FastQCFastQC Report
Wed 25 May 2016
SRR2088651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637050
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82601.2966015226434346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73721.1572090102817674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61840.9707244329330508No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.5235067891060357No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG17700.2778431834235931No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15980.250843732831018No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG14830.23279177458598227No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13410.2105015304921121No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA12500.19621693744604035No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG11660.18303115924966645No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.14190408916097638No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8330.13075896711404128No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.1299740993642571No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.12950317871438663No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7750.12165450121654502No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC7530.1182010831174947No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA7140.11207911466917825No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG6990.10972451141982575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG250.00549452729.65
AGTCGTG358.865036E-426.42857220
GGTATCA20400.026.026961
TCTATAC400.001930247923.1253
CCGTTTA652.6797297E-622.7692327
CGCCGTT603.722931E-521.58333225
GCTAGTG450.003823964420.5555552
TAGGACA450.003823964420.5555554
GTATTGG2450.018.8775521
GACCGGC500.007031523618.57
GCCCGTG701.2181147E-418.524
CTAATAC1306.9485395E-1018.53
TTGGACC2800.017.8392874
CGTTTAC752.0654444E-417.26666628
GCCGTTT752.0654444E-417.26666626
TAATACT1452.9704097E-916.5862054
CGAGGCT904.4409535E-516.44444533
TTACGGC904.4409535E-516.44444518
GTATCAA32450.016.3620952
TATACTA1708.54925E-1116.323535
Produced by FastQC (version 0.11.5)