FastQCFastQC Report
Wed 25 May 2016
SRR2088655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4113070
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT526911.281062563972896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT459611.117437826246575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT383170.9315912444962036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206750.502665891900697No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG142480.34640791428300516No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG115270.2802529497431359No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA108500.26379322501197405No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA106210.25822560763614527No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA102410.24898676657581806No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG95000.23097102650818002No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC61810.15027704366811165No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA61530.14959628695840332No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA59150.1438098549258826No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58760.14286165808021745No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56940.13843673946711338No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG52950.1287359563537698No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51510.12523492184669846No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49300.1198618063879292No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45940.11169272587142937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153400.022.2024141
GTATTGG16700.019.275451
ATTGGAC17100.018.2836273
TTGGACC21550.017.4269144
GGACCCT23200.015.8685346
TGGACCC23700.015.61181455
TATTGGA20850.015.1726622
TAGGTCG2450.015.1020421
CTCGCGT1001.0944532E-414.79999921
CAATGCG2006.184564E-1114.79999919
GACCCTC24800.014.54637057
GTATCAA235700.014.4420872
ATATCGT1203.3044267E-513.87500126
ATACGAC1601.7867933E-713.87519
TTAACGG2202.8558134E-1013.45454535
CGACGAG2551.6370905E-1113.05882424
ATAAACG3150.012.9206353
TAAGCCG2451.1459633E-1012.83673410
ACCCTCG27600.012.8025368
TCTAACG1602.6996076E-612.718752
Produced by FastQC (version 0.11.5)