##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4113070
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.535210682045285	31.0	31.0	34.0	30.0	34.0
2	31.734399609051145	31.0	31.0	34.0	30.0	34.0
3	31.816176967569238	31.0	31.0	34.0	30.0	34.0
4	35.523888239198456	37.0	35.0	37.0	33.0	37.0
5	35.40741052303997	37.0	35.0	37.0	33.0	37.0
6	35.46132937197762	37.0	35.0	37.0	33.0	37.0
7	35.315133951039	37.0	35.0	37.0	32.0	37.0
8	35.3531814435446	37.0	35.0	37.0	32.0	37.0
9	36.93486373925073	39.0	37.0	39.0	33.0	39.0
10	36.76727237805338	39.0	37.0	39.0	32.0	39.0
11	36.84969134977037	39.0	37.0	39.0	32.0	39.0
12	36.713095327820824	39.0	35.0	39.0	32.0	39.0
13	36.756712382721425	39.0	37.0	39.0	32.0	39.0
14	37.726497725543204	40.0	37.0	41.0	32.0	41.0
15	37.73711242453933	40.0	37.0	41.0	32.0	41.0
16	37.62440342615127	39.0	36.0	41.0	32.0	41.0
17	37.671728173845814	39.0	37.0	41.0	32.0	41.0
18	37.63394933711315	39.0	37.0	41.0	32.0	41.0
19	37.686942600053	40.0	37.0	41.0	32.0	41.0
20	37.564117800086066	39.0	36.0	41.0	32.0	41.0
21	37.50985516901001	39.0	36.0	41.0	32.0	41.0
22	37.385103584427206	39.0	36.0	41.0	31.0	41.0
23	37.21488231418381	39.0	36.0	41.0	31.0	41.0
24	37.06035443111836	39.0	36.0	41.0	31.0	41.0
25	37.24001317750488	39.0	36.0	41.0	31.0	41.0
26	37.114725983267974	39.0	36.0	41.0	31.0	41.0
27	36.997203791814876	39.0	36.0	41.0	31.0	41.0
28	36.829689502002154	39.0	35.0	41.0	30.0	41.0
29	36.71586065882662	39.0	35.0	40.0	30.0	41.0
30	36.52175431004092	39.0	35.0	40.0	30.0	41.0
31	36.35544812026053	38.0	35.0	40.0	30.0	41.0
32	36.18643567943167	38.0	35.0	40.0	30.0	41.0
33	36.04180016386786	38.0	35.0	40.0	30.0	41.0
34	35.855455170955025	38.0	35.0	40.0	29.0	41.0
35	35.702361739527895	38.0	35.0	40.0	27.0	41.0
36	35.493282633166956	38.0	34.0	40.0	27.0	41.0
37	35.4136919624514	38.0	34.0	40.0	26.0	41.0
38	35.21572158995592	38.0	34.0	40.0	25.0	41.0
39	35.110839591837724	38.0	34.0	40.0	25.0	41.0
40	34.93532397941197	38.0	34.0	40.0	24.0	41.0
41	34.79173609979893	38.0	33.0	40.0	23.0	41.0
42	34.517823669424544	38.0	33.0	40.0	22.0	41.0
43	34.16523424108999	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	1.0
14	8.0
15	17.0
16	39.0
17	104.0
18	307.0
19	656.0
20	1569.0
21	3017.0
22	5848.0
23	10124.0
24	16536.0
25	25232.0
26	37368.0
27	52118.0
28	70645.0
29	91479.0
30	115922.0
31	142871.0
32	174130.0
33	212744.0
34	259829.0
35	317236.0
36	406293.0
37	562754.0
38	744435.0
39	861783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55900580345095	21.408923261699897	14.079288706489312	22.95278222835984
2	18.715266212342605	23.934530654717765	34.790460653477815	22.559742479461814
3	20.12900339648973	24.65727546577131	32.6742554831306	22.53946565460836
4	13.755175574449256	16.38578968993963	36.163571249699125	33.69546348591198
5	12.608611086123018	38.822290892204606	34.802422521376975	13.766675500295399
6	32.24148871767317	37.17850656565534	13.558631387260611	17.021373329410878
7	25.75448509264369	33.244924107783234	21.648087681464208	19.352503118108856
8	27.173619704989214	34.42083407284583	19.220679443821766	19.184866778343185
9	24.480935165217222	14.528101880104154	22.050779587996313	38.940183366682305
10	16.076434390856463	27.92354615895183	33.84277437534494	22.157245074846767
11	32.50545213186257	22.516927744969088	23.90717882263127	21.07044130053707
12	20.71192564191711	27.322462297019012	31.58839990566657	20.377212155397306
13	31.683292528451982	21.024903539205507	26.590527270384406	20.701276661958097
14	20.143907105884413	23.26267240771492	28.3715570121588	28.22186347424187
15	22.265898708264142	29.718458475056348	26.075875197844915	21.939767618834594
16	21.254610303252804	28.687598314640887	27.13736941019725	22.92042197190906
17	21.135964133846493	27.013957943822987	27.562696477327155	24.287381445003366
18	22.736714911246345	25.80228393876107	30.074664423411225	21.386336726581362
19	22.203001650835024	25.045258164825785	31.321494649981645	21.430245534357546
20	23.443778005236965	24.692115621664595	31.029766087131993	20.834340285966444
21	22.084574296085403	25.666302785996837	31.62508782977192	20.62403508814584
22	20.712338958490857	25.870359609731903	31.270681024149845	22.146620407627392
23	21.701721585093374	26.142808170053026	32.020996481946575	20.134473762907028
24	21.703423476867645	26.117377044397493	29.749992098359616	22.429207380375242
25	21.54872151458643	26.598331659806423	30.681826470252147	21.171120355355004
26	21.415123010306168	27.100851675269322	30.66595025127216	20.818075063152342
27	20.27222974566443	27.177898747164527	30.57640643120589	21.973465075965155
28	20.837500942118663	26.670807936650725	30.561332532633774	21.93035858859684
29	20.944744436637354	26.602124447189084	30.113418930385333	22.339712185788233
30	21.17270068343111	26.885124736510686	31.54954814773393	20.392626432324274
31	21.869236361160883	26.642726722375258	31.2256052048713	20.26243171159256
32	21.515267184852192	26.53560479155473	30.224746965162275	21.724381058430808
33	21.067864150136028	26.434366543725247	30.90465272898346	21.593116577155264
34	20.23746252799004	27.1042797715575	31.430537287233136	21.227720413219323
35	19.851984041117703	28.9711334842344	30.318642765622762	20.85823970902513
36	21.902423250759163	26.63120248378948	30.355987133698186	21.110387131753168
37	21.139076164519448	27.33145801068302	30.244561847962714	21.28490397683482
38	20.885372726454936	25.96996890400601	31.491999893023948	21.65265847651511
39	20.423041669604455	25.737125796546135	32.22318122472995	21.616651309119465
40	19.378347560338142	26.23184142258702	31.87570354990311	22.514107467171723
41	20.80810683990304	25.37301820781071	31.733133644698487	22.085741307587764
42	19.45186928498664	27.267855883804554	31.19531153128928	22.084963299919526
43	19.814980051397132	27.28660100606117	30.37169316350074	22.52672577904096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1521.0
1	1339.0
2	1157.0
3	2355.5
4	3554.0
5	3554.0
6	5711.0
7	7868.0
8	8565.0
9	9262.0
10	14359.5
11	19457.0
12	19457.0
13	36377.0
14	53297.0
15	81526.0
16	109755.0
17	101826.0
18	93897.0
19	93897.0
20	99626.0
21	105355.0
22	86539.5
23	67724.0
24	74820.5
25	81917.0
26	81917.0
27	90385.5
28	98854.0
29	106232.0
30	113610.0
31	122904.5
32	132199.0
33	132199.0
34	143896.0
35	155593.0
36	166849.5
37	178106.0
38	187204.0
39	196302.0
40	196302.0
41	202995.0
42	209688.0
43	218826.0
44	227964.0
45	249136.0
46	270308.0
47	270308.0
48	353403.5
49	436499.0
50	402675.0
51	368851.0
52	336422.0
53	303993.0
54	303993.0
55	270367.5
56	236742.0
57	202944.5
58	169147.0
59	151180.5
60	133214.0
61	133214.0
62	116289.5
63	99365.0
64	86459.5
65	73554.0
66	63058.5
67	52563.0
68	52563.0
69	44982.0
70	37401.0
71	31490.0
72	25579.0
73	20894.5
74	16210.0
75	16210.0
76	13189.0
77	10168.0
78	7956.5
79	5745.0
80	4554.5
81	3364.0
82	3364.0
83	2510.0
84	1656.0
85	1259.0
86	862.0
87	625.5
88	389.0
89	389.0
90	310.0
91	231.0
92	158.5
93	86.0
94	63.5
95	41.0
96	41.0
97	30.0
98	19.0
99	11.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4113070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.48761929552579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6852772770211	36.41601252903013
2	12.867814550393172	12.221237570689967
3	4.361249614068882	6.213160839769855
4	2.027470926484341	3.8511906995854135
5	1.0637983729860296	2.525862607178016
6	0.6108272328147949	1.7404038652349088
7	0.4041842405575711	1.3435623138594404
8	0.2923101454647219	1.1104890323238783
9	0.21116138337817325	0.9024796245401334
>10	1.279682298813136	11.465078556767146
>50	0.11614664578417741	3.8279231080846765
>100	0.07000986977632162	6.238200703097941
>500	0.005239179270487108	1.692935840944897
>1k	0.003955066653197142	3.990177977095425
>5k	4.1091601591658624E-4	1.2138568897904713
>10k+	4.622805179061595E-4	5.247427842007714
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	52691	1.281062563972896	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45961	1.117437826246575	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38317	0.9315912444962036	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20675	0.502665891900697	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14248	0.34640791428300516	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11527	0.2802529497431359	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10850	0.26379322501197405	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10621	0.25822560763614527	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10241	0.24898676657581806	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	9500	0.23097102650818002	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6181	0.15027704366811165	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6153	0.14959628695840332	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5915	0.1438098549258826	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5876	0.14286165808021745	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5694	0.13843673946711338	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5295	0.1287359563537698	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5151	0.12523492184669846	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4930	0.1198618063879292	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4594	0.11169272587142937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.431273963244E-5	0.0
5	2.431273963244E-5	0.0	0.0	2.431273963244E-5	0.0
6	7.293821889732E-5	0.0	0.0	2.431273963244E-5	0.0
7	7.293821889732E-5	0.0	0.0	2.431273963244E-5	0.0
8	1.4587643779464E-4	0.0	0.0	2.431273963244E-5	0.0
9	1.4587643779464E-4	0.0	0.0	1.7018917742708E-4	0.0
10	1.9450191705952E-4	0.0	2.431273963244E-5	2.1881465669196002E-4	0.0
11	2.1881465669196002E-4	0.0	2.431273963244E-5	5.3488027191368E-4	0.0
12	2.4312739632440002E-4	0.0	2.431273963244E-5	0.0010211350645624802	0.0
13	2.4312739632440002E-4	0.0	2.431273963244E-5	0.0013372006797842	0.0
14	2.6744013595684E-4	0.0	2.431273963244E-5	0.00153170259684372	0.0
15	2.9175287558928E-4	0.0	2.431273963244E-5	0.00213952108765472	0.0
16	3.1606561522172E-4	0.0	2.431273963244E-5	0.0029418414955252404	0.0
17	3.1606561522172E-4	0.0	2.431273963244E-5	0.00396297656008772	0.0
18	3.1606561522172E-4	0.0	2.431273963244E-5	0.004789609707590681	0.0
19	3.646910944866E-4	0.0	2.431273963244E-5	0.00622406134590464	0.0
20	3.8900383411904E-4	0.0	2.431273963244E-5	0.010600354479743841	0.0
21	4.3762931338392003E-4	0.0	2.431273963244E-5	0.018647871298081483	0.0
22	4.3762931338392003E-4	0.0	2.431273963244E-5	0.029637229611944364	0.0
23	4.3762931338392003E-4	0.0	2.431273963244E-5	0.036469109448660005	0.0
24	4.3762931338392003E-4	0.0	2.431273963244E-5	0.047847471596641926	0.0
25	4.3762931338392003E-4	0.0	2.431273963244E-5	0.05652711964542301	0.0
26	4.3762931338392003E-4	0.0	2.431273963244E-5	0.0700206901414272	0.0
27	4.3762931338392003E-4	0.0	2.431273963244E-5	0.12739875567398562	0.0
28	4.3762931338392003E-4	0.0	2.431273963244E-5	0.24728487480154726	0.0
29	4.3762931338392003E-4	0.0	2.431273963244E-5	0.3980238605226753	0.0
30	4.3762931338392003E-4	0.0	2.431273963244E-5	0.6261989219731247	0.0
31	4.3762931338392003E-4	0.0	2.431273963244E-5	0.8959001427157817	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15340	0.0	22.202414	1
GTATTGG	1670	0.0	19.27545	1
ATTGGAC	1710	0.0	18.283627	3
TTGGACC	2155	0.0	17.426914	4
GGACCCT	2320	0.0	15.868534	6
TGGACCC	2370	0.0	15.6118145	5
TATTGGA	2085	0.0	15.172662	2
TAGGTCG	245	0.0	15.10204	21
CTCGCGT	100	1.0944532E-4	14.799999	21
CAATGCG	200	6.184564E-11	14.799999	19
GACCCTC	2480	0.0	14.5463705	7
GTATCAA	23570	0.0	14.442087	2
ATATCGT	120	3.3044267E-5	13.875001	26
ATACGAC	160	1.7867933E-7	13.875	19
TTAACGG	220	2.8558134E-10	13.454545	35
CGACGAG	255	1.6370905E-11	13.058824	24
ATAAACG	315	0.0	12.920635	3
TAAGCCG	245	1.1459633E-10	12.836734	10
ACCCTCG	2760	0.0	12.802536	8
TCTAACG	160	2.6996076E-6	12.71875	2
>>END_MODULE