FastQCFastQC Report
Wed 25 May 2016
SRR2088652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821535
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146201.7795955132769756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133481.6247634002203193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75930.9242454673264073No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.6507330789315123No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG30290.36870005538412853No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG27780.3381474921944896No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA27090.3297485804013219No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22270.2710779212084695No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18870.22969197903923755No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.2053473071749834No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16090.19585288514792432No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.17832472140566136No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC11830.14399873407706307No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC11290.13742567267371444No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA11100.13511292884661033No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.13024399447375948No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA10210.12427954986701722No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT9770.11892372205688133No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG9700.11807165854163244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG250.00549544229.64
GGTATCA38100.024.8123381
TATACCG601.3366316E-624.6666685
TTATGCG1300.024.1923084
ATGCGGA1201.0913936E-1121.5833346
GTAATAC957.1377144E-921.4210533
CGACCGC705.0989984E-621.14285910
TATGCGG1500.020.9666655
TGCGGAG1303.274181E-1119.9230777
TAATACC951.6746526E-719.4736844
GGGTAAG1254.110916E-1019.241
AGTGTTC1254.110916E-1019.248
GCTTATG1457.2759576E-1219.137932
CTATCCT701.21866E-418.54
CACGAGC500.007033215418.533
TGCGATT500.007033215418.529
ACGTATA500.007033215418.529
CTTATGC1700.018.4999983
GTATCAA52600.017.9020922
GGCTTAT2250.017.2666661
Produced by FastQC (version 0.11.5)