FastQCFastQC Report
Wed 25 May 2016
SRR2088650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555709
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT244560.9569164564510279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT214330.8386322542981224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165880.6490566805532242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96820.37883812280662627No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58290.2280776097748218No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG46710.1827672868859483No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA45940.17975442431043598No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41840.1637119092979678No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA41060.16065991863705922No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG35990.14082197934115345No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26050.10192866245726723No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC25940.10149825351790834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68250.023.2571431
CATACCG909.489668E-820.5555575
GTATTGG7650.016.4444451
ACGTTCG1951.8189894E-1216.12820422
CGAACTA3150.015.26984124
TTGGACC10500.015.152384
ATTGGAC8750.015.0114293
GTATCAA106150.014.9533682
GGACCCT10800.014.2175936
GACGGTA800.00630140113.8750017
CTTAGAC2304.0017767E-1113.6739133
TGGACCC11400.013.6315795
TTAGACC1652.59859E-713.4545454
CTAATAC5450.012.8990823
CGTTCGC2753.6379788E-1212.78181723
GACCCTC11850.012.4894527
GTGTTAT2859.094947E-1212.3333341
CGTATAC1050.002645095312.3333333
TAATACT6850.012.1532844
AATACTG6550.012.1450385
Produced by FastQC (version 0.11.5)