FastQCFastQC Report
Wed 25 May 2016
SRR2088646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735187
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT251051.446818123925548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT233351.3448118271978755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136910.7890215867223533No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96790.5578073141396287No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG50790.2927062039999147No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG49550.2855600001613659No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA46130.26585030892923933No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA33840.1950222079810418No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32890.18954729374989554No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG30160.1738141191698647No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28010.16142352380463892No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.1540467972616208No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC21320.12286860148214573No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.12263807877767642No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18690.10771173366328815No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT18050.1040233703917791No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA17930.10333180227837115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67100.024.5380021
TTATGCG1554.0199666E-1016.7096794
GTATCAA98800.016.6462552
CGAACTA2050.016.24390224
CCGGTCT2950.013.796619
TCGAACC2900.013.39655131
TACGGCT2102.042725E-913.2142874
TATGCGG2102.042725E-913.2142875
GTATTGG4650.013.1290331
ATTAGAG2551.6370905E-1113.0588243
CCGTAGG850.00940725513.0588241
GTGCTAT1857.388553E-813.01
CGAACCA1153.578805E-412.86956414
ATTGGAC6400.012.718753
GTATTAG3500.012.6857151
TCACGTA2951.8189894E-1212.54237325
ACGTTCG1205.123785E-412.33333322
TATGGGC1359.965716E-512.3333333
CACGTAG3001.8189894E-1212.33333326
TACTCCG4100.012.1829265
Produced by FastQC (version 0.11.5)