FastQCFastQC Report
Wed 25 May 2016
SRR2088656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3349948
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT436241.3022291689303835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT383651.1452416574824444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT314950.9401638473194212No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164190.4901270109267368No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG108390.32355726118733785No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG99290.2963926604233857No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA94690.282661103993256No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA90580.2703922568350315No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG80640.2407201544620991No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA74810.22331689924739132No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA54610.16301745579334365No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC53390.15937560821839622No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49740.14847991670318464No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48810.145703754207528No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45650.13627077196422152No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA39340.11743465868723933No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38910.11615105667311851No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG38760.11570328852865777No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37330.11143456555146529No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA34660.10346429258006394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117700.024.5985571
GTATTGG9850.019.9086281
GGACCCT15350.016.9934846
ATTGGAC11300.016.5353983
TGGACCC16500.016.3696965
TTGGACC16950.015.9351034
GTATCAA185000.015.642
GACCCTC16850.014.931757
CATTGCG1752.242814E-914.829
TGTTACG1001.0943568E-414.816
AATACTG7850.014.3757965
GTATTAG5800.014.3534481
CGAACTA2850.014.28070224
TATTGGA13000.014.0884612
ACGCGAA800.0063019413.87500134
TAGGTCG2009.858923E-1013.87500121
ATACGAC950.001245944313.63157919
ACCCTCG19400.013.4458768
TATACCG1805.1682946E-813.3611115
TAATACT8900.013.303374
Produced by FastQC (version 0.11.5)