FastQCFastQC Report
Wed 25 May 2016
SRR2088647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020599
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT322521.596160346511109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT270141.3369302865140484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT180460.8931015010895283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117130.5796795900621549No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64300.31822246769398577No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG63300.313273440202633No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA58430.2891716763197448No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA52480.25972496274619555No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG44650.22097407748890305No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42930.21246175020377622No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33580.1661883431596274No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29790.14743152896740025No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28470.14089881267881454No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA26420.13075330632154128No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24540.121449134637798No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA22750.11259037542827646No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT22300.1103633130571677No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21680.10729491601252897No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC20770.10279130099539789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83350.023.7714461
TTATGCG1850.020.04
TATGCGG2200.017.659095
CGATATA650.001580494317.0769232
GTATTGG5100.017.0490191
CGAATTA1452.9831426E-916.58620615
TATACCG1452.9831426E-916.5862065
GTATCAA121950.016.2624022
ACGGGTT1302.5916052E-715.6538465
AACGGAC855.366651E-415.23529426
CGACTTA855.366651E-415.23529424
ATTAGAC1954.1836756E-1115.1794863
TTGGACC8200.014.6646344
ATTGGAC6000.014.4916673
TGGACCC9000.014.3888895
GTATTAG3100.014.322581
ACGTTCG1304.447689E-614.23076922
TATACGG1304.447689E-614.2307692
GCCCCGA1956.730261E-1014.23076811
GGACCCT8650.013.9017356
Produced by FastQC (version 0.11.5)