##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2088644_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 421666 Sequences flagged as poor quality 0 Sequence length 43 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93057538430891 31.0 31.0 33.0 28.0 34.0 2 31.146888769784617 31.0 31.0 34.0 28.0 34.0 3 31.154100638894292 31.0 31.0 34.0 28.0 34.0 4 34.95492641095085 35.0 35.0 37.0 32.0 37.0 5 34.76668737816186 35.0 35.0 37.0 32.0 37.0 6 34.92821806832896 35.0 35.0 37.0 32.0 37.0 7 34.77893640938563 35.0 35.0 37.0 32.0 37.0 8 34.843689080931355 35.0 35.0 37.0 32.0 37.0 9 36.32025821384698 38.0 35.0 39.0 32.0 39.0 10 36.034714679390795 38.0 35.0 39.0 31.0 39.0 11 36.21814184686458 38.0 35.0 39.0 32.0 39.0 12 35.99461184918869 37.0 35.0 39.0 30.0 39.0 13 36.100906878904155 38.0 35.0 39.0 32.0 39.0 14 36.84992861648793 38.0 36.0 40.0 31.0 41.0 15 36.903267040738406 38.0 36.0 40.0 31.0 41.0 16 36.7389616426271 38.0 36.0 40.0 31.0 41.0 17 36.80542894138963 38.0 36.0 40.0 31.0 41.0 18 36.7810091399354 38.0 36.0 40.0 31.0 41.0 19 36.82888352392652 39.0 36.0 40.0 31.0 41.0 20 36.714397176912534 39.0 35.0 40.0 30.0 41.0 21 36.637563853855895 38.0 35.0 40.0 30.0 41.0 22 36.49170907780091 38.0 35.0 40.0 30.0 41.0 23 36.28282100050751 38.0 35.0 40.0 30.0 41.0 24 36.12017331252698 38.0 34.0 40.0 29.0 41.0 25 36.28779887399031 38.0 35.0 40.0 30.0 41.0 26 36.10314561762153 38.0 35.0 40.0 29.0 41.0 27 35.95216118918765 38.0 34.0 40.0 29.0 41.0 28 35.74827707237482 38.0 34.0 40.0 27.0 41.0 29 35.63380258308709 38.0 34.0 40.0 27.0 41.0 30 35.38916820421851 38.0 34.0 40.0 27.0 41.0 31 35.227177434272626 38.0 34.0 40.0 27.0 41.0 32 35.0179336251915 37.0 33.0 40.0 26.0 41.0 33 34.84849620315605 37.0 33.0 40.0 25.0 41.0 34 34.62681838232155 37.0 33.0 40.0 25.0 41.0 35 34.45513985002348 37.0 33.0 40.0 24.0 41.0 36 34.263689744964026 37.0 33.0 40.0 24.0 41.0 37 34.167348090668916 37.0 33.0 40.0 23.0 41.0 38 33.91790658957564 37.0 33.0 40.0 22.0 41.0 39 33.78640677692771 37.0 33.0 40.0 21.0 41.0 40 33.57992344651928 37.0 32.0 40.0 20.0 41.0 41 33.433570645961495 37.0 32.0 40.0 19.0 41.0 42 33.121868967381765 37.0 31.0 40.0 18.0 41.0 43 32.72376478065578 36.0 31.0 40.0 15.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 1.0 15 5.0 16 12.0 17 37.0 18 74.0 19 170.0 20 366.0 21 670.0 22 1061.0 23 1815.0 24 2807.0 25 4167.0 26 5713.0 27 7622.0 28 9871.0 29 12661.0 30 15808.0 31 18685.0 32 22330.0 33 26964.0 34 31964.0 35 38886.0 36 49700.0 37 63428.0 38 71540.0 39 35307.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.13634487959664 25.09237168754417 16.074570868886752 20.696712563972433 2 21.34461872666992 25.805732499181815 32.15933938235476 20.690309391793505 3 22.276161701441424 25.364150773360905 32.18542638012076 20.174261145076912 4 16.717971095606476 18.742796431298707 33.83910488396029 30.700127589134528 5 12.370217186114129 40.51713915753227 34.0487494841889 13.0638941721647 6 32.07277798067665 38.32535703613761 13.194566315519868 16.407298667665877 7 24.829604473682963 34.16116072910787 21.60809740410657 19.401137393102598 8 27.059568473626044 35.85064956624437 18.731650168616866 18.358131791512715 9 24.29671825568104 14.402157157560724 22.059402465458444 39.2417221212998 10 16.294887422746914 29.313959389659118 33.29768110305313 21.093472084540846 11 33.299341184729144 22.74762489743067 23.100985139897453 20.852048777942734 12 19.873074898142132 28.594669714892827 31.499575493399988 20.03267989356505 13 32.02273837587095 21.218452519292523 26.59047682288826 20.168332281948274 14 19.626671346515963 23.617982004714634 28.863840100932965 27.891506547836435 15 22.440272632842106 30.670720428016484 25.884704956055266 21.00430198308614 16 20.881930248111065 28.78273325333321 27.71411496302761 22.621221535528118 17 21.245488135159107 27.75988578638069 28.058226179013722 22.93639989944648 18 22.04161587607253 25.978380993487736 30.511589741643864 21.46841338879587 19 22.02027196880944 25.42604810442388 32.12803498503555 20.425644941731132 20 22.810945155644514 25.175138616819947 31.745504735975867 20.26841149155967 21 21.759876300199686 25.72557426968264 32.35854918347697 20.15600024664071 22 20.537581877599806 26.16929038622986 31.78487238715002 21.508255349020313 23 21.466753307119856 26.32770960902705 32.54850995811851 19.657027125734587 24 21.231733172700668 26.363757096849167 30.652459529580284 21.75205020086988 25 21.296476358065387 26.66233464400734 31.402579292615485 20.638609705311787 26 21.14422315292198 27.294351453520083 31.248191696745764 20.31323369681217 27 20.14841130183605 27.186446144578884 31.196729164789193 21.46841338879587 28 20.740348996599202 27.188580535305196 31.22589917138209 20.845171296713513 29 20.847068532914676 26.411188001878266 30.79688663539389 21.94485682981317 30 20.846594223864383 26.89972632367798 32.10740254134789 20.14627691110974 31 21.533156574160593 26.797275568815127 31.793409950055256 19.876157906969024 32 21.140902989569945 26.584785114284763 30.99491066389038 21.279401232254912 33 20.84422267861293 26.699567904455186 31.430800681107797 21.02540873582409 34 20.25465652910123 27.09348157072185 31.95894380860681 20.692918091570107 35 19.812363339704888 28.643997856123093 31.049693359198987 20.493945444973036 36 21.79426370634578 26.41284808355428 30.968586511599227 20.824301698500708 37 20.90896586397765 27.29055698111776 30.908586416737414 20.891890738167174 38 20.930546925765892 25.799566481528036 32.07799538022985 21.191891212476225 39 20.276949054464907 25.81806453448938 32.72756162460336 21.17742478644235 40 19.409200646957544 26.438460772269995 32.18376629844474 21.96857228232772 41 20.516712279387004 25.66391409314481 32.23807468470306 21.581298942765127 42 19.771098452329568 27.241703148937784 31.341393425127944 21.6458049736047 43 19.9615335360214 27.20992444256829 30.606688706227203 22.221853315183107 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170.0 1 146.0 2 122.0 3 172.0 4 222.0 5 222.0 6 414.0 7 606.0 8 713.5 9 821.0 10 1342.0 11 1863.0 12 1863.0 13 3803.0 14 5743.0 15 9382.0 16 13021.0 17 11352.0 18 9683.0 19 9683.0 20 11040.0 21 12397.0 22 9913.0 23 7429.0 24 8165.5 25 8902.0 26 8902.0 27 9679.0 28 10456.0 29 11286.5 30 12117.0 31 13172.5 32 14228.0 33 14228.0 34 15763.0 35 17298.0 36 18507.5 37 19717.0 38 20808.5 39 21900.0 40 21900.0 41 22530.5 42 23161.0 43 24030.5 44 24900.0 45 26295.0 46 27690.0 47 27690.0 48 35111.5 49 42533.0 50 39030.5 51 35528.0 52 32128.0 53 28728.0 54 28728.0 55 25459.0 56 22190.0 57 19173.0 58 16156.0 59 14422.5 60 12689.0 61 12689.0 62 11290.0 63 9891.0 64 8518.0 65 7145.0 66 6063.5 67 4982.0 68 4982.0 69 4257.0 70 3532.0 71 2941.0 72 2350.0 73 1921.0 74 1492.0 75 1492.0 76 1202.0 77 912.0 78 702.0 79 492.0 80 381.0 81 270.0 82 270.0 83 215.5 84 161.0 85 123.5 86 86.0 87 65.5 88 45.0 89 45.0 90 34.0 91 23.0 92 18.0 93 13.0 94 6.5 95 0.0 96 0.0 97 1.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 421666.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.88041076184 #Duplication Level Percentage of deduplicated Percentage of total 1 89.35900334365607 60.65725852236007 2 6.15648256707763 8.358091310026733 3 1.7253235633752324 3.513470165369767 4 0.7657161263520306 2.079085007349632 5 0.472501017018198 1.6036781560291216 6 0.30795203893430095 1.2542346538683893 7 0.20741406551601776 0.9855546375507334 8 0.17014637409967107 0.9239684610818693 9 0.11671401889545648 0.7130335989859866 >10 0.6479597078661011 7.807862934314662 >50 0.036795314893782495 1.7012776330977246 >100 0.029436251902130808 4.147339978038358 >500 0.0021025894215807722 1.0987418043337642 >1k 0.00175215785131731 2.2683701766890616 >5k 7.008631405269241E-4 2.888032960904142 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 6518 1.5457731948983318 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5623 1.3335198948931144 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3496 0.8290922199086481 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2360 0.5596846793433666 No Hit TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG 1280 0.3035577921862327 No Hit GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG 1207 0.28624551185061164 No Hit GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA 1193 0.2829253484985747 No Hit ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA 980 0.2324114346425844 No Hit ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA 968 0.22956558034083846 No Hit GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG 760 0.1802374391105757 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 718 0.1702769490544649 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 673 0.15960499542291764 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 520 0.12332035307565704 No Hit CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC 499 0.11834010804760166 No Hit TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA 493 0.11691718089672869 No Hit CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA 471 0.11169978134352782 No Hit CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC 460 0.10909108156692737 No Hit ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG 433 0.10268790938799902 No Hit GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA 432 0.10245075486285354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.371545251454943E-4 0.0 0.0 0.0 0.0 6 2.371545251454943E-4 0.0 0.0 0.0 0.0 7 2.371545251454943E-4 0.0 0.0 0.0 0.0 8 2.371545251454943E-4 0.0 0.0 0.0 0.0 9 2.371545251454943E-4 0.0 0.0 4.743090502909886E-4 0.0 10 2.371545251454943E-4 0.0 0.0 4.743090502909886E-4 0.0 11 2.371545251454943E-4 0.0 0.0 7.114635754364829E-4 0.0 12 2.371545251454943E-4 0.0 0.0 7.114635754364829E-4 0.0 13 2.371545251454943E-4 0.0 0.0 9.486181005819772E-4 0.0 14 2.371545251454943E-4 0.0 0.0 9.486181005819772E-4 0.0 15 2.371545251454943E-4 0.0 0.0 0.0011857726257274714 0.0 16 2.371545251454943E-4 0.0 0.0 0.0014229271508729659 0.0 17 2.371545251454943E-4 0.0 0.0 0.002371545251454943 0.0 18 2.371545251454943E-4 0.0 0.0 0.0028458543017459317 0.0 19 2.371545251454943E-4 0.0 0.0 0.004505935977764392 0.0 20 2.371545251454943E-4 0.0 0.0 0.00877471743038329 2.371545251454943E-4 21 2.371545251454943E-4 0.0 0.0 0.01612650770989361 2.371545251454943E-4 22 2.371545251454943E-4 0.0 0.0 0.029407161118041293 2.371545251454943E-4 23 2.371545251454943E-4 0.0 0.0 0.03628464234726063 2.371545251454943E-4 24 2.371545251454943E-4 0.0 0.0 0.047193750503953365 2.371545251454943E-4 25 2.371545251454943E-4 0.0 0.0 0.05075106838113578 2.371545251454943E-4 26 2.371545251454943E-4 0.0 0.0 0.06071155843724654 2.371545251454943E-4 27 2.371545251454943E-4 0.0 0.0 0.10600807274003596 2.371545251454943E-4 28 2.371545251454943E-4 0.0 0.0 0.20585012782628906 2.371545251454943E-4 29 2.371545251454943E-4 0.0 0.0 0.33035625352767356 2.371545251454943E-4 30 2.371545251454943E-4 0.0 0.0 0.5442696352089095 2.371545251454943E-4 31 2.371545251454943E-4 0.0 0.0 0.8191317298525373 2.371545251454943E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTGT 20 0.0018406146 37.0 8 CGATCTG 25 0.005492445 29.6 29 GTTATGG 40 5.931348E-5 27.750002 1 GGTATCA 1560 0.0 24.903847 1 TACACTG 75 1.3684257E-8 24.666664 5 ATCACGT 60 3.719089E-5 21.583332 24 AGTAGTG 65 6.8888905E-5 19.923077 5 GGGTAGA 50 0.0070276754 18.5 1 TAGTACT 60 9.2236017E-4 18.5 4 TCACGTA 50 0.0070276754 18.5 25 TCTTGGG 75 2.0633583E-4 17.266666 2 GACCCTC 205 0.0 17.146343 7 GTATCAA 2300 0.0 16.81087 2 TTGGACC 210 0.0 16.738096 4 ACTGTCC 80 3.3762155E-4 16.1875 8 TAAGAAC 80 3.3762155E-4 16.1875 3 GACCATG 70 0.0025888453 15.857142 7 CGGTCTG 70 0.0025888453 15.857142 10 ATACACT 70 0.0025888453 15.857142 4 GGACCCT 225 0.0 15.622222 6 >>END_MODULE