FastQCFastQC Report
Wed 25 May 2016
SRR2088657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334365
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT590991.3634984594052415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT508881.174058945197278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT427850.9871111454619074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214740.49543589430054924No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG148100.34168788276944834No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG138210.31887023820098215No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA126020.2907461646631052No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA121410.28011023529398194No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG111090.25630051922253894No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107310.24757951856846389No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA76600.17672715611167955No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC71880.16583744100923664No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA66790.15409408298562766No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA64880.14968743979798654No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60340.13921301044097578No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG53480.12338600925395068No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53020.12232472346006855No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.11909472321781853No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.11591086583617209No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA46390.10702836516998454No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC44910.10361379348532024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157650.023.4931811
GTATTGG14950.020.9130421
ATTGGAC16650.019.03
TTGGACC22650.016.4988964
GGACCCT21950.016.1822326
TGGACCC24050.015.4615385
TATTGGA20050.015.1321692
CTCTAAT8350.015.0658681
GTATCAA251700.014.7588412
TCTAATA9550.014.7225122
CTAATAC10350.014.6570043
ATACTGG9950.014.5025136
GACCCTC24600.014.4390257
TTAACGG5150.014.36893335
TAATACT11000.014.2954554
TAGTACT7150.013.9720274
AATACTG11300.013.7522135
GTATTAG10000.013.6900011
ATCGTAC950.001246047813.63157925
GATACTG15750.013.62539736
Produced by FastQC (version 0.11.5)