FastQCFastQC Report
Wed 25 May 2016
SRR2088658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4002231
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT582841.4562877555043674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT506201.264794560833695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT398560.9958445676923696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213740.534052132423141No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG145800.364296813452297No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG134270.33548788163401866No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA120970.3022564164837062No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA119910.29960789369729035No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA109130.2726729166807213No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG102000.25485785303247116No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA67240.16800629448924861No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA65070.1625843185963029No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC64760.1618097506115964No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61050.1525399208591408No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60720.1517153807463887No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52380.13087700335138078No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52150.13030232387885657No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG49720.12423071032131829No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48040.12003305156548935No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC44290.11066327755694261No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42150.10531625985606527No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG42120.1052413016639969No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41910.10471659431951828No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA41350.10331737473424198No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT41240.10304252802999128No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC40560.10134347567644147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152250.024.6666661
GTATTGG13700.017.5547451
ACCGATC1352.2238964E-816.4444458
TTGGACC22050.016.1927434
TGGACCC21900.015.9657535
GTATCAA238100.015.7650162
GGACCCT21750.015.7356326
ATTGGAC16700.015.0658683
TATACCG2151.2732926E-1114.6279075
ATACTGG8150.014.5276076
CGCTAGA1458.925108E-714.0344831
ATTAGAG6650.013.6315783
GACCCTC23600.013.561447
TAATACT10550.013.502374
CGTTCGC3700.013.523
GTATTAG9200.013.4728261
CTAATAC10100.013.3712873
GGACCGT3600.013.3611116
ACGTTCG3900.013.28205222
ACCGTCG1409.572181E-613.21428623
Produced by FastQC (version 0.11.5)