FastQCFastQC Report
Wed 25 May 2016
SRR2088649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572289
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT379121.4738623848253443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323841.2589565169388042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT255540.9934342525276125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133950.5207424204667516No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG84250.3275292939479195No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG82140.3193264831440013No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA72870.28328854183958335No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA69520.2702651218428412No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA64040.24896113928100616No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG62900.24452928889405504No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA43510.16914895643529945No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40040.1556590258715098No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC39910.155153639423875No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34820.1353658162049443No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33860.13163373166856446No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33650.13081733817623137No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.11981546397002825No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA30220.11748291113479084No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA28800.11196253609139564No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG26810.1042262358545249No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT26240.10201031066104935No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25790.10026089603462131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89400.024.335571
GTATTGG7850.020.7388531
ATTGGAC8700.018.074713
TTGGACC11750.017.1617034
TGGACCC11900.016.7899155
GGACCCT12150.016.2921816
CTAATAC4100.015.3414633
GTATCAA143850.015.17552952
TGCGACG1353.9774022E-715.07407522
CAATGCG1702.440538E-814.14705919
TATTGGA10350.014.1207732
TATACAG3200.013.8750015
TAGTCCG800.00630141513.8750015
TTCGTTA2405.456968E-1213.87499930
TCTAATA5100.013.7843132
GACCCTC13600.013.7389717
GTGCTAG2651.8189894E-1213.2641521
CGTTCGC2950.013.16949323
CGACGAG1551.8899082E-613.12903224
CGCGATA850.00940886713.05882414
Produced by FastQC (version 0.11.5)