	0.0068546167171068305	0.0
21	1.9308779484807973E-4	0.0	0.0	0.011416315870392714	0.0
22	1.9308779484807973E-4	0.0	0.0	0.018150252715719496	0.0
23	1.9308779484807973E-4	0.0	0.0	0.022615407971581337	0.0
24	2.172237692040897E-4	0.0	0.0	0.031521582508949014	0.0
25	2.172237692040897E-4	0.0	0.0	0.038110703508139734	0.0
26	2.172237692040897E-4	0.0	0.0	0.05032350653228078	0.0
27	2.172237692040897E-4	0.0	0.0	0.10528112014091548	0.0
28	2.172237692040897E-4	0.0	0.0	0.2143033163070125	0.0
29	2.172237692040897E-4	2.4135974356009967E-5	0.0	0.34840278982900386	0.0
30	2.4135974356009966E-4	2.4135974356009967E-5	0.0	0.5346359679599768	0.0
31	2.4135974356009966E-4	2.4135974356009967E-5	0.0	0.7805574106733623	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13115	0.0	22.005337	1
GTATTGG	1435	0.0	19.59582	1
TGGACCC	2120	0.0	17.103773	5
ACCGATC	120	1.0430995E-7	16.958334	8
TTGGACC	2135	0.0	16.637003	4
ATTGGAC	1725	0.0	16.194204	3
GGACCCT	2200	0.0	16.145454	6
CTAATAC	1085	0.0	15.5161295	3
GTATCAA	19350	0.0	14.933849	2
GACCCTC	2200	0.0	14.799999	7
TAATACT	1150	0.0	14.639131	4
GTATTAG	1065	0.0	14.41784	1
CGAACTA	570	0.0	13.956141	24
TTAACGG	515	0.0	13.291262	35
TATACTG	655	0.0	13.274809	5
TCTAATA	1050	0.0	12.861904	2
TATTGGA	2180	0.0	12.644495	2
CCCTCGC	2515	0.0	12.50497	9
GGGTAAG	555	0.0	12.333334	1
CGTACAC	120	5.1264383E-4	12.333334	3
>>END_MODULE