FastQCFastQC Report
Wed 25 May 2016
SRR2088644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421666
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65181.5457731948983318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56231.3335198948931144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34960.8290922199086481No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.5596846793433666No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG12800.3035577921862327No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG12070.28624551185061164No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA11930.2829253484985747No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA9800.2324114346425844No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9680.22956558034083846No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG7600.1802374391105757No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.1702769490544649No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.15960499542291764No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.12332035307565704No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC4990.11834010804760166No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA4930.11691718089672869No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA4710.11169978134352782No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC4600.10909108156692737No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG4330.10268790938799902No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA4320.10245075486285354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGT200.001840614637.08
CGATCTG250.00549244529.629
GTTATGG405.931348E-527.7500021
GGTATCA15600.024.9038471
TACACTG751.3684257E-824.6666645
ATCACGT603.719089E-521.58333224
AGTAGTG656.8888905E-519.9230775
GGGTAGA500.007027675418.51
TAGTACT609.2236017E-418.54
TCACGTA500.007027675418.525
TCTTGGG752.0633583E-417.2666662
GACCCTC2050.017.1463437
GTATCAA23000.016.810872
TTGGACC2100.016.7380964
ACTGTCC803.3762155E-416.18758
TAAGAAC803.3762155E-416.18753
GACCATG700.002588845315.8571427
CGGTCTG700.002588845315.85714210
ATACACT700.002588845315.8571424
GGACCCT2250.015.6222226
Produced by FastQC (version 0.11.5)