FastQCFastQC Report
Wed 25 May 2016
SRR2088645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211208
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123551.0200560101980833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109750.9061201709367839No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81170.6701573965825853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47740.39415195408220555No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG22990.18981050323313584No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG22740.18774644817405434No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA21640.17866460591409566No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA21330.1761051776408346No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG18250.15067601931295038No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17980.14844683984914236No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.11236715741639752No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.10848673390532428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT400.001931247323.1256
GGTATCA34900.022.5286541
TTGACGC450.003825925520.55555511
GTATTGG2400.019.2708341
TCGTCCG500.007035095218.510
TCTATCG609.237205E-418.531
TTATGCG1351.1514203E-917.8148164
TTAACGG1452.9795046E-916.58620835
GTATTAG2700.016.4444451
TATTGCG904.4462748E-516.44444511
TATGCGG1504.671165E-916.0333335
TATACTG1504.671165E-916.0333335
CGCCTAT700.002592600615.85714336
TAGGCGA700.002592600615.85714333
TATCGCC700.002592600615.85714333
CGATAGA700.002592600615.85714337
ATAGGCG957.060174E-515.57894832
GTATCAA52300.015.0688342
TCGCCTA750.00410457514.835
GCGCCAA750.00410457514.813
Produced by FastQC (version 0.11.5)