FastQCFastQC Report
Wed 25 May 2016
SRR2088643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961970
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91870.9550193872989802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76550.7957628616277015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66000.6860920818736551No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33020.3432539476283044No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22640.23535037475181136No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20930.21757435263053942No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG20920.217470399284801No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG18800.19543228998825327No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA16780.1744337141490899No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG15310.15915257232554028No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12030.12505587492333442No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA10660.11081426655716914No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA9850.10239404555235611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATCG652.6820908E-622.7692319
GGTATCA24800.022.0806471
TATAGAC806.957689E-720.81253
AGCCTAC500.00703406918.56
TAATACT2100.017.6190474
GTATTGG5050.017.584161
TACACTA852.7223678E-517.4117645
TAAGCCG752.0668253E-417.26666610
ATATAAC1201.04064384E-716.9583323
ATTAGAC1107.8043377E-716.8181823
ACCGTTA1655.4569682E-1116.8181828
CTTAATA803.3818482E-416.18752
GCGATTT700.002592080715.85714330
TCTATGC700.002592080715.8571433
CGCCGTT1902.7284841E-1115.57894825
CGTTTAT957.0577626E-515.5789481
GACCGTT1701.4842954E-915.2352947
ATACTGC1101.4513002E-515.1363646
ATAACTG2201.8189894E-1215.1363645
AATACTG2201.8189894E-1215.1363645
Produced by FastQC (version 0.11.5)