##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4916087
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.561631028905712	31.0	31.0	34.0	30.0	34.0
2	31.764026552011792	31.0	31.0	34.0	30.0	34.0
3	31.840438543906973	31.0	31.0	34.0	30.0	34.0
4	35.55033383257864	37.0	35.0	37.0	33.0	37.0
5	35.43811551748372	37.0	35.0	37.0	33.0	37.0
6	35.487249513688425	37.0	35.0	37.0	33.0	37.0
7	35.34106332943254	37.0	35.0	37.0	32.0	37.0
8	35.37374745402187	37.0	35.0	37.0	33.0	37.0
9	36.96463894963616	39.0	37.0	39.0	33.0	39.0
10	36.78584105610824	39.0	37.0	39.0	32.0	39.0
11	36.87383298953009	39.0	37.0	39.0	33.0	39.0
12	36.7323816685913	39.0	35.0	39.0	32.0	39.0
13	36.76529402347843	39.0	37.0	39.0	32.0	39.0
14	37.743778944514204	40.0	37.0	41.0	32.0	41.0
15	37.760187116297985	40.0	37.0	41.0	32.0	41.0
16	37.65199252983114	39.0	36.0	41.0	32.0	41.0
17	37.68354567362213	39.0	37.0	41.0	32.0	41.0
18	37.6463020284222	39.0	37.0	41.0	32.0	41.0
19	37.694173028264146	40.0	37.0	41.0	32.0	41.0
20	37.57467554988347	39.0	37.0	41.0	32.0	41.0
21	37.509888453967555	39.0	36.0	41.0	32.0	41.0
22	37.37966516865954	39.0	36.0	41.0	31.0	41.0
23	37.198127291075195	39.0	36.0	41.0	31.0	41.0
24	37.038827628559055	39.0	36.0	41.0	31.0	41.0
25	37.22276965399514	39.0	36.0	41.0	31.0	41.0
26	37.09870533210661	39.0	36.0	41.0	31.0	41.0
27	36.99042612549371	39.0	36.0	41.0	31.0	41.0
28	36.81965270346111	39.0	35.0	41.0	30.0	41.0
29	36.7025662890018	39.0	35.0	41.0	30.0	41.0
30	36.49747736360239	39.0	35.0	40.0	30.0	41.0
31	36.340413829128735	38.0	35.0	40.0	30.0	41.0
32	36.16492405443598	38.0	35.0	40.0	30.0	41.0
33	36.021277288217235	38.0	35.0	40.0	29.0	41.0
34	35.83362621532125	38.0	35.0	40.0	28.0	41.0
35	35.69232562401764	38.0	35.0	40.0	27.0	41.0
36	35.49301243041468	38.0	34.0	40.0	27.0	41.0
37	35.41496356756908	38.0	34.0	40.0	26.0	41.0
38	35.22623033318979	38.0	34.0	40.0	25.0	41.0
39	35.1257117703572	38.0	34.0	40.0	25.0	41.0
40	34.95141461084802	38.0	34.0	40.0	24.0	41.0
41	34.81332897485338	38.0	33.0	40.0	23.0	41.0
42	34.53883139171459	38.0	33.0	40.0	22.0	41.0
43	34.18384682777176	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	5.0
13	8.0
14	13.0
15	30.0
16	73.0
17	147.0
18	375.0
19	869.0
20	1959.0
21	3902.0
22	7059.0
23	12121.0
24	20018.0
25	30674.0
26	44170.0
27	61740.0
28	83929.0
29	109259.0
30	137583.0
31	167671.0
32	206626.0
33	252726.0
34	309681.0
35	379500.0
36	487352.0
37	674281.0
38	898871.0
39	1025443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.410577152113056	21.348686465475488	14.000789652420718	23.23994672999074
2	18.904588140934038	23.503042155275118	34.85164115281117	22.74072855097967
3	19.96183143219394	24.362933365499835	32.59948410188835	23.075751100417875
4	13.777624358559967	16.31346231260757	36.08215232968823	33.82676099914424
5	12.718997853374034	38.62264846004556	34.789620281333505	13.868733405246896
6	32.549464645357176	37.0754830010128	13.57673287718464	16.798319476445393
7	26.161843759070987	33.11056130617705	21.38052072715556	19.347074207596407
8	27.155988085646165	34.310458704249946	18.990205014679358	19.54334819542453
9	24.59543535336132	14.458674144700856	21.810679916771207	39.13521058516662
10	16.263544562982712	27.84832733838925	33.633700949556015	22.254427149072015
11	33.12935674246611	22.40354981512736	23.56886686504938	20.898226577357153
12	21.06150684477309	27.18405919179217	31.022905005546075	20.731528957888663
13	31.586625704549164	20.922921014213134	26.317760446468906	21.172692834768792
14	20.4203465072933	22.98761189539567	27.9883777484003	28.603663848910728
15	22.681555472879143	29.565038210267637	25.63243897026232	22.1209673465909
16	21.685987249615398	28.41170223391083	26.824749033123297	23.07756148335048
17	21.586639943516055	26.896777864183445	27.241930421491727	24.274651770808774
18	22.904415646020908	25.70361346330933	29.664487223273305	21.727483667396445
19	22.58645951546423	24.906475414287826	30.95557503355819	21.55149003668975
20	23.622100259820463	24.584491690240633	30.57297399334064	21.22043405659827
21	22.316264948118288	25.50917019979508	31.224447411121893	20.950117440964735
22	21.165085158175597	25.787989512797477	30.781717247884345	22.265208081142585
23	22.180526910935466	25.965325674667678	31.362443341625156	20.4917040727717
24	22.01354451212926	25.963983143504173	29.30249200227742	22.719980342089148
25	21.9538628994971	26.327910795720257	30.28996435579761	21.428261948985035
26	21.70061270274509	26.905626365033818	30.22745529116958	21.166305641051512
27	20.664585472144818	26.963823056833615	30.07159962791545	22.299991843106113
28	21.281173421056216	26.478396334320365	30.02802838924535	22.212401855378065
29	21.376208354327332	26.468469740262933	29.64003688299251	22.51528502241722
30	21.55378861277272	26.703473718020042	31.029088785450703	20.713648883756534
31	22.262177215334063	26.492350521868307	30.596264874889318	20.649207387908312
32	21.785944797152695	26.38612782890132	29.794651721989464	22.033275651956526
33	21.432045445900368	26.30848477661197	30.399990887061197	21.85947889042647
34	20.732403637283067	26.788215912370955	31.011615538943882	21.467764911402096
35	20.37602263751638	28.611841084179346	29.86519563221725	21.14694064608702
36	22.30161915360733	26.45600047354736	29.826831787151043	21.41554858569427
37	21.42020676200401	27.0757209951736	29.885760768676388	21.618311474146
38	21.317198007276925	25.787826781747352	30.972641452439714	21.922333758536006
39	20.820054649154905	25.544686251484155	31.744291750735897	21.890967348625033
40	19.769117186087225	26.127568531639085	31.39590491380645	22.70740936846724
41	21.122388598899896	25.192861721120885	31.34791959540179	22.33683008457743
42	19.810125410717912	27.017768399948984	30.867151049198277	22.304955140134826
43	20.19522844083109	26.85196580125616	30.13622419619506	22.816581561717683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1548.0
1	1501.0
2	1454.0
3	2607.5
4	3761.0
5	3761.0
6	6131.5
7	8502.0
8	9393.5
9	10285.0
10	15754.0
11	21223.0
12	21223.0
13	39940.5
14	58658.0
15	91060.0
16	123462.0
17	113933.5
18	104405.0
19	104405.0
20	111048.5
21	117692.0
22	95616.0
23	73540.0
24	82183.5
25	90827.0
26	90827.0
27	100031.0
28	109235.0
29	117943.0
30	126651.0
31	139461.0
32	152271.0
33	152271.0
34	166702.0
35	181133.0
36	197941.0
37	214749.0
38	227487.5
39	240226.0
40	240226.0
41	248100.0
42	255974.0
43	270556.0
44	285138.0
45	305418.0
46	325698.0
47	325698.0
48	417557.0
49	509416.0
50	475737.5
51	442059.0
52	404164.5
53	366270.0
54	366270.0
55	329273.0
56	292276.0
57	250577.0
58	208878.0
59	186352.0
60	163826.0
61	163826.0
62	145134.0
63	126442.0
64	110531.0
65	94620.0
66	82061.5
67	69503.0
68	69503.0
69	59484.0
70	49465.0
71	41998.5
72	34532.0
73	28569.0
74	22606.0
75	22606.0
76	18031.0
77	13456.0
78	10480.5
79	7505.0
80	5916.5
81	4328.0
82	4328.0
83	3267.5
84	2207.0
85	1667.5
86	1128.0
87	854.0
88	580.0
89	580.0
90	459.0
91	338.0
92	229.5
93	121.0
94	91.5
95	62.0
96	62.0
97	44.0
98	26.0
99	18.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4916087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.22830032171379
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85164889318204	32.3570955792995
2	13.122447510072256	11.345222038426575
3	4.757972805648839	6.170372320954056
4	2.3019560435374142	3.9803858870967765
5	1.226504385130815	2.6509849953166893
6	0.7652908687257487	1.9849334104045147
7	0.5043188275940823	1.5260592015992114
8	0.3717779098693059	1.285706171264752
9	0.26757857603630664	1.0410270340098593
>10	1.5765924928131971	12.956675535772682
>50	0.14783068352369833	4.455368754820858
>100	0.09295884975380368	7.691411577210309
>500	0.007788329307516921	2.2968460372070822
>1k	0.004436987005961625	3.9420905301558546
>5k	4.248179048260999E-4	1.1351088852386526
>10k+	4.720198942512221E-4	5.180712041222518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	60547	1.231609611465379	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	53208	1.0823242143599168	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	43681	0.8885318750461495	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23174	0.47139116944024795	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15165	0.3084770468870872	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12797	0.2603086560510422	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12790	0.26016626638218565	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11572	0.2353904640011456	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10579	0.21519147240478048	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10386	0.21126558582059266	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7074	0.14389493107017837	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6931	0.14098611354925167	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6851	0.1393588030480339	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6509	0.13240205065532812	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6343	0.1290253813653013	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5567	0.11324046950348926	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5512	0.11212169353390206	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5505	0.11197930386504552	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5338	0.1085822931937535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	2.034138126522171E-5
4	0.0	0.0	0.0	0.0	2.034138126522171E-5
5	0.0	0.0	0.0	2.034138126522171E-5	2.034138126522171E-5
6	0.0	0.0	0.0	2.034138126522171E-5	4.068276253044342E-5
7	0.0	0.0	0.0	2.034138126522171E-5	4.068276253044342E-5
8	0.0	0.0	0.0	2.034138126522171E-5	4.068276253044342E-5
9	0.0	0.0	0.0	1.8307243138699538E-4	4.068276253044342E-5
10	0.0	0.0	0.0	3.2546210024354734E-4	4.068276253044342E-5
11	2.034138126522171E-5	0.0	0.0	5.492172941609862E-4	4.068276253044342E-5
12	2.034138126522171E-5	0.0	0.0	6.916069630175381E-4	4.068276253044342E-5
13	2.034138126522171E-5	0.0	0.0	7.933138693436467E-4	4.068276253044342E-5
14	6.102414379566513E-5	0.0	0.0	9.967276819958638E-4	4.068276253044342E-5
15	6.102414379566513E-5	0.0	0.0	0.001403555307300298	4.068276253044342E-5
16	6.102414379566513E-5	0.0	0.0	0.002034138126522171	4.068276253044342E-5
17	6.102414379566513E-5	0.0	0.0	0.0028274519958658177	4.068276253044342E-5
18	6.102414379566513E-5	0.0	0.0	0.0033156451462311386	4.068276253044342E-5
19	6.102414379566513E-5	0.0	0.0	0.004108959015574785	4.068276253044342E-5
20	6.102414379566513E-5	0.0	0.0	0.00701777653650149	4.068276253044342E-5
21	6.102414379566513E-5	0.0	0.0	0.01224551152166347	4.068276253044342E-5
22	6.102414379566513E-5	0.0	0.0	0.020137967452569492	4.068276253044342E-5
23	6.102414379566513E-5	0.0	0.0	0.024450340280796495	4.068276253044342E-5
24	6.102414379566513E-5	0.0	0.0	0.031590165104889316	4.068276253044342E-5
25	6.102414379566513E-5	0.0	0.0	0.03708233804649918	4.068276253044342E-5
26	6.102414379566513E-5	0.0	0.0	0.046805518291275154	4.068276253044342E-5
27	6.102414379566513E-5	0.0	0.0	0.09957106129326027	4.068276253044342E-5
28	6.102414379566513E-5	0.0	0.0	0.21126558582059268	4.068276253044342E-5
29	6.102414379566513E-5	0.0	0.0	0.3589440138061023	4.068276253044342E-5
30	6.102414379566513E-5	0.0	0.0	0.5677279511123379	4.068276253044342E-5
31	6.102414379566513E-5	0.0	0.0	0.8219952169276094	4.068276253044342E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16655	0.0	23.01531	1
GTATTGG	1645	0.0	19.905775	1
ATTGGAC	1835	0.0	18.046322	3
TTGGACC	2530	0.0	16.74506	4
TGGACCC	2530	0.0	15.794467	5
GGACCCT	2655	0.0	15.1902075	6
ACCGTTA	275	0.0	14.8	8
GTATCAA	25905	0.0	14.797144	2
TATTGGA	2215	0.0	14.532731	2
GTATTAG	1050	0.0	14.271429	1
ACGTTTA	225	2.7284841E-11	13.9777775	26
GACCCTC	2870	0.0	13.923345	7
TCTATAC	420	0.0	13.214287	3
TAATACT	1210	0.0	12.995869	4
ACGTTCG	430	0.0	12.906977	22
CTAGACT	360	0.0	12.847222	4
GGACCGT	485	0.0	12.587629	6
TTAACGG	530	0.0	12.566038	35
TATACTG	690	0.0	12.333334	5
ATTAGAG	810	0.0	12.333333	3
>>END_MODULE