##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088645_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211208
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323242580960496	31.0	31.0	34.0	30.0	34.0
2	31.53926493219992	31.0	31.0	34.0	30.0	34.0
3	31.614591383148063	31.0	31.0	34.0	30.0	34.0
4	35.36604282666561	37.0	35.0	37.0	33.0	37.0
5	35.221812438491156	37.0	35.0	37.0	33.0	37.0
6	35.2783014973481	37.0	35.0	37.0	32.0	37.0
7	35.140937807544205	37.0	35.0	37.0	32.0	37.0
8	35.169904756243355	37.0	35.0	37.0	32.0	37.0
9	36.736203855985096	39.0	35.0	39.0	32.0	39.0
10	36.503473391853426	38.0	35.0	39.0	32.0	39.0
11	36.62334793033071	38.0	35.0	39.0	32.0	39.0
12	36.461491337573726	38.0	35.0	39.0	32.0	39.0
13	36.51860456668054	38.0	35.0	39.0	32.0	39.0
14	37.458718073196344	39.0	36.0	41.0	32.0	41.0
15	37.47152264516087	39.0	36.0	41.0	32.0	41.0
16	37.35475244549244	39.0	36.0	41.0	32.0	41.0
17	37.36601888362692	39.0	36.0	41.0	32.0	41.0
18	37.32293132145759	39.0	36.0	40.0	32.0	41.0
19	37.380146102073304	39.0	36.0	41.0	32.0	41.0
20	37.249069523979365	39.0	36.0	40.0	31.0	41.0
21	37.17896017859856	39.0	36.0	40.0	31.0	41.0
22	37.035840252045894	39.0	36.0	40.0	31.0	41.0
23	36.86687257679936	39.0	36.0	40.0	30.0	41.0
24	36.72079609778007	39.0	35.0	40.0	30.0	41.0
25	36.90598724579098	39.0	36.0	40.0	31.0	41.0
26	36.77026489257006	39.0	36.0	40.0	30.0	41.0
27	36.64818511766765	39.0	35.0	40.0	30.0	41.0
28	36.476511879049674	39.0	35.0	40.0	30.0	41.0
29	36.363771540478595	39.0	35.0	40.0	30.0	41.0
30	36.17831123968798	38.0	35.0	40.0	29.0	41.0
31	36.05369680517302	38.0	35.0	40.0	29.0	41.0
32	35.86888131518286	38.0	35.0	40.0	29.0	41.0
33	35.74227135223678	38.0	34.0	40.0	28.0	41.0
34	35.565578331715116	38.0	34.0	40.0	27.0	41.0
35	35.43964207634031	38.0	34.0	40.0	27.0	41.0
36	35.255392137436345	38.0	34.0	40.0	26.0	41.0
37	35.22241596819043	38.0	34.0	40.0	25.0	41.0
38	35.024543265896526	38.0	34.0	40.0	25.0	41.0
39	34.952601039623254	38.0	33.0	40.0	24.0	41.0
40	34.80293227917913	38.0	33.0	40.0	24.0	41.0
41	34.69797095131472	38.0	33.0	40.0	24.0	41.0
42	34.4496469640227	38.0	33.0	40.0	23.0	41.0
43	34.10415882325744	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	1.0
15	8.0
16	20.0
17	53.0
18	123.0
19	266.0
20	613.0
21	1274.0
22	2171.0
23	3676.0
24	5763.0
25	8655.0
26	12404.0
27	17184.0
28	22780.0
29	29072.0
30	36492.0
31	44627.0
32	54889.0
33	66357.0
34	79747.0
35	97959.0
36	124809.0
37	167480.0
38	232545.0
39	202236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14596006631396	23.07481456529349	14.876800681633542	22.902424686759005
2	19.279595247059135	23.91521522314912	34.8345618589045	21.970627670887247
3	20.05889987516595	25.26114424607499	32.08202059431576	22.5979352844433
4	14.756590114992635	17.58294198849413	35.91175091313796	31.748716983375274
5	12.792270196365942	38.869046439587585	34.916050752637034	13.422632611409435
6	32.00515518391556	38.18196379152053	14.186498107674323	15.626382916889584
7	25.65562644896665	33.17241960092734	22.241349132436376	18.930604817669632
8	25.98265533252753	35.686934036102805	19.496898963679236	18.833511667690438
9	24.509167706950414	14.924439072397144	21.864452678648092	38.70194054200435
10	16.18078810575888	28.28382903679632	34.2082449917768	21.327137865667993
11	33.06830866374727	23.15506502599058	23.217977424191385	20.558648886070767
12	20.34910601647281	27.793657241365644	31.208182244503007	20.649054497658536
13	30.474039141088898	21.475171894505323	26.37548629137192	21.675302673033865
14	20.15335103466952	23.172403088486863	28.212495293954465	28.46175058288915
15	22.640207132053288	30.177228023592974	25.327854505584508	21.85471033876923
16	21.52297540967365	28.76136881526542	26.963824545412514	22.751831229648417
17	21.43471641534732	27.692435981268286	27.629771269674574	23.243076333709816
18	22.159529989894384	26.612852623166294	29.55867200348743	21.66894538345189
19	22.355697782709495	25.881929445644346	30.5935892101109	21.16878356153526
20	22.926037476634896	25.807375776910323	30.26119378339641	21.005392963058366
21	22.05426318188123	26.419904756243355	30.650226880932095	20.875605180943325
22	21.20544117938455	26.764106577895785	30.20546429680121	21.824987945918455
23	21.86288399680319	26.854512189483557	30.723872365440126	20.55873144827313
24	21.85165553728179	26.9447526766666	29.045382791395035	22.158208994656576
25	21.9071373372699	26.901820331437705	29.95678694328307	21.234255388009327
26	21.487473662657447	27.451436912569932	30.00137053255923	21.059718892213393
27	20.939260638965397	27.331969405750296	29.690358716256824	22.038411239027482
28	21.292461740675424	27.158258531977992	29.94159549804823	21.607684229298354
29	21.556082852821316	26.971667954637024	29.480815846658874	21.99143334588279
30	21.554101359964598	27.12762795490122	30.416245599434617	20.902025085699567
31	21.99803832207185	26.957549818032906	30.2928976691039	20.751514190791344
32	21.51191207455697	26.79226028890166	29.76004121505142	21.93578642148995
33	21.229714466879347	26.760143592182352	30.202657181920863	21.80748475901744
34	20.969560967232713	26.87152000317039	30.64609877081393	21.512820258782966
35	20.62965238010317	28.05992034398716	29.881490214727773	21.428937061181895
36	22.04765820569217	26.622182152033343	29.732217752855	21.597941889419488
37	21.253244694552876	26.715642565108556	30.054953401892988	21.976159338445587
38	21.178608463616488	26.04903534322759	30.897418114807696	21.874938078348226
39	20.638156286946586	25.9890126221095	31.513992642056525	21.85883844888739
40	19.911526343947532	26.398356021426544	31.346886744473284	22.34323089015264
41	20.75126650418425	25.605015818917977	31.31732947602724	22.326388200870536
42	19.904508556746652	27.14595676382587	30.668968500868555	22.280566178558928
43	20.160286259668034	26.84898052192522	30.254671369409714	22.736061848997036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	570.0
1	457.0
2	344.0
3	563.5
4	783.0
5	783.0
6	1320.0
7	1857.0
8	2066.5
9	2276.0
10	3441.0
11	4606.0
12	4606.0
13	8791.0
14	12976.0
15	19788.0
16	26600.0
17	24555.0
18	22510.0
19	22510.0
20	24993.5
21	27477.0
22	23604.5
23	19732.0
24	21982.5
25	24233.0
26	24233.0
27	27009.5
28	29786.0
29	32694.0
30	35602.0
31	39501.5
32	43401.0
33	43401.0
34	48688.5
35	53976.0
36	57632.5
37	61289.0
38	64236.5
39	67184.0
40	67184.0
41	69298.5
42	71413.0
43	74299.5
44	77186.0
45	80005.5
46	82825.0
47	82825.0
48	96874.5
49	110924.0
50	103710.5
51	96497.0
52	88133.0
53	79769.0
54	79769.0
55	72671.5
56	65574.0
57	57733.0
58	49892.0
59	45406.0
60	40920.0
61	40920.0
62	36270.5
63	31621.0
64	27387.0
65	23153.0
66	19682.0
67	16211.0
68	16211.0
69	13766.0
70	11321.0
71	9536.5
72	7752.0
73	6340.0
74	4928.0
75	4928.0
76	3867.5
77	2807.0
78	2141.5
79	1476.0
80	1163.5
81	851.0
82	851.0
83	639.0
84	427.0
85	332.5
86	238.0
87	180.0
88	122.0
89	122.0
90	93.5
91	65.0
92	40.5
93	16.0
94	14.0
95	12.0
96	12.0
97	8.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1211208.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.076072168049265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.84440404333294	54.14727824660449
2	8.70070039425203	10.976120119608314
3	2.223555377504543	4.207594183833916
4	0.9163775891449615	2.3120599578436236
5	0.5179084320905661	1.63338148194929
6	0.34955864143517057	1.3229271656478043
7	0.2256131613576176	0.9961554433498384
8	0.176854985428626	0.8924254259340252
9	0.12785351961356117	0.7258048047075679
>10	0.8079474202370818	9.522981679988728
>50	0.06467422526941143	2.8158668955097594
>100	0.03996596101377028	4.765811814886415
>500	0.0024896782021538648	1.0840658545012043
>1k	0.0017034640330526442	1.998364152952891
>5k	1.310356948502034E-4	0.6708876594225585
>10k+	2.620713897004068E-4	1.9282751132596139
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12355	1.0200560101980833	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10975	0.9061201709367839	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8117	0.6701573965825853	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4774	0.39415195408220555	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2299	0.18981050323313584	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2274	0.18774644817405434	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2164	0.17866460591409566	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2133	0.1761051776408346	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1825	0.15067601931295038	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1798	0.14844683984914236	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1361	0.11236715741639752	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1314	0.10848673390532428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.256220236326048E-5	0.0	0.0	0.0	0.0
6	8.256220236326048E-5	0.0	0.0	0.0	0.0
7	8.256220236326048E-5	0.0	0.0	0.0	0.0
8	8.256220236326048E-5	0.0	0.0	0.0	0.0
9	8.256220236326048E-5	0.0	0.0	2.4768660708978144E-4	0.0
10	8.256220236326048E-5	0.0	0.0	4.128110118163024E-4	0.0
11	1.6512440472652096E-4	0.0	0.0	5.779354165428234E-4	0.0
12	1.6512440472652096E-4	0.0	0.0	7.430598212693443E-4	0.0
13	1.6512440472652096E-4	0.0	0.0	7.430598212693443E-4	0.0
14	1.6512440472652096E-4	0.0	0.0	0.0010733086307223862	0.0
15	1.6512440472652096E-4	0.0	0.0	0.0014861196425386886	0.0
16	1.6512440472652096E-4	0.0	0.0	0.0023117416661712934	0.0
17	1.6512440472652096E-4	0.0	0.0	0.00346761249925694	0.0
18	1.6512440472652096E-4	0.0	0.0	0.003962985713436503	0.0
19	1.6512440472652096E-4	0.0	0.0	0.0052014187488854105	0.0
20	1.6512440472652096E-4	0.0	0.0	0.009081842259958653	0.0
21	1.6512440472652096E-4	0.0	0.0	0.01560425624665623	0.0
22	1.6512440472652096E-4	0.0	0.0	0.02575940713733727	0.0
23	1.6512440472652096E-4	0.0	0.0	0.03261206993348789	0.0
24	1.6512440472652096E-4	0.0	0.0	0.043675405050164796	0.0
25	1.6512440472652096E-4	0.0	0.0	0.05019781903686237	0.0
26	1.6512440472652096E-4	0.0	0.0	0.061343716355902536	0.0
27	1.6512440472652096E-4	0.0	0.0	0.11492658568965859	0.0
28	1.6512440472652096E-4	0.0	0.0	0.2387698892345493	0.0
29	1.6512440472652096E-4	0.0	0.0	0.4026558609256214	0.0
30	1.6512440472652096E-4	0.0	0.0	0.6638001070006143	0.0
31	1.6512440472652096E-4	0.0	0.0	1.0307065343029438	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACCGT	40	0.0019312473	23.125	6
GGTATCA	3490	0.0	22.528654	1
TTGACGC	45	0.0038259255	20.555555	11
GTATTGG	240	0.0	19.270834	1
TCGTCCG	50	0.0070350952	18.5	10
TCTATCG	60	9.237205E-4	18.5	31
TTATGCG	135	1.1514203E-9	17.814816	4
TTAACGG	145	2.9795046E-9	16.586208	35
GTATTAG	270	0.0	16.444445	1
TATTGCG	90	4.4462748E-5	16.444445	11
TATGCGG	150	4.671165E-9	16.033333	5
TATACTG	150	4.671165E-9	16.033333	5
CGCCTAT	70	0.0025926006	15.857143	36
TAGGCGA	70	0.0025926006	15.857143	33
TATCGCC	70	0.0025926006	15.857143	33
CGATAGA	70	0.0025926006	15.857143	37
ATAGGCG	95	7.060174E-5	15.578948	32
GTATCAA	5230	0.0	15.068834	2
TCGCCTA	75	0.004104575	14.8	35
GCGCCAA	75	0.004104575	14.8	13
>>END_MODULE