##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088651_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637050
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.599656227925596	31.0	31.0	34.0	30.0	34.0
2	31.796127462522566	31.0	31.0	34.0	30.0	34.0
3	31.880174240640454	31.0	31.0	34.0	30.0	34.0
4	35.575246840907305	37.0	35.0	37.0	33.0	37.0
5	35.47339769248882	37.0	35.0	37.0	33.0	37.0
6	35.51821678047249	37.0	35.0	37.0	33.0	37.0
7	35.37553567223923	37.0	35.0	37.0	33.0	37.0
8	35.416830704026374	37.0	35.0	37.0	33.0	37.0
9	37.00647672867122	39.0	37.0	39.0	33.0	39.0
10	36.83399105250765	39.0	37.0	39.0	32.0	39.0
11	36.92698061376658	39.0	37.0	39.0	33.0	39.0
12	36.78577034769641	39.0	37.0	39.0	32.0	39.0
13	36.82576877796092	39.0	37.0	39.0	32.0	39.0
14	37.790638097480574	40.0	37.0	41.0	32.0	41.0
15	37.80417706616435	40.0	37.0	41.0	32.0	41.0
16	37.69959971744761	39.0	37.0	41.0	32.0	41.0
17	37.73747743505219	39.0	37.0	41.0	32.0	41.0
18	37.71155168354132	39.0	37.0	41.0	32.0	41.0
19	37.767916176124324	40.0	37.0	41.0	32.0	41.0
20	37.635873165371635	39.0	37.0	41.0	32.0	41.0
21	37.5723757946786	39.0	37.0	41.0	32.0	41.0
22	37.43555136959422	39.0	36.0	41.0	32.0	41.0
23	37.24468566046621	39.0	36.0	41.0	31.0	41.0
24	37.08877011223609	39.0	36.0	41.0	31.0	41.0
25	37.28209245742092	39.0	36.0	41.0	31.0	41.0
26	37.16434502786281	39.0	36.0	41.0	31.0	41.0
27	37.04540930853151	39.0	36.0	41.0	31.0	41.0
28	36.87502864767287	39.0	35.0	41.0	30.0	41.0
29	36.760819401930775	39.0	35.0	41.0	30.0	41.0
30	36.54566831488894	39.0	35.0	40.0	30.0	41.0
31	36.38737618711247	38.0	35.0	40.0	30.0	41.0
32	36.19918687701122	38.0	35.0	40.0	30.0	41.0
33	36.04369201789498	38.0	35.0	40.0	30.0	41.0
34	35.841439447453105	38.0	35.0	40.0	28.0	41.0
35	35.70269994505926	38.0	35.0	40.0	27.0	41.0
36	35.508839180598066	38.0	34.0	40.0	27.0	41.0
37	35.41301938623342	38.0	34.0	40.0	26.0	41.0
38	35.2127399733145	38.0	34.0	40.0	25.0	41.0
39	35.10076289145279	38.0	34.0	40.0	24.0	41.0
40	34.9170944195903	38.0	34.0	40.0	24.0	41.0
41	34.76538262302802	38.0	33.0	40.0	23.0	41.0
42	34.48016953143395	38.0	33.0	40.0	22.0	41.0
43	34.12572796483793	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	3.0
16	8.0
17	15.0
18	51.0
19	81.0
20	204.0
21	454.0
22	885.0
23	1558.0
24	2486.0
25	3772.0
26	5610.0
27	7902.0
28	10632.0
29	13981.0
30	17630.0
31	21696.0
32	26414.0
33	32693.0
34	39860.0
35	48760.0
36	63295.0
37	88665.0
38	115725.0
39	134665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71132564162939	21.596421003060982	13.944117416215368	22.748135939094265
2	18.586610156188684	23.875049054234363	35.03586845616514	22.50247233341182
3	19.852601836590534	25.254218664155093	32.62930696177694	22.263872537477436
4	13.424534965858253	16.61094105643199	37.05533317635978	32.90919080134997
5	12.352719566753002	39.17651675692646	34.98124166078016	13.48952201554038
6	31.498783454987834	37.760144415665955	13.934228082568087	16.806844046778117
7	25.520131857781962	33.26881720430108	22.176281296601523	19.03476964131544
8	26.98516599952908	34.57169766894278	19.473196766344874	18.969939565183267
9	24.760850796640767	14.297778824268113	22.119770818617063	38.82159956047406
10	16.255082018679854	27.7748999293619	33.89608350992857	22.07393454202967
11	32.52994270465426	22.734793187347933	23.940350051016406	20.794914056981398
12	20.365905344949375	27.549642885173846	31.674593830939486	20.40985793893729
13	31.001805195824506	21.309944274389768	26.911388431049367	20.776862098736363
14	20.2745467388745	22.895063181853857	28.594929754336395	28.235460324935246
15	22.443293305078093	29.701436307982103	26.289145279020488	21.566125107919316
16	21.17981320147555	28.595871595636137	27.40538419276352	22.818931010124793
17	20.996939015775844	27.14151165528608	27.913978494623652	23.94757083431442
18	22.410956753786987	25.871909583235226	30.238599795934384	21.478533867043403
19	22.4192763519347	25.178557413075897	31.352013185778194	21.050153049211207
20	23.151087041833453	24.866023075111844	31.274782199199436	20.70810768385527
21	22.029981948041755	25.609449807707403	31.774272035162078	20.58629620908877
22	20.646417078722234	25.974884232006907	31.545404599325018	21.83329408994584
23	21.430499960756613	26.45851973942391	32.13468330586296	19.97629699395652
24	21.506318185385762	26.373283101797345	30.16403735970489	21.956361353112
25	21.225649478062948	26.62899301467703	31.078094341103522	21.0672631661565
26	21.2843575857468	27.08923946315046	30.95643983988698	20.66996311121576
27	20.38788164194333	27.232556314261046	30.642178792873402	21.73738325092222
28	20.622086178478927	26.850953614315987	30.838866650969308	21.688093556235774
29	21.09755906129817	26.847186249117023	30.298720665567853	21.75653402401695
30	20.86225570991288	26.999764539675063	31.623734400753474	20.514245349658584
31	21.396122753316067	26.824268110823326	31.559218271721218	20.220390864139393
32	21.01734557727023	26.682677968762263	30.67043403186563	21.629542422101874
33	20.81798916882505	26.78031551683541	31.167726238128875	21.23396907621066
34	20.182717212149754	27.262538262302805	31.635978337650105	20.91876618789734
35	19.79326583470685	28.99505533317636	30.47829840671847	20.73338042539832
36	21.543363943175574	27.025508201867986	30.583784632289458	20.847343222666982
37	20.87858095910839	27.6025429715093	30.308296052115217	21.21058001726709
38	20.73542108154776	26.483164586767128	31.320775449336786	21.460638882348324
39	20.345969704104856	26.296366062318498	31.964994898359627	21.392669335217015
40	19.491405698139864	26.87654030295895	31.56879365826858	22.063260340632603
41	20.716898202652853	25.945844125264895	31.443371791853075	21.89388588022918
42	19.518719095832353	27.6680009418413	30.876697276508906	21.93658268581744
43	19.988226983753236	27.396122753316064	30.113020955968917	22.502629306961776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	185.0
1	211.5
2	238.0
3	443.0
4	648.0
5	648.0
6	1048.5
7	1449.0
8	1566.0
9	1683.0
10	2531.0
11	3379.0
12	3379.0
13	6003.5
14	8628.0
15	12955.0
16	17282.0
17	16097.0
18	14912.0
19	14912.0
20	15843.5
21	16775.0
22	13694.5
23	10614.0
24	11670.0
25	12726.0
26	12726.0
27	14156.0
28	15586.0
29	16788.5
30	17991.0
31	19631.5
32	21272.0
33	21272.0
34	23312.5
35	25353.0
36	27357.0
37	29361.0
38	31025.5
39	32690.0
40	32690.0
41	33489.0
42	34288.0
43	35884.5
44	37481.0
45	39166.0
46	40851.0
47	40851.0
48	51256.0
49	61661.0
50	57698.5
51	53736.0
52	49390.0
53	45044.0
54	45044.0
55	40396.0
56	35748.0
57	30737.0
58	25726.0
59	23025.0
60	20324.0
61	20324.0
62	18064.0
63	15804.0
64	13778.0
65	11752.0
66	10021.0
67	8290.0
68	8290.0
69	7026.0
70	5762.0
71	4919.0
72	4076.0
73	3310.0
74	2544.0
75	2544.0
76	1991.0
77	1438.0
78	1143.0
79	848.0
80	641.5
81	435.0
82	435.0
83	338.5
84	242.0
85	174.5
86	107.0
87	84.0
88	61.0
89	61.0
90	50.0
91	39.0
92	25.5
93	12.0
94	8.5
95	5.0
96	5.0
97	3.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	637050.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.6134513553498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.57803080980545	59.00488346501651
2	6.967755913655011	9.282925392204177
3	1.7676177221327856	3.5324135144443964
4	0.7541972109659006	2.0095871690007003
5	0.43203674004203657	1.4389729183257052
6	0.2706602773498584	1.081776913144217
7	0.2090155586265248	0.9746273422955489
8	0.1498238704952577	0.7984228087284861
9	0.11929345131079955	0.715189366431336
>10	0.6666522627032716	8.138236930519723
>50	0.050242103680881944	2.3270465702699914
>100	0.02901300507184642	3.8690838409271247
>500	0.0033022932601978854	1.5223990145658455
>1k	0.0016511466300989427	1.8765622283992045
>5k	7.07634270042404E-4	3.427872525726957
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8260	1.2966015226434346	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7372	1.1572090102817674	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6184	0.9707244329330508	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3335	0.5235067891060357	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1770	0.2778431834235931	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1598	0.250843732831018	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1483	0.23279177458598227	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1341	0.2105015304921121	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1250	0.19621693744604035	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1166	0.18303115924966645	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	904	0.14190408916097638	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	833	0.13075896711404128	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.1299740993642571	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	825	0.12950317871438663	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	775	0.12165450121654502	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	753	0.1182010831174947	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	714	0.11207911466917825	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	699	0.10972451141982575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.5697354995683227E-4	0.0	0.0	0.0	0.0
6	1.5697354995683227E-4	0.0	0.0	0.0	0.0
7	1.5697354995683227E-4	0.0	0.0	0.0	0.0
8	1.5697354995683227E-4	0.0	0.0	0.0	0.0
9	1.5697354995683227E-4	0.0	0.0	1.5697354995683227E-4	0.0
10	1.5697354995683227E-4	0.0	0.0	1.5697354995683227E-4	0.0
11	1.5697354995683227E-4	0.0	0.0	1.5697354995683227E-4	0.0
12	1.5697354995683227E-4	0.0	0.0	7.848677497841614E-4	0.0
13	1.5697354995683227E-4	0.0	0.0	0.0012557883996546582	0.0
14	4.709206498704968E-4	0.0	0.0	0.0015697354995683227	0.0
15	4.709206498704968E-4	0.0	0.0	0.0023546032493524842	0.0
16	4.709206498704968E-4	0.0	0.0	0.0031394709991366455	0.0
17	4.709206498704968E-4	3.1394709991366455E-4	0.0	0.004395259398791303	0.0
18	4.709206498704968E-4	6.278941998273291E-4	0.0	0.004866180048661801	0.0
19	4.709206498704968E-4	6.278941998273291E-4	0.0	0.005964994898359627	0.0
20	4.709206498704968E-4	6.278941998273291E-4	0.0	0.01036025429715093	0.0
21	4.709206498704968E-4	6.278941998273291E-4	0.0	0.01789498469507888	0.0
22	4.709206498704968E-4	6.278941998273291E-4	0.0	0.02762734479240248	0.0
23	4.709206498704968E-4	6.278941998273291E-4	0.0	0.03484812809041676	0.0
24	4.709206498704968E-4	7.848677497841614E-4	0.0	0.044580488187740366	0.0
25	4.709206498704968E-4	7.848677497841614E-4	0.0	0.05007456243622949	0.0
26	4.709206498704968E-4	7.848677497841614E-4	0.0	0.06121968448316459	0.0
27	4.709206498704968E-4	7.848677497841614E-4	0.0	0.10423043717133663	0.0
28	4.709206498704968E-4	7.848677497841614E-4	0.0	0.20265285299427047	0.0
29	4.709206498704968E-4	7.848677497841614E-4	0.0	0.3120634173141826	0.0
30	4.709206498704968E-4	7.848677497841614E-4	0.0	0.473589200219763	0.0
31	4.709206498704968E-4	7.848677497841614E-4	0.0	0.6828349423122204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACCG	25	0.005494527	29.6	5
AGTCGTG	35	8.865036E-4	26.428572	20
GGTATCA	2040	0.0	26.02696	1
TCTATAC	40	0.0019302479	23.125	3
CCGTTTA	65	2.6797297E-6	22.76923	27
CGCCGTT	60	3.722931E-5	21.583332	25
GCTAGTG	45	0.0038239644	20.555555	2
TAGGACA	45	0.0038239644	20.555555	4
GTATTGG	245	0.0	18.877552	1
GACCGGC	50	0.0070315236	18.5	7
GCCCGTG	70	1.2181147E-4	18.5	24
CTAATAC	130	6.9485395E-10	18.5	3
TTGGACC	280	0.0	17.839287	4
CGTTTAC	75	2.0654444E-4	17.266666	28
GCCGTTT	75	2.0654444E-4	17.266666	26
TAATACT	145	2.9704097E-9	16.586205	4
CGAGGCT	90	4.4409535E-5	16.444445	33
TTACGGC	90	4.4409535E-5	16.444445	18
GTATCAA	3245	0.0	16.362095	2
TATACTA	170	8.54925E-11	16.32353	5
>>END_MODULE