##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2020599
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.979552598016728	31.0	31.0	33.0	30.0	34.0
2	31.19544501407751	31.0	31.0	34.0	28.0	34.0
3	31.208942991657423	31.0	31.0	34.0	28.0	34.0
4	34.99896416854606	35.0	35.0	37.0	32.0	37.0
5	34.834302105464765	35.0	35.0	37.0	32.0	37.0
6	34.97190288622334	35.0	35.0	37.0	32.0	37.0
7	34.81201119074096	35.0	35.0	37.0	32.0	37.0
8	34.8841922617996	35.0	35.0	37.0	32.0	37.0
9	36.37420487687067	38.0	35.0	39.0	32.0	39.0
10	36.07906170397986	38.0	35.0	39.0	31.0	39.0
11	36.26615424436021	38.0	35.0	39.0	32.0	39.0
12	36.04948978001078	38.0	35.0	39.0	31.0	39.0
13	36.14603738792309	38.0	35.0	39.0	32.0	39.0
14	36.91131837638245	38.0	36.0	40.0	31.0	41.0
15	36.964701556320676	38.0	36.0	40.0	31.0	41.0
16	36.80411501737851	38.0	36.0	40.0	31.0	41.0
17	36.868113366382936	38.0	36.0	40.0	31.0	41.0
18	36.841129783791835	38.0	36.0	40.0	31.0	41.0
19	36.911955811123335	39.0	36.0	40.0	31.0	41.0
20	36.781018400979114	39.0	36.0	40.0	30.0	41.0
21	36.72086891065471	39.0	35.0	40.0	30.0	41.0
22	36.5573871906301	38.0	35.0	40.0	30.0	41.0
23	36.35429345456471	38.0	35.0	40.0	30.0	41.0
24	36.196351675913924	38.0	34.0	40.0	29.0	41.0
25	36.36387576159347	38.0	35.0	40.0	30.0	41.0
26	36.1809963283165	38.0	35.0	40.0	30.0	41.0
27	36.03560577828654	38.0	35.0	40.0	29.0	41.0
28	35.83471485435754	38.0	34.0	40.0	28.0	41.0
29	35.70242289538894	38.0	34.0	40.0	27.0	41.0
30	35.45808445911336	38.0	34.0	40.0	27.0	41.0
31	35.277588477476236	38.0	34.0	40.0	27.0	41.0
32	35.08697618874403	38.0	34.0	40.0	26.0	41.0
33	34.9306042416135	37.0	33.0	40.0	25.0	41.0
34	34.70579021369406	37.0	33.0	40.0	25.0	41.0
35	34.53972064719422	37.0	33.0	40.0	25.0	41.0
36	34.3380457973106	37.0	33.0	40.0	24.0	41.0
37	34.23426963984442	37.0	33.0	40.0	24.0	41.0
38	33.99085122777949	37.0	33.0	40.0	22.0	41.0
39	33.855322109928785	37.0	33.0	40.0	21.0	41.0
40	33.63161171513992	37.0	32.0	40.0	20.0	41.0
41	33.466760104305706	37.0	32.0	40.0	19.0	41.0
42	33.14770619999317	37.0	31.0	40.0	18.0	41.0
43	32.75680874829692	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	9.0
15	35.0
16	68.0
17	153.0
18	348.0
19	797.0
20	1603.0
21	3003.0
22	5231.0
23	8441.0
24	13426.0
25	19353.0
26	27337.0
27	36306.0
28	46893.0
29	59811.0
30	72931.0
31	88263.0
32	105038.0
33	126280.0
34	150777.0
35	182604.0
36	235164.0
37	303010.0
38	353976.0
39	179735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.03867120591468	24.307890877903038	15.627247167795291	21.026190748386988
2	21.26735685804061	25.77097187517167	31.92424622599536	21.03742504079236
3	22.353123999368503	25.00159606136596	32.404202912106754	20.241077027158777
4	16.186685235417812	18.298385775703146	34.02639514322238	31.488533845656658
5	12.32911626700795	40.46062578473017	33.91528947604151	13.294968472220367
6	32.33466907585325	38.02001287736953	12.916169908032222	16.729148138744996
7	25.23286411603688	34.17714252060899	21.096021526289977	19.49397183706416
8	27.699508908002034	34.999472928572175	18.55553724415384	18.74548091927196
9	24.633437906284225	14.127345405990996	21.764486669547	39.47473001817778
10	16.37459980926448	29.208566370665334	33.13032422563804	21.286509594432147
11	33.42261378927734	22.63640633297354	22.996101651045063	20.944878226704063
12	20.43646463251739	28.45215700888697	31.080733980369185	20.030644378226455
13	32.34026147691848	20.98862762972762	26.32026443643692	20.350846456916983
14	19.883856222832932	23.459479095060427	28.703716076272435	27.95294860583421
15	22.55816220833525	30.428353176459062	25.822837683281048	21.190646931924643
16	20.98105561766585	28.564648403765418	27.629579149549215	22.824716829019515
17	21.45249997649212	27.419146500616897	27.872972321573947	23.255381201317036
18	22.35698424081176	25.700497723694802	30.431965966527745	21.510552068965687
19	22.251322503871375	24.95839105136645	32.048664777127975	20.741621667634202
20	23.079047351800135	24.76617082360231	31.64903080720123	20.505751017396324
21	22.029259640334374	25.587362955242483	32.31121068554424	20.072166718878908
22	20.6699102592845	25.959034919843077	31.870004884690132	21.50104993618229
23	21.591023255975085	26.211385831627155	32.46542238217479	19.732168530222967
24	21.479076254120685	26.161252183139755	30.360353538727875	21.99931802401169
25	21.500159111233845	26.566775495781204	31.204806099577404	20.72825929340755
26	21.089884732200698	27.284285501477534	31.26018571720564	20.365644049116128
27	20.347283157123208	27.073357949796073	31.03490598579926	21.544452907281457
28	20.683767536260287	27.041931625225985	31.102014798582005	21.172286039931723
29	20.843967556155377	26.467349533479922	30.705746167349385	21.982936743015312
30	21.04806544989877	26.929638191447193	32.0396080568188	19.982688301835246
31	21.720044402674652	26.855650230451467	31.512388158164978	19.9119172087089
32	21.282599862713976	26.740486360727683	30.765233477795444	21.211680298762893
33	20.933149031549554	26.743851699421807	31.17214251813447	21.150856750894164
34	20.22439880451292	27.27602062556697	31.827443248264498	20.672137321655608
35	19.806750374517655	28.972101837128495	30.87416157288012	20.346986215473727
36	21.99382460349629	26.826351987702658	30.546832894602044	20.632990514199008
37	21.106661935396385	27.601023260924112	30.545447166904466	20.746867636775036
38	21.037672492166926	26.092807132934343	31.686494945310773	21.18302542958796
39	20.371533391830837	26.114879795545775	32.37945777464999	21.13412903797339
40	19.422260428714456	26.55633304777445	31.92632481754173	22.09508170596937
41	20.713758642857886	25.6609549940389	31.90261897585815	21.72266738724507
42	19.79457576688893	27.37757466968953	31.136657991021476	21.691191572400065
43	20.063456430494124	27.247415246666957	30.40088607388205	22.28824224895687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1027.0
1	792.0
2	557.0
3	803.0
4	1049.0
5	1049.0
6	2036.0
7	3023.0
8	3563.5
9	4104.0
10	6687.5
11	9271.0
12	9271.0
13	18838.0
14	28405.0
15	46174.5
16	63944.0
17	56310.5
18	48677.0
19	48677.0
20	53786.5
21	58896.0
22	47225.5
23	35555.0
24	38518.0
25	41481.0
26	41481.0
27	44957.0
28	48433.0
29	51509.5
30	54586.0
31	59552.5
32	64519.0
33	64519.0
34	71179.5
35	77840.0
36	84230.5
37	90621.0
38	94997.5
39	99374.0
40	99374.0
41	102900.5
42	106427.0
43	111032.5
44	115638.0
45	123474.5
46	131311.0
47	131311.0
48	168960.0
49	206609.0
50	190705.0
51	174801.0
52	157675.5
53	140550.0
54	140550.0
55	124830.0
56	109110.0
57	94826.0
58	80542.0
59	71577.5
60	62613.0
61	62613.0
62	55404.5
63	48196.0
64	42022.0
65	35848.0
66	30803.0
67	25758.0
68	25758.0
69	22077.5
70	18397.0
71	15728.0
72	13059.0
73	10867.0
74	8675.0
75	8675.0
76	6980.0
77	5285.0
78	4130.0
79	2975.0
80	2313.5
81	1652.0
82	1652.0
83	1267.0
84	882.0
85	694.5
86	507.0
87	360.0
88	213.0
89	213.0
90	164.0
91	115.0
92	81.0
93	47.0
94	31.5
95	16.0
96	16.0
97	12.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2020599.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.96993012257565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.83872245766538	43.349914099033974
2	10.394731262396444	11.012163772241843
3	3.1913249451998102	5.071327780369995
4	1.380481905186975	2.9249612021293667
5	0.7672608121235299	2.0320875801987013
6	0.4511413184206334	1.4338154467288562
7	0.313218863943596	1.161382693531537
8	0.22403064762226807	0.9493510199893527
9	0.18492387145824735	0.8815864094225572
>10	1.0853769433718414	10.8584903821573
>50	0.09916254544944461	3.6216974391403887
>100	0.06082907372236108	5.950250307695782
>500	0.004397676719833368	1.6425570074078757
>1k	0.0036491360015638583	3.515977010516119
>5k	3.742703591347547E-4	1.1821196458672112
>10k+	3.742703591347547E-4	4.412318203569178
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32252	1.596160346511109	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27014	1.3369302865140484	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18046	0.8931015010895283	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11713	0.5796795900621549	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6430	0.31822246769398577	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6330	0.313273440202633	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5843	0.2891716763197448	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5248	0.25972496274619555	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4465	0.22097407748890305	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4293	0.21246175020377622	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3358	0.1661883431596274	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2979	0.14743152896740025	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2847	0.14089881267881454	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2642	0.13075330632154128	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2454	0.121449134637798	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2275	0.11259037542827646	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2230	0.1103633130571677	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2168	0.10729491601252897	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2077	0.10279130099539789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.898054982705623E-5	0.0
10	0.0	0.0	0.0	1.4847082474058436E-4	0.0
11	0.0	0.0	0.0	1.9796109965411246E-4	0.0
12	0.0	0.0	0.0	2.969416494811687E-4	0.0
13	0.0	0.0	0.0	2.969416494811687E-4	0.0
14	0.0	0.0	0.0	3.464319243946968E-4	0.0
15	0.0	0.0	0.0	7.918443986164498E-4	0.0
16	0.0	0.0	0.0	9.403152233570342E-4	0.0
17	0.0	0.0	0.0	0.0018806304467140684	0.0
18	0.0	0.0	0.0	0.0022270623711087654	0.0
19	4.9490274913528115E-5	0.0	0.0	0.002919926219898159	0.0
20	4.9490274913528115E-5	0.0	0.0	0.004949027491352812	0.0
21	4.9490274913528115E-5	0.0	0.0	0.00816589536073214	0.0
22	4.9490274913528115E-5	0.0	0.0	0.012521039553122613	0.0
23	4.9490274913528115E-5	0.0	0.0	0.015341985223193716	0.0
24	4.9490274913528115E-5	0.0	0.0	0.020241522439633	0.0
25	4.9490274913528115E-5	0.0	0.0	0.02444819580728289	0.0
26	4.9490274913528115E-5	0.0	0.0	0.033306955016804425	0.0
27	4.9490274913528115E-5	0.0	0.0	0.07661094556614152	0.0
28	4.9490274913528115E-5	0.0	0.0	0.16321892666481572	0.0
29	4.9490274913528115E-5	0.0	0.0	0.28343080442977553	0.0
30	4.9490274913528115E-5	9.898054982705623E-5	0.0	0.4527370349089552	0.0
31	4.9490274913528115E-5	9.898054982705623E-5	0.0	0.6421858072779408	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8335	0.0	23.771446	1
TTATGCG	185	0.0	20.0	4
TATGCGG	220	0.0	17.65909	5
CGATATA	65	0.0015804943	17.076923	2
GTATTGG	510	0.0	17.049019	1
CGAATTA	145	2.9831426E-9	16.586206	15
TATACCG	145	2.9831426E-9	16.586206	5
GTATCAA	12195	0.0	16.262402	2
ACGGGTT	130	2.5916052E-7	15.653846	5
AACGGAC	85	5.366651E-4	15.235294	26
CGACTTA	85	5.366651E-4	15.235294	24
ATTAGAC	195	4.1836756E-11	15.179486	3
TTGGACC	820	0.0	14.664634	4
ATTGGAC	600	0.0	14.491667	3
TGGACCC	900	0.0	14.388889	5
GTATTAG	310	0.0	14.32258	1
ACGTTCG	130	4.447689E-6	14.230769	22
TATACGG	130	4.447689E-6	14.230769	2
GCCCCGA	195	6.730261E-10	14.230768	11
GGACCCT	865	0.0	13.901735	6
>>END_MODULE