FastQCFastQC Report
Wed 25 May 2016
SRR2088648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807582
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT413751.4736880347573107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT349761.2457694913274127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290831.035873573772734No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159450.5679264220955969No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG97290.3465259429644441No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG84960.30260914908273384No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA78380.2791726118774091No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA74970.26702692922237No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG70360.25060710604356345No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68440.2437684812055356No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA45520.16213239720157774No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44540.15864184910716767No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC44230.15753769613852775No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39660.14126034431051346No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA39340.14012057350417548No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.13627384703278478No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37210.1325339740744883No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG36050.1284023049015131No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA33360.11882110656073447No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC29720.10585621363863995No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA28560.10172454446566476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117750.021.5872611
GTATTGG9100.019.719781
CCGTTTA951.6782724E-719.47368427
TAGACCG500.00703734818.55
TTGGACC14050.016.4590744
ACCGTTA1352.222987E-816.4444458
ATTGGAC10850.016.3686643
AGTGCGC1557.221388E-915.5161298
CGTGTCT2550.015.23529435
GGACCCT15000.015.0466666
GTATTAG4200.014.976191
CGTTTAC1252.9622443E-614.79999928
TATTGGA12350.014.5303642
GTATCAA175350.014.4644992
CGCGATA908.2792604E-414.38888814
TGGACCC16500.014.3515165
TAACGGT1304.4491735E-614.2307694
CTAATAC4850.014.1134023
TACGACT1203.303693E-513.87500120
GCGCATA1501.307304E-613.56666726
Produced by FastQC (version 0.11.5)