##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088646_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1735187
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.839591928708547	31.0	31.0	31.0	28.0	34.0
2	31.047720505052194	31.0	31.0	34.0	28.0	34.0
3	31.03796536050581	31.0	31.0	34.0	27.0	34.0
4	34.8309911266048	35.0	35.0	37.0	32.0	37.0
5	34.63553380701907	35.0	35.0	37.0	32.0	37.0
6	34.81928057321776	35.0	35.0	37.0	32.0	37.0
7	34.68015378169615	35.0	35.0	37.0	31.0	37.0
8	34.74893829886923	35.0	35.0	37.0	32.0	37.0
9	36.238438854140796	38.0	35.0	39.0	32.0	39.0
10	35.9033228118929	37.0	35.0	39.0	30.0	39.0
11	36.124370456901765	38.0	35.0	39.0	32.0	39.0
12	35.88699719396238	37.0	35.0	39.0	30.0	39.0
13	36.01304527984592	37.0	35.0	39.0	31.0	39.0
14	36.76057739021788	38.0	36.0	40.0	31.0	41.0
15	36.81519167674723	38.0	36.0	40.0	31.0	41.0
16	36.63069513545226	38.0	36.0	40.0	30.0	41.0
17	36.69308552911012	38.0	36.0	40.0	31.0	41.0
18	36.65951450765825	38.0	36.0	40.0	31.0	41.0
19	36.73340279750828	38.0	36.0	40.0	30.0	41.0
20	36.595976110932135	38.0	35.0	40.0	30.0	41.0
21	36.53241408562881	38.0	35.0	40.0	30.0	41.0
22	36.3640005371179	38.0	35.0	40.0	30.0	41.0
23	36.16431485482545	38.0	34.0	40.0	29.0	41.0
24	36.00622929978152	38.0	34.0	40.0	29.0	41.0
25	36.169191562638495	38.0	34.0	40.0	30.0	41.0
26	35.97568331251905	38.0	34.0	40.0	29.0	41.0
27	35.8194402101906	38.0	34.0	40.0	28.0	41.0
28	35.619130387675796	38.0	34.0	40.0	27.0	41.0
29	35.49825984173464	38.0	34.0	40.0	27.0	41.0
30	35.26874336887033	38.0	34.0	40.0	27.0	41.0
31	35.091615485823716	38.0	34.0	40.0	26.0	41.0
32	34.885712029884964	37.0	33.0	40.0	25.0	41.0
33	34.736409966188084	37.0	33.0	40.0	25.0	41.0
34	34.507904911689636	37.0	33.0	40.0	25.0	41.0
35	34.34487752616865	37.0	33.0	40.0	24.0	41.0
36	34.13016637399888	37.0	33.0	40.0	24.0	41.0
37	34.046168510944355	37.0	33.0	40.0	23.0	41.0
38	33.8071873521413	37.0	33.0	40.0	22.0	41.0
39	33.66422812065789	37.0	32.0	40.0	21.0	41.0
40	33.465756716711226	37.0	32.0	40.0	20.0	41.0
41	33.30720608211104	37.0	31.0	40.0	19.0	41.0
42	33.00476490430138	37.0	31.0	40.0	17.0	41.0
43	32.604975717314616	36.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	6.0
14	9.0
15	35.0
16	63.0
17	157.0
18	332.0
19	701.0
20	1467.0
21	2846.0
22	4885.0
23	7931.0
24	12058.0
25	17611.0
26	24385.0
27	32908.0
28	41973.0
29	53375.0
30	65978.0
31	79230.0
32	94160.0
33	112869.0
34	134921.0
35	164612.0
36	210391.0
37	264974.0
38	285339.0
39	121966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.73997442350594	25.09671868219391	15.650071145069667	20.513235749230486
2	22.368770628180133	25.9776611973234	30.936492723838988	20.71707545065748
3	23.331433442044	25.30182625849548	31.616246548642884	19.750493750817636
4	17.384351081468452	19.112867950255506	33.44094901586976	30.06183195240628
5	12.311468446916672	40.777449346957994	33.98705730275757	12.924024903367764
6	32.15163552977287	38.320307840019545	13.176965940846722	16.35109068936086
7	25.103058056566812	33.99477981335729	21.6220499577279	19.280112172347994
8	27.462285044781915	35.4015446173813	18.807944042918717	18.328226294918068
9	24.64547048819522	14.003793251102042	21.68982363284188	39.66091262786086
10	16.25202355711517	29.289753784462423	33.50440039027494	20.95382226814747
11	33.5850833368392	22.59560496937794	23.032848909080116	20.786462784702746
12	19.86425670547324	28.46707588288755	31.536024647487558	20.132642764151644
13	31.88088661337366	21.112537150174592	26.60577793632617	20.40079830012558
14	19.75596866504878	23.375290386569286	28.789692407792362	28.079048540589575
15	22.74538709660688	30.329180658914574	25.86476270280955	21.060669541668997
16	21.00770695031717	28.571906082744974	27.885812883568168	22.534574083369687
17	21.249467636629365	27.65967011048377	28.299024831329415	22.791837421557446
18	21.978841473570284	25.807823594805633	30.447093022250627	21.766241909373456
19	22.097964081104806	25.195209507678424	32.01533898075539	20.691487430461386
20	22.76394417431666	24.947397600373908	31.822506738466807	20.46615148684263
21	21.841738095087155	25.700111861142343	32.30354999201815	20.154600051752347
22	20.634663583809697	25.939855473790434	31.993900369239743	21.431580573160126
23	21.441032004043368	26.19608145980808	32.56928503959516	19.793601496553396
24	21.412274296660822	26.314281976524722	30.565639323023973	21.707804403790483
25	21.419017085766548	26.631942263283438	31.336161462712663	20.612879188237347
26	21.144810328800297	27.188251179844013	31.31051581184045	20.356422679515234
27	20.484881456580762	27.01530152081591	30.99936779148299	21.500449231120335
28	20.609306086318075	27.063480766049995	31.432923367913663	20.894289779718267
29	20.94512003605375	26.316817726273882	30.973722140610782	21.764340097061584
30	21.153051515485075	26.705479006009153	31.961511929261803	20.17995754924397
31	21.46212483150231	26.818550392551348	31.621836724226267	20.097488051720074
32	21.133975761690238	26.65113327843051	31.077226834917504	21.13766412496175
33	20.894750825127208	26.660008402552577	31.38341861712887	21.061822155191344
34	20.349276475676685	27.13903458243982	31.87114702910983	20.640541912773667
35	19.96528328070692	28.544646772941473	30.9681319650274	20.521937981324204
36	21.936540557300162	26.675972099837082	30.606326580362808	20.78116076249995
37	21.029030300480585	27.40615276624364	30.673639210067847	20.89117772320793
38	21.084932056314393	26.026359118642546	31.638030944215235	21.25067788082783
39	20.397743874291358	26.051889508162525	32.26902921702387	21.281337400522247
40	19.663644321908823	26.48256355078732	31.76867968697322	22.08511244033064
41	20.857406147003175	25.785117108415406	31.603279646516487	21.754197098064935
42	20.183415389811014	27.05944661872179	30.844398903403498	21.912739088063706
43	20.38927216490211	26.909088184731672	30.322726023189432	22.378913627176782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	750.0
1	611.5
2	473.0
3	647.0
4	821.0
5	821.0
6	1661.0
7	2501.0
8	2909.0
9	3317.0
10	5348.5
11	7380.0
12	7380.0
13	15356.0
14	23332.0
15	37189.5
16	51047.0
17	44910.5
18	38774.0
19	38774.0
20	44646.5
21	50519.0
22	40394.0
23	30269.0
24	32997.5
25	35726.0
26	35726.0
27	39226.0
28	42726.0
29	46364.0
30	50002.0
31	54601.5
32	59201.0
33	59201.0
34	65330.0
35	71459.0
36	76920.5
37	82382.0
38	86103.5
39	89825.0
40	89825.0
41	92147.0
42	94469.0
43	98458.5
44	102448.0
45	107685.0
46	112922.0
47	112922.0
48	141313.5
49	169705.0
50	157398.5
51	145092.0
52	131326.0
53	117560.0
54	117560.0
55	104981.0
56	92402.0
57	80584.5
58	68767.0
59	61946.0
60	55125.0
61	55125.0
62	48674.0
63	42223.0
64	36577.0
65	30931.0
66	26493.0
67	22055.0
68	22055.0
69	18649.5
70	15244.0
71	12855.5
72	10467.0
73	8480.0
74	6493.0
75	6493.0
76	5245.5
77	3998.0
78	3107.0
79	2216.0
80	1728.0
81	1240.0
82	1240.0
83	931.5
84	623.0
85	476.0
86	329.0
87	261.0
88	193.0
89	193.0
90	153.0
91	113.0
92	75.5
93	38.0
94	28.5
95	19.0
96	19.0
97	13.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1735187.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.56087535918295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.74964607478289	49.6301346052023
2	9.26555926363488	10.851985223416904
3	2.451385168992783	4.306657840162079
4	1.0482508358413762	2.4554594617146472
5	0.550197314989879	1.611001819303971
6	0.3565809108973067	1.2529014167112658
7	0.2551027857699019	1.0457329708876102
8	0.17511739278762728	0.8204022249809059
9	0.13817320565812138	0.7282389487071598
>10	0.878512996788059	9.825282512657763
>50	0.07604976503485988	3.057442520560071
>100	0.048139804437449536	5.19077033005563
>500	0.0039375569647852205	1.5834970830465644
>1k	0.002854728799469285	3.208096260097723
>5k	1.9687784823926106E-4	0.8507499345298772
>10k+	2.953167723588916E-4	3.5816468479655645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25105	1.446818123925548	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23335	1.3448118271978755	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13691	0.7890215867223533	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9679	0.5578073141396287	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5079	0.2927062039999147	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4955	0.2855600001613659	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4613	0.26585030892923933	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3384	0.1950222079810418	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3289	0.18954729374989554	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3016	0.1738141191698647	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2801	0.16142352380463892	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2673	0.1540467972616208	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2132	0.12286860148214573	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2128	0.12263807877767642	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1869	0.10771173366328815	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1805	0.1040233703917791	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1793	0.10333180227837115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.763067611732914E-5	0.0
5	0.0	0.0	0.0	5.763067611732914E-5	0.0
6	0.0	0.0	0.0	5.763067611732914E-5	0.0
7	0.0	0.0	0.0	5.763067611732914E-5	0.0
8	0.0	0.0	0.0	5.763067611732914E-5	0.0
9	0.0	0.0	0.0	1.1526135223465828E-4	0.0
10	0.0	0.0	0.0	1.1526135223465828E-4	0.0
11	5.763067611732914E-5	0.0	0.0	2.881533805866457E-4	0.0
12	5.763067611732914E-5	0.0	0.0	4.0341473282130396E-4	0.0
13	5.763067611732914E-5	0.0	0.0	4.0341473282130396E-4	0.0
14	1.1526135223465828E-4	0.0	0.0	4.0341473282130396E-4	0.0
15	1.1526135223465828E-4	0.0	0.0	6.915681134079497E-4	0.0
16	1.1526135223465828E-4	0.0	0.0	0.0013255055506985703	0.0
17	1.7289202835198744E-4	0.0	0.0	0.0016136589312852158	0.0
18	1.7289202835198744E-4	0.0	0.0	0.0018441816357545324	0.0
19	1.7289202835198744E-4	0.0	0.0	0.002823903129749128	0.0
20	1.7289202835198744E-4	0.0	0.0	0.004379931384917015	0.0
21	2.3052270446931655E-4	0.0	0.0	0.007549618571370117	0.0
22	2.3052270446931655E-4	0.0	0.0	0.012505856717460424	0.0
23	2.3052270446931655E-4	0.0	0.0	0.016655265397908122	0.0
24	2.3052270446931655E-4	0.0	0.0	0.02201491827681973	0.0
25	2.3052270446931655E-4	0.0	0.0	0.025703281548328797	0.0
26	2.3052270446931655E-4	0.0	0.0	0.03261896268240829	0.0
27	2.3052270446931655E-4	0.0	0.0	0.07169256108995745	0.0
28	2.3052270446931655E-4	0.0	0.0	0.15427731996609012	0.0
29	2.3052270446931655E-4	0.0	0.0	0.2533444522117789	0.0
30	2.3052270446931655E-4	0.0	0.0	0.3925225350351288	0.0
31	2.3052270446931655E-4	0.0	0.0	0.5419588782073632	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6710	0.0	24.538002	1
TTATGCG	155	4.0199666E-10	16.709679	4
GTATCAA	9880	0.0	16.646255	2
CGAACTA	205	0.0	16.243902	24
CCGGTCT	295	0.0	13.79661	9
TCGAACC	290	0.0	13.396551	31
TACGGCT	210	2.042725E-9	13.214287	4
TATGCGG	210	2.042725E-9	13.214287	5
GTATTGG	465	0.0	13.129033	1
ATTAGAG	255	1.6370905E-11	13.058824	3
CCGTAGG	85	0.009407255	13.058824	1
GTGCTAT	185	7.388553E-8	13.0	1
CGAACCA	115	3.578805E-4	12.869564	14
ATTGGAC	640	0.0	12.71875	3
GTATTAG	350	0.0	12.685715	1
TCACGTA	295	1.8189894E-12	12.542373	25
ACGTTCG	120	5.123785E-4	12.333333	22
TATGGGC	135	9.965716E-5	12.333333	3
CACGTAG	300	1.8189894E-12	12.333333	26
TACTCCG	410	0.0	12.182926	5
>>END_MODULE