##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088650_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2555709
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350063328806215	31.0	31.0	34.0	30.0	34.0
2	31.5658046358173	31.0	31.0	34.0	30.0	34.0
3	31.64679899002586	31.0	31.0	34.0	30.0	34.0
4	35.39513770934015	37.0	35.0	37.0	33.0	37.0
5	35.247636174541	37.0	35.0	37.0	33.0	37.0
6	35.30030296876522	37.0	35.0	37.0	32.0	37.0
7	35.162104136269036	37.0	35.0	37.0	32.0	37.0
8	35.17796353184185	37.0	35.0	37.0	32.0	37.0
9	36.7495990349449	39.0	37.0	39.0	32.0	39.0
10	36.514029179378404	38.0	35.0	39.0	32.0	39.0
11	36.63261192882288	39.0	35.0	39.0	32.0	39.0
12	36.471216793461224	38.0	35.0	39.0	32.0	39.0
13	36.518631033501855	38.0	35.0	39.0	32.0	39.0
14	37.458023585627316	39.0	36.0	41.0	32.0	41.0
15	37.47220751658346	39.0	36.0	41.0	32.0	41.0
16	37.35247166246236	39.0	36.0	41.0	32.0	41.0
17	37.35603388335683	39.0	36.0	41.0	32.0	41.0
18	37.31120561848004	39.0	36.0	40.0	32.0	41.0
19	37.3612594391615	39.0	36.0	41.0	32.0	41.0
20	37.227840884858175	39.0	36.0	40.0	31.0	41.0
21	37.159903181465495	39.0	36.0	40.0	31.0	41.0
22	37.01653122479907	39.0	36.0	40.0	31.0	41.0
23	36.83220507499093	39.0	36.0	40.0	30.0	41.0
24	36.676264394733515	39.0	35.0	40.0	30.0	41.0
25	36.875119585210996	39.0	36.0	40.0	30.0	41.0
26	36.74502926585147	39.0	36.0	40.0	30.0	41.0
27	36.62065125567895	39.0	35.0	40.0	30.0	41.0
28	36.44925537297087	39.0	35.0	40.0	30.0	41.0
29	36.34841134104078	39.0	35.0	40.0	30.0	41.0
30	36.15378041866269	38.0	35.0	40.0	29.0	41.0
31	36.02484476910321	38.0	35.0	40.0	29.0	41.0
32	35.8523654297105	38.0	35.0	40.0	28.0	41.0
33	35.72783951537519	38.0	34.0	40.0	27.0	41.0
34	35.532906132896976	38.0	34.0	40.0	27.0	41.0
35	35.40651420016911	38.0	34.0	40.0	26.0	41.0
36	35.21932113554399	38.0	34.0	40.0	25.0	41.0
37	35.17216005421587	38.0	34.0	40.0	25.0	41.0
38	34.98480499931721	38.0	33.0	40.0	25.0	41.0
39	34.90480684616285	38.0	33.0	40.0	24.0	41.0
40	34.76314361298567	38.0	33.0	40.0	24.0	41.0
41	34.649571997437896	38.0	33.0	40.0	23.0	41.0
42	34.40412308287055	38.0	33.0	40.0	23.0	41.0
43	34.06227508687413	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	3.0
14	5.0
15	29.0
16	50.0
17	103.0
18	266.0
19	626.0
20	1269.0
21	2554.0
22	4623.0
23	7879.0
24	12228.0
25	18531.0
26	26504.0
27	35946.0
28	48596.0
29	62148.0
30	77589.0
31	95298.0
32	116270.0
33	140555.0
34	168997.0
35	207094.0
36	263091.0
37	352002.0
38	483028.0
39	430421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.39247034775869	22.235277960049444	13.82410908284159	23.548142609350283
2	19.238888308488956	24.06416379955621	34.024491833772935	22.672456058181897
3	20.077324922360095	25.136390723670026	32.09864659865423	22.68763775531565
4	14.31266235709934	17.17875548429027	36.387906447878066	32.12067571073233
5	12.743626132709164	38.945318109377865	35.02918368249281	13.281872075420168
6	32.27742282082976	38.01688689909532	14.126608311040107	15.579081969034817
7	26.22563836493122	32.891264224526346	22.06996179925023	18.81313561129221
8	26.76235831231177	34.90933435692405	19.483008433276243	18.84529889748794
9	24.968139956466093	14.403948180328824	21.393163306151052	39.23474855705403
10	15.760518901017292	28.219879493322598	34.46362633617521	21.555975269484907
11	33.35841443607234	22.748794952790007	23.438466585984553	20.4543240251531
12	20.541070990476616	27.258698075563377	31.43112146179397	20.769109472166043
13	30.783982057425163	21.351570151374823	26.20760031756354	21.65684747363647
14	20.47830954150101	22.8441892249861	27.84092398625978	28.83657724725311
15	22.834759356405602	29.93975448691537	25.06975559424019	22.155730562438837
16	21.861800384942104	28.62642030058978	26.608154527765095	22.903624786703027
17	21.594790330198	27.442795717352798	27.419123225688057	23.543290726761146
18	22.409280555806628	26.4949961047991	29.31660059889448	21.779122740499798
19	22.43044885000601	25.77061785985807	30.292924585702053	21.506008704433878
20	23.256912269745893	25.615083720407917	29.87621047623184	21.251793533614354
21	22.309895218900117	26.266527214170317	30.232315181423235	21.19126238550633
22	21.505813064006894	26.541715038762238	29.87304110131474	22.079430795916124
23	22.13714472187561	26.687897565802682	30.326026945947287	20.84893076637442
24	22.096881922002858	26.700379425044083	28.681630029083905	22.52110862386915
25	22.161325878650505	26.72236940903679	29.552621210004737	21.56368350230797
26	21.82396352636392	27.35886597417781	29.403464948474177	21.41370555098409
27	21.133078922522085	27.367004615940232	29.330021532185395	22.169894929352285
28	21.53781983786104	26.991374996136102	29.548199736354963	21.9226054296479
29	21.648630575703258	26.960737705270827	29.159501336028477	22.23113038299744
30	21.781470425623574	27.146791751330063	30.02998385183916	21.041753971207207
31	22.189419843965023	27.004443776658455	29.84827302325891	20.957863356117617
32	21.784483288199084	26.919027166238408	29.273872729641752	22.02261681592075
33	21.584577899909576	26.85325285468729	29.664801430835823	21.89736781456731
34	21.163755341472758	27.192532483158296	30.117356866529015	21.526355308839936
35	20.890876073919216	28.532317255211765	29.308618469473636	21.268188201395386
36	22.355909847326124	26.986953522486324	29.145884762310576	21.511251867876975
37	21.66925107670709	27.308038591248064	29.271525044517976	21.751185287526866
38	21.532576674417943	26.385359209518768	30.137664342849675	21.944399773213615
39	21.07630407061211	26.32040658776097	30.72102496802257	21.88226437360435
40	20.484413522822827	26.583190809282275	30.48907367779352	22.443321990101378
41	21.414370728435827	25.783334487611853	30.459492845233942	22.342801938718377
42	20.307241552148543	27.193275916780824	30.11903937420105	22.380443156869582
43	20.707600121923115	26.82758483066734	29.61346538279593	22.851349664613615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1251.0
1	997.0
2	743.0
3	1135.0
4	1527.0
5	1527.0
6	2620.0
7	3713.0
8	4057.0
9	4401.0
10	6698.0
11	8995.0
12	8995.0
13	16974.5
14	24954.0
15	38377.0
16	51800.0
17	48316.5
18	44833.0
19	44833.0
20	49630.5
21	54428.0
22	47209.5
23	39991.0
24	45414.5
25	50838.0
26	50838.0
27	57237.5
28	63637.0
29	69989.5
30	76342.0
31	84369.5
32	92397.0
33	92397.0
34	102002.5
35	111608.0
36	119620.5
37	127633.0
38	133130.5
39	138628.0
40	138628.0
41	142844.5
42	147061.0
43	152384.5
44	157708.0
45	163171.0
46	168634.0
47	168634.0
48	199271.0
49	229908.0
50	216832.0
51	203756.0
52	189455.5
53	175155.0
54	175155.0
55	160205.5
56	145256.0
57	127219.5
58	109183.0
59	99159.5
60	89136.0
61	89136.0
62	79240.0
63	69344.0
64	60552.0
65	51760.0
66	44409.5
67	37059.0
68	37059.0
69	31940.5
70	26822.0
71	22975.0
72	19128.0
73	15625.0
74	12122.0
75	12122.0
76	9695.5
77	7269.0
78	5654.5
79	4040.0
80	3153.0
81	2266.0
82	2266.0
83	1717.5
84	1169.0
85	902.0
86	635.0
87	486.0
88	337.0
89	337.0
90	242.0
91	147.0
92	98.0
93	49.0
94	41.0
95	33.0
96	33.0
97	22.5
98	12.0
99	6.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2555709.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.40686690638701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.54444510939199	44.62715350224876
2	11.396991052199423	12.629431327249943
3	3.435866083927999	5.711117244611033
4	1.4782102344012849	3.2761199086852453
5	0.7517015646503685	2.082471427295292
6	0.474810486976659	1.5784656874603527
7	0.30986338501310196	1.2017991532808445
8	0.2338716243529337	1.036647517096283
9	0.16679468748477255	0.8317413945145087
>10	1.0626639204397734	11.165914224440202
>50	0.0854665833960287	3.2627130899465526
>100	0.052795759230190656	5.411256861909656
>500	0.0038975322766389665	1.5541728932490746
>1k	0.0022676551427703866	2.568902556313627
>5k	1.4172844642314916E-4	0.6090184106100031
>10k+	2.1259266963472376E-4	2.4530748010883348
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24456	0.9569164564510279	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21433	0.8386322542981224	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16588	0.6490566805532242	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9682	0.37883812280662627	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5829	0.2280776097748218	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4671	0.1827672868859483	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4594	0.17975442431043598	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4184	0.1637119092979678	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4106	0.16065991863705922	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3599	0.14082197934115345	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2605	0.10192866245726723	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2594	0.10149825351790834	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	3.912808539626381E-5	0.0	0.0	3.912808539626381E-5	0.0
10	3.912808539626381E-5	0.0	0.0	1.1738425618879145E-4	0.0
11	7.825617079252763E-5	0.0	0.0	1.5651234158505525E-4	0.0
12	7.825617079252763E-5	0.0	0.0	1.9564042698131908E-4	0.0
13	7.825617079252763E-5	0.0	0.0	3.130246831701105E-4	0.0
14	1.1738425618879145E-4	0.0	0.0	3.9128085396263816E-4	0.0
15	1.1738425618879145E-4	0.0	0.0	5.477931955476935E-4	0.0
16	1.1738425618879145E-4	0.0	0.0	7.825617079252763E-4	0.0
17	1.1738425618879145E-4	0.0	0.0	0.0011347144764916506	0.0
18	1.1738425618879145E-4	0.0	0.0	0.001486867245058025	0.0
19	1.5651234158505525E-4	0.0	0.0	0.0023085570383795653	0.0
20	1.5651234158505525E-4	0.0	0.0	0.0036389119418525346	0.0
21	1.5651234158505525E-4	0.0	0.0	0.006573518346572321	0.0
22	1.5651234158505525E-4	0.0	0.0	0.010368942630009911	0.0
23	1.5651234158505525E-4	0.0	0.0	0.013029652436955851	0.0
24	1.5651234158505525E-4	0.0	0.0	0.017098973318167288	0.0
25	1.5651234158505525E-4	0.0	0.0	0.020620501003831032	0.0
26	1.5651234158505525E-4	0.0	0.0	0.026998378923422033	0.0
27	1.5651234158505525E-4	0.0	0.0	0.06565692729493068	0.0
28	1.5651234158505525E-4	0.0	0.0	0.14539996533251634	0.0
29	1.5651234158505525E-4	0.0	0.0	0.24560699203234798	0.0
30	1.5651234158505525E-4	0.0	0.0	0.3784859700380599	0.0
31	1.5651234158505525E-4	0.0	0.0	0.5349983116231152	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6825	0.0	23.257143	1
CATACCG	90	9.489668E-8	20.555557	5
GTATTGG	765	0.0	16.444445	1
ACGTTCG	195	1.8189894E-12	16.128204	22
CGAACTA	315	0.0	15.269841	24
TTGGACC	1050	0.0	15.15238	4
ATTGGAC	875	0.0	15.011429	3
GTATCAA	10615	0.0	14.953368	2
GGACCCT	1080	0.0	14.217593	6
GACGGTA	80	0.006301401	13.875001	7
CTTAGAC	230	4.0017767E-11	13.673913	3
TGGACCC	1140	0.0	13.631579	5
TTAGACC	165	2.59859E-7	13.454545	4
CTAATAC	545	0.0	12.899082	3
CGTTCGC	275	3.6379788E-12	12.781817	23
GACCCTC	1185	0.0	12.489452	7
GTGTTAT	285	9.094947E-12	12.333334	1
CGTATAC	105	0.0026450953	12.333333	3
TAATACT	685	0.0	12.153284	4
AATACTG	655	0.0	12.145038	5
>>END_MODULE