FastQCFastQC Report
Wed 25 May 2016
SRR2088654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4916087
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT605471.231609611465379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT532081.0823242143599168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT436810.8885318750461495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231740.47139116944024795No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG151650.3084770468870872No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA127970.2603086560510422No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG127900.26016626638218565No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA115720.2353904640011456No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA105790.21519147240478048No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG103860.21126558582059266No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA70740.14389493107017837No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA69310.14098611354925167No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC68510.1393588030480339No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT65090.13240205065532812No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA63430.1290253813653013No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55670.11324046950348926No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55120.11212169353390206No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG55050.11197930386504552No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53380.1085822931937535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166550.023.015311
GTATTGG16450.019.9057751
ATTGGAC18350.018.0463223
TTGGACC25300.016.745064
TGGACCC25300.015.7944675
GGACCCT26550.015.19020756
ACCGTTA2750.014.88
GTATCAA259050.014.7971442
TATTGGA22150.014.5327312
GTATTAG10500.014.2714291
ACGTTTA2252.7284841E-1113.977777526
GACCCTC28700.013.9233457
TCTATAC4200.013.2142873
TAATACT12100.012.9958694
ACGTTCG4300.012.90697722
CTAGACT3600.012.8472224
GGACCGT4850.012.5876296
TTAACGG5300.012.56603835
TATACTG6900.012.3333345
ATTAGAG8100.012.3333333
Produced by FastQC (version 0.11.5)