FastQCFastQC Report
Wed 25 May 2016
SRR2088653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4480468
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT514181.147603330723487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT452741.0104747986147875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT359410.8021706661000592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202320.4515599709673186No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG127320.28416674329556646No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG104670.2336139885386973No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102850.22955191288052942No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA94040.2098887884033543No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93520.2087281953581635No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG83320.1859627163948052No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA56210.1254556443657225No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT54830.12237560897656227No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC54720.12213009890931037No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA53770.12000978469213484No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA52670.1175546840196158No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49140.10967604277053201No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48130.10742181397121908No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG47850.10679687925457787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151050.021.7272421
TCTATAC4000.016.653
GTATTGG16050.015.9065431
GTATCAA227750.014.4182212
CTAATAC9550.014.3350783
TATACTG5800.014.0344845
ATTGGAC18550.013.9622643
TTGGACC24550.013.3380854
AATACTG11250.013.1555565
TAATACT11000.013.1181814
GTATACT3950.013.1139244
TATAGCG850.00941029113.0588245
ACCGTCC1703.7383506E-713.0588248
TGCGACG1857.398921E-813.022
CGAACTA6700.012.97761224
TGGACCC24300.012.9423885
CGCGATA1153.5807723E-412.86956514
GGACCCT23300.012.7832616
TAGGTCG1807.4764284E-712.33333421
CGCACTA2401.1314114E-912.33333429
Produced by FastQC (version 0.11.5)